Pepsinogens and pepsins from house musk shrew, Suncus murinus: Purification, characterization, determination of the amino-acid sequences of the activation segments, and analysis of proteolytic specificities

Journal of Biochemistry

Volumn 121, Issue 6, 1997, Pages 1010-1017

  Narita, Yuichi ^a   Oda, Sen Ichi ^a   Moriyama, Akihiko ^b   Takenaka, Osamu ^c   Kageyama, Takashi ^c  

^a NAGOYA UNIVERSITY   (Japan)

^b NAGOYA CITY UNIVERSITY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Activation segment; House musk shrew; Pepsin; Pepsinogen; Proteolytic specificity

Indexed keywords

AROMATIC AMINO ACID; PEPSIN A; PEPSINOGEN; PEPSTATIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; GEL FILTRATION; INSECTIVORE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; STOMACH MUCOSA;

ANIMALIA; SUNCUS; SUNCUS MURINUS;

EID: 0030798898     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021687     Document Type: Article

References (47)

1. Foltmann, B. (1981) Gastric proteinases - structure, function, evolution and mechanism of action. Essays Biochem. 17, 52-84
2. Takahashi, K., Tanji, M., Yakabe, E., Hirasawa, A., Athauda, S. B.P., and Kageyama, T. (1995) Non-mammalian vertebrate pepsinogens and pepsins: isolation and characterization in Aspartic Proteinases: Structure, Function, Biology, and Biomedical Implications (Takahashi, K., ed.) pp. 53-65, Plenum Press, New York
3. Kageyama, T., Tanabe, K., and Koiwai, O. (1990) Structure and development of rabbit pepsinogens. Stage-specific zymogens, nucleotide sequences of cDNAs, molecular evolution, and gene expression during development. J. Biol. Chem. 265, 17031-17038
4. 2-terminal 60-residue sequence of Japanese monkey progastricsin, and molecular evolution of pepsinogens. J. Biochem. 97, 1235-1246
5. Tang, J. (1970) Gastricsin and pepsin. Methods Enzymol. 19, 406-421
6. Kageyama, T. (1994) Asian macaque pepsinogens and pepsins. J. Med. Primatol. 23, 375-381
7. Chow, R.B. and Kassell, B. (1968) Bovine pepsinogen and pepsin. Isolation, purification, and some properties of the pepsinogen. J. Biol. Chem. 243, 1718-1724
8. Martin, P., Trieu-Cuot, P., Collin, J-C., and Ribadeau Dumas, B. (1982) Purification and characterization of bovine gastricsin. Eur. J. Biochem. 122, 31-39
9. Ryle, A.P. (1970) The porcine pepsins and pepsinogens. Methods Enzymol. 19, 316-336
10. 2-terminal amino-acid sequences. Eur. J. Biochem. 141, 261-269
11. 2-terminal 60 residues of the major pepsinogen. J. Biochem. 94, 1557-1567
12. Esumi, H., Sato, S., Sugimura, T., and Furihata, C. (1978) Purification of mouse pepsinogens by pepstatin-affinity chromatography. FEBS Lett. 86, 33-36
13. Furihata, C., Saito, D., Fujiki, H., Kanai, Y., Matsushima, T., and Sugimura, T. (1980) Purification and characterization of pepsinogens and a unique pepsin from rat stomach. Eur. J. Biochem. 105, 43-50
14. Kageyama, T., Ichinose, M., Tsukada, S., Miki, K., Kurokawa, K., Koiwai, O., Tanji, M., Yakabe, E., Athauda, S.B.P., and Takahashi, K. (1992) Gastric procathepsin E and progastricsin from guinea pig. Purification, molecular cloning of cDNAs, and characterization of enzymatic properties, with special reference to procathepsin E. J. Biol. Chem. 267, 16450-16459
15. Kageyama, T. and Takahashi, K. (1982) Monkey pepsinogens and pepsins. VI. One-step activation of Japanese monkey pepsinogen to pepsin. J. Biochem. 92, 1179-1188
16. Takahashi, K. and Kageyama, T. (1985) Multiplicity and intermediates of the activation mechanism of zymogens of gastric aspartic proteinases in Aspartic Proteinases and Their Inhibitors (Kostka, V., ed.) pp. 265-282, Walter de Gruyter & Co., Berlin
17. Kageyama, T. and Takahashi, K. (1983) Occurrence of two different, pathways in the activation of porcine pepsinogen to pepsin. J. Biochem. 93, 743-754
18. Kageyama, T. and Takahashi, K. (1980) Isolation of an activation intermediate and determination of the amino acid sequence of the activation segment of human pepsinogen A. J. Biochem. 88, 571-582
19. Kageyama, T., Ichinose, M., Miki, K., Athauda, S.B., Tanji, M., and Takahashi, K. (1989) Difference of activation processes and structure of activation peptides in human pepsinogens A and progastricsin. J. Biochem. 105, 15-22
20. Foltmann, B. and Jensen, A.L. (1982) Human progastricsin. Analysis of intermediates during activation into gastricsin and determination of the amino acid sequence of the propart. Eur. J. Biochem. 128, 63-70
21. Shigehara, N. (1985) A historical review of the classification of mammalian order Insectivora (in Japanese with English summary) in Suncus murinus, Biology of the Laboratory Shrew (Oda, S., Kitoh, J., Ota, K., and Isomura, G., eds.) pp. 11-19, Japan Sci. Soc. Press, Tokyo
22. Novacek, M.J. (1992) Mammalian phylogeny: shaking the tree. Nature 356, 121-125
23. Oda, S., Koyasu, K., and Shrestha, K.C. (1992) Breeding of the house musk shrew, Suncus murinus originating from a wild population in Katmandu, Nepal (in Japanese). Ann. Res. Inst. Environ. Med. 43, 239-240
24. Anson, M.L. and Mirsky, A.E. (1932) The estimation of pepsin with hemoglobin. J. Gen. Physiol. 16, 59-63
25. Kageyama, T. and Takahashi, K. (1976) Pepsinogen C and pepsin C from gastric mucosa of Japanese monkey. Purification and characterization. J. Biochem. 80, 983-992
26. Ornstein, L. (1964) Disc electrophoresis. I. Background and theory. Ann. N.Y. Acad. Sci. 121, 321-349
27. Davis, B.J. (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404-427
28. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
29. 2-terminal amino acid sequences and some other properties of six isozymic forms of human pepsinogens and pepsins. J. Biochem. 106, 920-927
30. Sogawa, K., Fujii-Kuriyama, Y., Mizukami, Y., Ichihara, Y., and Takahashi, K. (1983) Primary structure of human pepsinogen gene. J. Biol. Chem. 258, 5306-5311
31. Zelle, B., Evers, M.P.J., Groot, P.C., Bebelman, J.P., Mager, W. H., Planta, R.J., Pronk, J.C. Meuwissen, S.G.M., Hofker, M.H., Eriksson, A.W., and Frants, R.R. (1988) Genomic structure and evolution of the human pepsinogen A multigene family. Hum. Genet. 78, 79-82
32. Hayano, T., Sogawa, K., Ichihara, Y., Fujii-Kuriyama, Y., and Takahashi, K. (1988) Primary structure of human pepsinogen C gene. J. Biol. Chem. 263, 1382-1385
33. Pals, G., Azuma, T., Mohandas, T.K., Bell, G.I., Bacon, J., Samloff, I.M., Walz, D.A., Barr, P.J., and Taggart, R.T. (1989) Human pepsinogen C (progastricsin) polymorphism: evidence for a single locus located at 6p21.1-pter. Genomics 4, 137-145
34. Kageyama, T. and Takahashi, K. (1978) Monkey pepsinogens and pepsins. III. Carbohydrate moiety of Japanese monkey pepsinogens and the amino acid sequence around the site of its attachment to protein. J. Biochem. 84, 771-778
35. Baudys, M. and Kostka, V. (1983) Covalent structure of chicken pepsinogen. Eur. J. Biochem. 136, 89-99
36. Hayashi, K., Agata, K., Mochii, M., Yasugi, S., Eguchi, G., and Mizuno, T. (1988) Molecular cloning and the nucleotide sequence of cDNA for embryonic chicken pepsinogen: phylogenetic relationship with prochymosin. J. Biochem. 103, 290-296
37. Hirasawa, A., Athauda, S.B.P., and Takahashi, K. (1996) Purification and characterization of turtle pepsinogen and pepsin. J. Biochem. 120, 407-414
38. Yakabe, E., Tanji, M., Ichinose, M., Goto, S., Miki, K., Kurokawa, K., Ito, H., Kageyama, T., and Takahashi, K. (1991) Purification, characterization, and amino acid sequences of pepsinogens and pepsins from the esophageal mucosa of bullfrog (Rana catesbeiana). J. Biol. Chem. 266, 22436-22443
39. James, M.N.G. and Sielecki, A.R. (1986) Molecular structure of an aspartic proteinase zymogen, porcine pepsinogen, at 1.8 A resolution. Nature 319, 33-38
40. Sielecki, A.R., Fedorov, A.A., Boodhoo, A., Andreeva, N.S., and James, M.N.G. (1990) Molecular and crystal structures of monoclinic porcine pepsin refined at 1.8 A resolution. J. Mol. Biol. 214, 143-170
41. Hartsuck, J.A., Koelsch, G., and Remington, S.J. (1992) The high-resolution crystal structure of porcine pepsinogen. Proteins: Struct. Funct. Genet. 13, 1-25
42. Harboe, M., Andersen, P.M., Foltmann, B., Kay, J., and Kassell, B. (1974) The activation of bovine pepsinogen. Sequence of the peptides released, identification of a pepsin inhibitor. J. Biol. Chem. 249, 4487-4494
43. Fujinaga, M., Chernaia, M.M., Tarasova, N.I., Mosimann, S.C., and James, M.N.G. (1995) Crystal structure of human pepsin and its complex with pepstatin. Protein Sci. 4, 960-972
44. Kageyama, T. and Takahashi, K. (1986) The complete amino acid sequence of monkey pepsinogen A. J. Biol. Chem. 261, 4395-4405
45. Kageyama, T. and Takahashi, K. (1986) The complete amino acid sequence of monkey progastricsin. J. Biol. Chem. 261, 4406-4419
46. Tsukagoshi, N., Ando, Y., Tomita, Y., Uchida, R., Takemura, T., Sasaki, T., Yamagata, H., Udaka, S., Ichihara, Y., and Takahashi, K. (1988) Nucleotide sequence and expression in Escherichia coli of cDNA of swine pepsinogen: involvement of the amino-terminal portion of the activation peptide segment in restoration of the functional protein. Gene 65, 285-292
47. Ichihara, Y., Sogawa, K., Morohashi, K., Fujii-Kuriyama, Y., and Takahashi K. (1986) Nucleotide sequence of a nearly full-length cDNA coding for pepsinogen of rat gastric mucosa. Eur. J. Biochem. 161, 7-12