Expression of natural and synthetic genes encoding herpes simplex virus 1 protease in Escherichia coli and purification of the protein

European Journal of Biochemistry

Volumn 247, Issue 3, 1997, Pages 890-895

  Apeler, Heiner ^a   Gottschalk, Uwe ^a   Guntermann, Diana ^a   Hansen, Jutta ^a   Mässen, Jutta ^a   Schmidt, Elisabeth ^a   Schneider, Karl Heinz ^a   Schneidereit, Marcus ^a   Rübsamen Waigmann, Helga ^a  

^a BAYER AG   (Germany)

Author keywords

Herpes simplex virus 1 protease; Protein expression; Protein purification; Synthetic gene

Indexed keywords

PROTEINASE; SYNTHETIC DNA; VIRUS ENZYME;

ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE CONSTRUCT; GENE TRANSFER; HERPES SIMPLEX VIRUS 1; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TECHNIQUE;

ESCHERICHIA COLI; HERPES; HUMAN HERPESVIRUS 1;

EID: 0030796856     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00890.x     Document Type: Article

References (19)

1. McGeoch, D. J., Dalrymphe, M. A., Davison, A. J., Dolan, A., Frame, M. C., McNab, D., Perry, L. J., Scott, J. E. & Taylor, E. (1988) The complete DNA-sequence of the long unique region in the genome of herpes simplex virus type 1, J. Gen. Virol. 69, 1531-1574.
2. Whitley, R. J. (1990) Herpes simplex viruses, in Virology (Fields, B. N., ed.) pp. 1843-1887, Raven press, New York.
3. Schneweis, K. E. & Eis-Hübinger, A. M. (1992) Klinik und Therapie der Infektionen mit Herpes-Simplex-Viren, Internist 33, 581-585.
4. Liu, F. & Roizmann, B. (1991) The promoter transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame, J. Virol. 65, 5149-5156.
5. Preston, V. G., Coates, J. A. & Rixon, F. J. (1983) Identification and characterization of a herpes simplex gene product required for encapsidation of the viral DNA, J. Virol. 45, 1056-1064.
6. Gao, M., Matusick-Kumar, L., Hurlburt, W., Feuer DiTusa, S., Newcomb, W. W., Brown, J. C., McCann, P. J. III, Dechmann, I. C. & Colonno, R. J. (1994) The protease of herpes simplex virus type 1 is essential for functional capsid formation and viral growth, J. Virol. 68, 3702-3712.
7. Matusick-Kumar, L., McCann, P. J. III, Robertson, B. J., Newcomb, W. W., Brown, J. C. & Gao, M. (1995) Release of the catalytic domain No from the herpes simplex virus type 1 protease is required for viral growth, J. Virol. 69, 7113-7121.
8. Weinheimer, S. P., McCann, P. J. III, O'Boyle, D. R. II, Stevens, J. T., Boyd, B. A., Drier, D. A., Yamanaka, G. A., Dilanni, C. L., Deckmann, I. C. & Cordingley, M. G. (1993) Autoproteolysis of herpes simplex virus type 1 protease releases an active catalytic domain found in intermediate capsid particles, J. Virol. 67, 5813-5823.
9. DiIanni, C. L., Mapelli, C., Drier, D. A., Tsao, J., Natarajan, S., Riexinger, D., Festin, S. M., Bolgar, M., Yamanaka, G., Weinheimer, S. P., Meyers, C. A., Colonno, R. J. & Cordingley, M. C. (1993) In vitro activity of the herpes simplex virus type 1 protease with peptide substrates, J. Biol. Chem. 268, 25449-25454.
10. Beattie, K. L. & Fowler, R. F. (1991) Solid-phase gene assembly, Nature 352, 548-549.
11. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes, Methods Enzymol. 185, 60-89.
12. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
13. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. & Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid, Anal. Biochem. 150, 76-85.
14. Gill, S. C. & Hippel, P. H. (1989) Calculation of protein extinction coefficients from amino acid sequence data, Anal. Biochem. 182, 319-326.
15. Grosjean, H. & Fiers, W. (1982) Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes, Gene (Amst.) 18, 199-209.
16. Kane, J. F. (1995) Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli, Curr. Opin. Biotechnol. 6, 494-500.
17. Sharp, P. M. & Li, W.-H. (1987) The codon adaptation index - a measure of directional synonymous codon usage bias, and its potential applications, Nucleic Acids Res. 15, 1281-1295.
18. Darke, P. L., Chen, E., Hall, D. L., Sardanna, M. K., Veloski, C. A., LaFemina, R. L., Shafer, J. A. & Kuo, L. C. (1994) Purification of active herpes simplex virus-1 protease expressed in E. coli, J. Biol. Chem. 269, 18708-18711.
19. Martin, S. L., Vrhovski, B. & Weiss, A. S. (1995) Total synthesis and expression in Escherichia coli of a gene encoding human tropoelastin, Gene (Amst.) 154, 159-166.