N-ethylmaleimide (NEM) can significantly improve in situ hybridization results using 35S-labeled oligodeoxynucleotide or complementary RNA probes

Journal of Histochemistry and Cytochemistry

Volumn 45, Issue 7, 1997, Pages 1035-1041

  Zoeller, R Thomas ^a,d   Fletcher, Donald L ^a   Butnariu, Olimpia ^b   Lowry, Christopher A ^c   Moore, Frank L ^c  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

^b University of Missouri   (United States)

^c OREGON STATE UNIVERSITY   (United States)

^d Morrill Science Center   (United States)

Author keywords

35S labeled probes; cRNA; In situ hybridization; N ethylmaleimide; Oligodeoxynucleotide

Indexed keywords

N ETHYLMALEIMIDE; OLIGODEOXYNUCLEOTIDE;

ALKYLATION; ANIMAL TISSUE; ARTICLE; CHEMICAL INTERACTION; DISULFIDE BOND; IMMOBILIZED CELL; IN SITU HYBRIDIZATION; LABELING INDEX; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; RNA PROBE; SIGNAL NOISE RATIO; TISSUE DISTRIBUTION;

ANIMALIA;

EID: 0030794225     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549704500712     Document Type: Article

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