Crystallization and preliminary X-ray diffraction studies of expressed Pseudomonas putida catechol 2,3-dioxygenase

Journal of Biochemistry

Volumn 122, Issue 1, 1997, Pages 201-204

  Kita, Akiko ^a   Kita, Shin Ichi ^a,b   Inaka, Koji ^b   Ishida, Tetsuo ^c   Horiike, Kihachiro ^c   Nozaki, Mitsuhiro ^c   Miki, Kunio ^a,d  

^a KYOTO UNIVERSITY   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c SHIGA UNIVERSITY OF MEDICAL SCIENCE   (Japan)

^d UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Catechol 2,3 dioxygenase; Crystallization; Metapyrocatechase; Non heme iron dioxygenase; X ray crystallography

Indexed keywords

ALCOHOL; BACTERIAL ENZYME; CATECHOL; CATECHOL 1,2 DIOXYGENASE; CITRATE SODIUM; FERROUS ION; OXYGENASE;

ARTICLE; CRYSTALLIZATION; ENZYME STRUCTURE; ENZYME SUBUNIT; MOLECULAR WEIGHT; NONHUMAN; PSEUDOMONAS PUTIDA; SYNCHROTRON; X RAY DIFFRACTION;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0030795859     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021729     Document Type: Article

References (18)

1. Kojima, Y., Itada, N., and Hayaishi, O. (1961) Metapyrocatechase: a new catechol-cleaving enzyme. J. Biol. Chem. 236, 2223-2228
2. Nakai, C., Hori, K., Kagamiyama, H., Nakazawa, T., and Nozaki, M. (1983) Purification, subunit structure, and partial amino acid sequence of metapyrocatechase. J. Biol. Chem. 258, 2916-2922
3. Nakai, C., Kagamiyama, H., Nozaki, M., Nakazawa, T., Inouye, S., Ebina, Y., and Nakazawa, A. (1983) Complete nucleotide sequence of the metapyrocatechase gene on the TOL plasmid of Pseudomonas putida mt-2. J. Biol. Chem. 258, 2923-2928
4. Nozaki, M., Kagamiyama, H., and Hayaishi, O. (1963) Metapyrocatechase. I. Purification, crystallization and some properties. Biochem. Z. 338, 582-590
5. Nozaki, M., Ono, K., Nakagawa, T., Kotani, S., and Hayaishi, O. (1968) Metapyrocatechase. II. The role of iron and sulfhydryl groups. J. Biol. Chem. 243, 2682-2690
6. Kobayashi, T., Ishida, T., Horiike, K., Takahara, Y., Numao, N., Nakazawa, A., Nakazawa, T., and Nozaki, M. (1995) Overexpression of Pseudomonas putida catechol 2,3-dioxygenase with high specific activity by genetically engineered Escherichia coli. J. Biochem. 117, 614-622
7. Ohlendorf, D.H., Lipscomb, J.D., and Weber, P.C. (1988) Structure and assembly of protocatechuate 3,4-dioxygenase. Nature 336, 403-405
8. Ohlendorf, D.H., Orville, A.M., and Lipscomb, J.D. (1994) Structure of protocatechuase 3,4-dioxygenase from Pseudomonas aeruginosa at 2.15 A resolution. J. Mol. Biol. 244, 586-608
9. Boyington, J.C., Gaffney, B.J., and Amzel, L.M. (1993) The three-dimensional structure of an arachidonic acid 15-lipoxygenase. Science 260, 1482-1486
10. Sugiyama, K., Senda, T., Narita, H., Yamamoto, T., Kimbara, K., Fukuda, M., Yano, K., and Mitsui, Y. (1995) Three-dimensional structure of 2,3-dihydroxybiphenyl dioxygenase (BphC enzyme) from Pseudomonas sp. strain KKS102 having polychlorinated biphenyl degrading activity. Proc. Jpn. Acad. 71B, 32-35
11. Senda, T., Sugiyama, K., Narita, H., Yamamoto, T., Kimbara, K., Fukuda, M., Sato, M., Yano, K., and Mitsui, Y. (1996) Three-dimensional structure of free form and two substrate complexes of an extradiol ring-cleavage type dioxygenase, the BphC enzyme from Pseudomonas sp. strain KKS102. J. Mol. Biol. 255, 735-752
12. Han, S., Eltis, L.D., Timmis, K.N., Muchmore, S.W., and Bolin, J.T. (1995) Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad. Science 270, 976-980
13. Otwinowski, Z. (1991) DENZO. "A Film Processing Program for Macromolecular Crystallography." Yale Univ., New Haven, CT
14. Sakabe, N. (1991) X-ray diffraction data collection system for modern protein crystallography with Weissenberg camera and an imaging plate using synchrotron radiation. Nucl. Instrum. Methods A303, 448-463
15. Miyahara, J., Takahashi, K., Amemiya, Y., Kamiya, N., and Satow, Y. (1986) A new type of X-ray area detector utilizing laser stimulated luminescence. Nucl. Instrum. Methods A246, 572-578
16. Higashi, T. (1989) The processing of diffraction data taken on a screenless Weissenberg camera for macromolecular crystallography. J. Appl. Crystallogr. 22, 9-18
17. Steigemann, W. (1992) PROTEIN Version 3.1, A program System for the Crystal Structure Analysis of Proteins. Max-Planck Institute für Biochemie, Martinsried
18. Matthews, B.W. (1968) Solvent content of protein crystals. J. Mol. Biol. 33, 494-497