Involvement of the amino-terminal phosphorylation module of UhpA in activation of uhpT transcription in Escherichia coli

Molecular Microbiology

Volumn 24, Issue 5, 1997, Pages 1039-1048

  Webber, Carol A ^a   Kadner, Robert J ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ARGININE; ASPARTIC ACID; BACTERIAL PROTEIN; GLYCINE; HISTIDINE; LEUCINE; LYSINE; PHENYLALANINE; SUGAR PHOSPHATE; VALINE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; DNA FLANKING REGION; ESCHERICHIA COLI; GENE OVEREXPRESSION; NONHUMAN; OLIGOMERIZATION; PHOSPHATE TRANSPORT; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SITE DIRECTED MUTAGENESIS; TRANSACTIVATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030797034     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.4021765.x     Document Type: Article

References (29)

1. Baikalov, I., Schröder, I., Kaczor-Grzeskowiak, M., Grzeskowiak, K., Gunsalus, R.P., and Dickerson, R.E. (1996) Structure of the Escherichia coli response regulator NarL. Biochem 35: 11053-11061.
2. Bowrin, V., Brissette, R., and Inouye, M. (1992) Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system. J Bacteriol 174: 6685-6687.
3. Collado-Vides, J., Magasanik, B., and Gralla, J.D. (1991) Control site location and transcriptional regulation in Escherichia coli. Microbiol Rev 55: 371-394.
4. Dahl, J.L., Wei, B.Y., and Kadner, R.J. (1997) Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter. J Biol Chem 272: 1910-1919.
5. Drummond, M.H., Contreras, A., and Mitchenall, L.A. (1990) The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae. Mol Microbiol 4: 29-37.
6. Fiedler, U., and Weiss, V. (1995) A common switch in activation of the response regulators NtrC and PhoB: phosphorylation induces dimerization of the receiver modules. EMBO J 14: 3696-3705.
7. Flashner, Y., Weiss, D.S., Keener, J., and Kustu, S. (1995) Constitutive forms of the enhancer-binding protein NtrC: evidence that essential oligomerization determinants lie in the central activation domain. J Mol Biol 249: 700-713.
8. Gu, B., Lee, J.H., Hoover, T.R., Scholl, D., and Nixon, B.T. (1994) Rhizobium meliloti DctD, a σ-dependent transcriptional activator, may be negatively controlled by a subdomain in the C-terminal end of its two-component receiver module. Mol Microbiol 13: 51-66.
9. Ireton, K., Rudner, D.Z., Siranosian, K.J., and Grossman, A.D. (1993) Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor. Genes Dev 7: 283-294.
10. Island, M.D., Wei, B.-Y., and Kadner, R.J. (1992) Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium. J Bacteriol 174: 2754-2762.
11. Kahn, D., and Ditta, G. (1991) Modular structure of FixJ: homology of the transcriptional activator domain with the -35 binding domain of sigma factors. Mol Microbiol 5: 987-997.
12. Klose, K.E., Weiss, D.S., and Kustu, S. (1993) Glutamate at the site of phosphorylation of nitrogen-regulatory protein NtrC mimics aspartyl-phosphate and activates the protein. J Mol Biol 232: 67-78.
13. Li, J., Kustu, S., and Stewart, V. (1994) In vitro interaction of nitrate-responsive regulatory protein NarL with DNA target sequences in the fdnG, narG, narK, and frdA operon control regions of Escherichia coli K-12. J Mol Biol 241: 150-165.
14. Lowry, D.F., Roth, A.F., Rupert, P.B., Dahlquist, F.W., Moy, F.J., Domaille, P.J., and Matsumura, P. (1994) Signal transduction in chemotaxis. A propagating conformation change upon phosphorylation of CheY. J Biol Chem 269: 26358-26362.
15. Makino, K., Amemura, M., Kim, S.-K., Nakata, A., and Shinagawa, H. (1994) Mechanism of transcription activation of the phosphate regulon in Escherichia coli. In Phosphate in Microorganisms. Cellular and Molecular Biology. Torriani-Gorini, A., Yagil, E., and Silver, S. (eds). Washington, DC: American Society for Microbiology Press, pp. 5-12.
16. Merkel, T.J., Nelson, D.M., Brauer, C.L., and Kadner, R.J. (1992) Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene. J Bacteriol 174: 2763-2770.
17. Nakashima, K., Kanamaru, K., Aiba, H., and Mizuno, T. (1991) Osmoregulatory expression of the porin genes in Escherichia coli: evidence for signal titration in the signal transduction through EnvZ-OmpR phosphotransfer. FEMS Microbiol Lett 66: 43-47.
18. Pao, G.M., Tam, R., Lipshitz, L.S., and Saier, Jr, M.H. (1994) Response regulators: structure, function and evolution. Res Microbiol 145: 356-362.
19. Roman, S.J., Meyers, M., Volz, K., and Matsumura, P. (1992) A chemotactic signaling surface on CheY defined by suppressors of flagellar switch mutations. J Bacteriol 174: 6247-6255.
20. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
21. Sanna, M.G., Swanson, R.V., Bourret, R.B., and Simon, M.I. (1995) Mutations in the chemotactic response regulator, CheY, that confer resistance to the phosphatase activity of CheY. Mol Microbiol 15: 1069-1079.
22. Schagger, H., and Von Jagow, G. (1987) Tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100kDa. Anal Biochem 166: 368-379.
23. Shukla, D., and Matsumura, P. (1995) Mutations leading to altered CheA binding cluster on a face of CheY. J Biol Chem 270: 24414-24419.
24. Stock, A.M., Mottonen, J.M., Stock, J.B., and Schutt, C.E. (1989a) Three-dimensional structure of CheY, the response regulator of bacterial chemotaxis. Nature 337: 745-749.
25. Stock, J.B., Ninfa, A.J., and Stock, A.M. (1989b) Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev 53: 450-490.
26. Tsuzuki, M., Aiba, H., and Mizuno, T. (1994) Gene activation by the Escherichia coli positive regulator OmpR. Phosphorylation-independent mechanism of activation by an OmpR mutant. J Mol Biol 242: 607-613.
27. Volz, K., and Matsumura, P. (1991) Crystal structure of Escherichia coli CheY refined at 1.7Å resolution. J Biol Chem 266: 15511-15519.
28. Webber, C.A., and Kadner, R.J. (1995) Action of receiver and activator modules of UhpA in transcriptional control of the Escherichia coli sugar phosphate transport system. Mol Microbiol 15: 883-893.
29. Weston, L.A., and Kadner, R.J. (1988) Role of uhp genes in expression of the Escherichia coli sugar-phosphate transport system. J Bacteriol 170: 3375-3383.