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Another explanation for the observed solvent effects is that the conformation of the cyclobutane uridine dimer is altered in different media. Although this cannot be ruled out, the descriptive torsion O(4)-C(4)-C(S)-C(5′). which is responsible for the orbital overlap, must change by several degrees in order to influence the cleavage rate by a factor of 4. We believe currently that such a torsion angle change, within a rigid cyclobutane pyrimidine dimer framework, is unlikely.
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