Induction of cell migration by matrix metalloprotease-2 cleavage of laminin-5

Science

Volumn 277, Issue 5323, 1997, Pages 225-228

  Giannelli, Gianluigi ^a,b   Falk Marzillier, Jutta ^a   Schiraldi, Oronzo ^b   Stetler Stevenson, William G ^c   Quaranta, Vito ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF BARI   (Italy)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GELATINASE A; KALININ;

ARTICLE; BREAST EPITHELIUM; CANCER INVASION; CELL MIGRATION; CONTROLLED STUDY; ENZYME INDUCTION; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; PRIORITY JOURNAL; TISSUE REGENERATION;

ANIMALS; BREAST; CELL ADHESION; CELL ADHESION MOLECULES; CELL DIVISION; CELL LINE; CELL MOVEMENT; CELL SIZE; COLLAGENASES; EPITHELIAL CELLS; EPITHELIUM; EXTRACELLULAR MATRIX; FEMALE; GELATINASES; HUMANS; MATRIX METALLOPROTEINASE 2; MATRIX METALLOPROTEINASE 9; METALLOENDOPEPTIDASES; MICE; PHENYLALANINE; PLASMIN; PROTEASE INHIBITORS; RATS; RECOMBINANT FUSION PROTEINS; SKIN NEOPLASMS; THIOPHENES;

EID: 0030798679     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5323.225     Document Type: Article

References (37)

1. H. Birkedal-Hansen, Curr. Opin. Cell Biol. 7, 728 (1995); J. Gailit and R. A. Clark, ibid. 6, 717 (1994); W. T. Chen, ibid. 4, 802 (1992); E. C. Kohn and L. A. Liotta, Cancer Res. 55, 1856 (1995).
2. H. Birkedal-Hansen, Curr. Opin. Cell Biol. 7, 728 (1995); J. Gailit and R. A. Clark, ibid. 6, 717 (1994); W. T. Chen, ibid. 4, 802 (1992); E. C. Kohn and L. A. Liotta, Cancer Res. 55, 1856 (1995).
3. H. Birkedal-Hansen, Curr. Opin. Cell Biol. 7, 728 (1995); J. Gailit and R. A. Clark, ibid. 6, 717 (1994); W. T. Chen, ibid. 4, 802 (1992); E. C. Kohn and L. A. Liotta, Cancer Res. 55, 1856 (1995).
4. H. Birkedal-Hansen, Curr. Opin. Cell Biol. 7, 728 (1995); J. Gailit and R. A. Clark, ibid. 6, 717 (1994); W. T. Chen, ibid. 4, 802 (1992); E. C. Kohn and L. A. Liotta, Cancer Res. 55, 1856 (1995).
5. P. C. Brooks et al., Cell 85, 683 (1996); H. Sato et al., Nature 370, 61 (1994); C. B. Basbaum and Z. Werb, Curr. Opin. Cell Biol. 8, 731 (1996).
6. P. C. Brooks et al., Cell 85, 683 (1996); H. Sato et al., Nature 370, 61 (1994); C. B. Basbaum and Z. Werb, Curr. Opin. Cell Biol. 8, 731 (1996).
7. P. C. Brooks et al., Cell 85, 683 (1996); H. Sato et al., Nature 370, 61 (1994); C. B. Basbaum and Z. Werb, Curr. Opin. Cell Biol. 8, 731 (1996).
8. R. E. Burgeson et al., Matrix Biol. 14, 209 (1994); G. W. Laurie, C. P. Leblond, G. R. Martin, J. Cell Biol. 95, 340 (1982).
9. R. E. Burgeson et al., Matrix Biol. 14, 209 (1994); G. W. Laurie, C. P. Leblond, G. R. Martin, J. Cell Biol. 95, 340 (1982).
10. S. E. Baker et al., J. Cell Sci. 109, 2509 (1996).
11. K. Miyazaki, Y. Kikkawa, A. Nakamura, H. Yasumitsu, M. Umeda, Proc. Natl. Acad. Sci. U.S.A. 90, 11767 (1993).
12. P. Basset et al., Nature 348, 699 (1990); C. Monteagudo, M. J. Merino, J. San-Juan, L. A. Liotta, W. G. Stetler-Stevenson, Am. J. Pathol. 136, 585 (1990).
13. P. Basset et al., Nature 348, 699 (1990); C. Monteagudo, M. J. Merino, J. San-Juan, L. A. Liotta, W. G. Stetler-Stevenson, Am. J. Pathol. 136, 585 (1990).
14. A. J. Pelletier, T. Kunicki, V. Quaranta, J. Biol. Chem. 271, 1364 (1996).
15. H. D. Soule et al., Cancer Res. 50, 6075 (1990).
16. G. Giannelli and V. Quaranta, unpublished data.
17. X. Wang, X. Fu, P. D. Brown, M. J. Crimmin, R. M. Hoffman, Cancer Res. 54, 4726 (1994).
18. M. Hormia et al., J. Invest. Dermatol. 105, 557 (1995).
19. G. Plopper et al., J. Cell Sci. 109, 1965 (1996).
20. A. L. Calof, M. R. Campanero, J. J. O'Rear, P. D. Yurchenco, A. D. Lander, Neuron 13, 117 (1994); S. Stefansson and D. A. Lawrence, Nature 383, 441 (1996).
21. A. L. Calof, M. R. Campanero, J. J. O'Rear, P. D. Yurchenco, A. D. Lander, Neuron 13, 117 (1994); S. Stefansson and D. A. Lawrence, Nature 383, 441 (1996).
22. M. Aumailley, M. Gerl, A. Sonnenberg, R. Deutzmann, R. Timpl, FEBS Lett. 262, 82 (1990); P. I. Karecla, R. Timpl, F. M. Watt, Cell Adhes. Commun. 2, 309 (1994).
23. M. Aumailley, M. Gerl, A. Sonnenberg, R. Deutzmann, R. Timpl, FEBS Lett. 262, 82 (1990); P. I. Karecla, R. Timpl, F. M. Watt, Cell Adhes. Commun. 2, 309 (1994).
24. D. A. Lauffenburger and A. F. Horwitz, Cell 84, 359 (1996).
25. Y. Itoh, S. Binner, H. Nagase, Biochem. J. 308, 645 (1995).
26. S. M. Wilhelm et al., J. Biol. Chem. 264, 17213 (1989).
27. G. Giannelli et al., Lab. Invest. 74, 1091 (1996).
28. M. Langhofer, S. B. Hopkinson, J. C. R. Jones, J. Cell Sci. 105, 753 (1993).
29. C. A. Burdsal, M. C. Alliegro, D. R. McClay, Dev. Biol. 144, 327 (1991).
30. R. Fridman et al., Biochem. J. 289, 411 (1993).
31. For migration assays (7), transwell filters were coated on the underside with Ln-5 (1 μg/ml), Coll IV (10 μg/ml, Gibco-BRL), Ln-1 (20 μg/ml; Collaborative, Becton-Dickinson, Bedford, MA), or Fn (20 μg/ml, Gibco-BRL). Filters were washed and cells were plated in the upper transwell chamber. After 16 hours at 37°C, filters were fixed and stained, and cells that migrated to the underside were quantified by counting four microscopic fields from duplicate filters. Results are the mean number of cells counted in each field ± SD. In some experiments (Fig. 3C), after blocking, filters were incubated with indicated mAbs(100 μg) (12). In Fig. 3, D to F, filters precoated with Ln-1, Coll IV, or Fn were recoated with MMP2-cleaved Ln-5 followed by addition of BB94 and mAbs.
32. 2-terminal sequence from the 80-kD polypeptide was obtained by automated Edman degradation. Rat γ2 cDNA was cloned by homology screening and sequenced from an 804G cell λgt11 cDNA library (Clontech, Palo Alto, CA).
33. M. P. Marinkovich, G. P. Lunstrum, R. E. Burgeson, J. Biol. Chem. 267, 17900 (1992).
34. C. Pyke et al., Cancer Res. 55, 4132 (1995).
35. MCF-10 cells were plated for 2 hours at 37°C on glass cover slips coated with Ln-5 (19), washed, fixed, and photographed with an Axiovert 135 microscope (Zeiss).
36. 2 for 30 min, filled with phosphate-buffered saline (PBS), inverted, and gently shaken in a tank of PBS for 15 min. Excess PBS was removed, and adherent cells were fixed, stained with crystal violet, solubilized, and quantified by densitometry (11). To measure strength of adhesion, we performed a detachment assay (20). Radio-labeled cells were incubated on matrix-coated polystyrene plates and were detached by inverted centrifugation for 8 min at 80, 1200, 1450, or 1700g; the remaining cells were quantified on a Molecular Dynamics Phosphorimager.
37. We thank British Bio-Technology Ltd. for BB94, Desmos Inc. for purified Ln-5, and K. Tryggvason for antisera; Z. Werb, H. Gardner, A. Pozzi, G. Plopper, A. Pelletier, F. Frasier, and S. Carter for technical support, assistance, and discussions; L. Bibbs for help with microsequencing; and Z. Ruggeri for help with microscopy. Supported by NIH grants DE10063 and CA47858 (V.Q.), an Istituto Superiore di Sanità fellowship (G.G.), and a Deutsche Forschungsgemeinschaft fellowship (J.F.-M.).