Use of Tn5-gusA5 to investigate environmental and nutritional effects on gene expression in the coronatine biosynthetic gene cluster of Pseudomonas syringae pv. glycinea

Canadian Journal of Microbiology

Volumn 43, Issue 6, 1997, Pages 517-525

  Palmer, David A ^a,b   Bender, Carol L ^a   Sharma, Shashi B ^c  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b Zeneca Ag Products   (United States)

^c AGRESEARCH GRASSLANDS   (New Zealand)

Author keywords

Gene expression; Glucuronidase; Reporter gene; Transcriptional fusion

Indexed keywords

AMIDE; AMINO ACID; CARBON; FRUCTOSE; ISOLEUCINE; NITROGEN; PLANT TOXIN; PLASMID DNA; POLYKETIDE; VALINE; XYLOSE;

AGAR GEL ELECTROPHORESIS; ARTICLE; ENVIRONMENT; ESCHERICHIA COLI; GENE; GENE CLUSTER; GENE EXPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; NUTRITION; OPERON; PRIORITY JOURNAL; PSEUDOMONAS SYRINGAE; TEMPERATURE DEPENDENCE;

CELL FUSING AGENT VIRUS; ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS SYRINGAE; PSEUDOMONAS SYRINGAE PV. GLYCINEA;

EID: 0030792886     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/m97-074     Document Type: Article

References (56)

1. Bender, C.L., Stone, H.E., Sims, J.J., and Cooksey, D.A. 1987. Reduced pathogen fitness of Pseudomonas syringae pv. tomato Tn5 mutants defective in coronatine production. Physiol. Mol. Plant Pathol. 30: 273-283.
2. Bender, C.L., Young, S.A., and Mitchell, R.E. 1991. Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae. Appl. Environ. Microbiol. 57: 993-999.
3. Bender, C.L., Liyanage, H., Palmer, D., Ullrich, M., Young, S., and Mitchell, R. 1993. Characterization of the genes controlling the biosynthesis of the polyketide phytotoxin coronatine including conjugation between coronafacic and coronamic acid. Gene, 133: 31-38.
4. Berg, C.M., Berg, D.E., and Groisman, E.A. 1989. Transposable elements and the genetic engineering of bacteria. In Mobile DNA. Edited by D.E. Berg and M.M. Howe. American Society for Microbiology, Washington, D.C. pp. 879-925.
5. Chow, W.-Y., and Berg, D.E. 1988. Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations. Proc. Natl. Acad. Sci. U.S.A. 85: 6468-6472.
6. Crouse, G.F., Frischauf, A., and Lehrach, H. 1983. An integrated and simplified approach to cloning into plasmids and single-stranded phages. Methods Enzymol. 101: 78-89.
7. Cuppels, D.A., and Ainsworth, T. 1995. Molecular and physiological characterization of Pseudomonas syringae pv. tomato and Pseudomonas syringae pv. maculicola strains that produce the phytotoxin coronatine. Appl. Environ. Microbiol. 61: 3530-3536.
8. De Bruijn, F.J., and Lupski, J.R. 1984. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids - a review. Gene, 27: 131-149.
9. Feys, B.J.F., Benedetti, C.E., Penfold, C.N., and Turner, J.G. 1994. Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen. Plant Cell, 6: 751-759.
10. Fuhrmann, M., and Hennecke, H. 1982. Coding properties of cloned nitrogenase structural genes from Rhizobium japonicum. Mol. Gen. Genet. 187: 419-425.
11. Gnanamanickam, S.S., Starratt, A.N., and Ward, E.W.B. 1982. Coronatine production in vitro and in vivo and its relation to symptom development in bacterial blight of soybean. Can. J. Bot. 60: 645-650.
12. Heu, S., and Hutcheson, S.W. 1993. Nucleotide sequence and properties of the hrmA locus associated with the Pseudomonas syringae pv. svringae 61 hrp gene cluster. Mol. Plant-Microbe Interact. 6: 553-564.
13. Hoitink, H.A.J., and Sinden, S.L. 1970. Partial purification and properties of chlorosis inducing toxins of Pseudomonas phaseolicola and Pseudomonas glycinea. Phytopathology, 60: 1236-1237.
14. Huynh, T.V., Dahlbeck, D., and Staskawicz, B.J. 1989. Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity. Science (Washington, D.C.), 245: 1374-1377.
15. Ichihara, A., Shiraishi, K., Sato, H., Sakamura, S., Nishiyama, K., Sakai, R., Furusaki, A., and Matsumoto, T. 1977. The structure of coronatine. J. Am. Chem. Soc. 99: 636-637.
16. Kado, C.I., and Liu, S.-T. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145: 1365-1373.
17. Keane, P.J., Kerr, A., and New, P.B. 1970. Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Aust. J. Biol. Sci. 23: 585-595.
18. Keen, N.T., Tamaki, S., Kobayashi, D., and Trollinger, D. 1988. Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene, 70: 191-197.
19. Kenyon, J.S., and Turner, J.G. 1992. The stimulation of ethylene synthesis in Nicotiana tabacum leaves by the phytotoxin coronatine. Plant Physiol. 100: 219-224.
20. Kidambi, S.P, Sundin, G.W., Palmer, D.A., Chakrabarty, A.M., and Bender, C.L. 1995. Copper as a signal for alginate synthesis in Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 61: 2172-2179.
21. Lindgren, P.B., Frederick, R., Govindarajan, A.G., Panopoulos, N.J., Staskawicz, B.J., and Lindow, S.E. 1989. An ice nucleation reporter gene system: identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. EMBO J. 8: 1291-1301.
22. Liyanage, H., Palmer, D.A., Ullrich, M., and Bender, C.L. 1995a. Characterization and transcriptional analysis of the gene cluster for coronafacic acid, the polyketide component of the phytotoxin coronatine. Appl. Environ. Microbiol. 61: 3843-3848.
23. Liyanage, H., Penfold, C., Turner, J., and Bender, C.L. 1995b. Sequence, expression and transcriptional analysis of the coronafacate ligase-encoding gene required for coronatine biosynthesis by Pseudomonas syringae. Gene, 153: 17-23.
24. Lorang, J.M., and Keen, N.T. 1995. Characterization of avrE from Pseudomonas svringae pv. tomato: a hrp-linked avirulence locus consisting of at least two transcriptional units. Mol. Plant-Microbe Interact. 8: 49-57.
25. Maniatis, T., Fritsch, E.F., and Sambrook, J. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Merrick, M., Filser, M., Kennedy, C., and Dixon, R. 1978. Polarity of mutations induced by insertion of transposons Tn5, Tn7 and Tn10 into the nif gene cluster of Klebsiella pneumoniae. Mol. Gen. Genet. 165: 103-111.
27. Miller, J.H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
28. Mitchell, R.E. 1982. Coronatine production by some phytopathogenic pseudomonads. Physiol. Plant Pathol. 20: 83-89.
29. Mitchell, R.E., and Frey, E.J. 1986. Production of N-coronafacoyl-L-amino acid analogues of coronatine by Pseudomonas syringae pv. atropurpurea in liquid cultures supplemented with L-amino acids. J. Gen. Microbiol. 132: 1503-1507.
30. Mitchell, R.E., and Young, H. 1978. Identification of a chlorosis-inducing toxin of Pseudomonas glycinea as coronatine. Phytochemistry, 17: 2028-2029.
31. Mitchell, R.E., Hale, C.N., and Shanks, J.C. 1983. Production of different pathogenic symptoms and different toxins by strains of Pseudomonas syringae pv. tomato not distinguishable by gel-immunodiffusion assay. Physiol. Plant Pathol. 23: 315-322.
32. Mittal, S., and Davis, K.R. 1995. Role of the phytotoxin coronatine in the infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato. Mol. Plant-Microbe Interact. 8: 165-171.
33. Nishiyama, K., Sakai, R., Ezuka, A., Ichihara, A., Shiraishi, K., Ogasawara, M., Sato, H., and Sakamura, S. 1976. Phytotoxic effect of coronatine produced by Pseudomonas coronafaciens var. atropurpurea on leaves of Italian ryegrass. Ann. Phytopathol. Soc. Jpn. 42: 613-614.
34. Palmer, D.A., and Bender, C.L. 1993. Effects of environmental and nutritional factors on production of the polyketide phytotoxin coronatine by Pseudomonas syringae pv. glycinea. Appl. Environ. Microbiol. 59: 1619-1626.
35. Palmer, D.A., and Bender, C.L. 1995. Ultrastructure of tomato leaf tissue treated with the pseudomonad phytotoxin coronatine and comparison with methyl jasmonate. Mol. Plant-Microbe Interact. 8: 683-692.
36. Pannekoek, H., Hille, J., and Noordermeer, I. 1980. Relief of polarity caused by transposon Tn5: application in mapping a cloned region of the Escherichia coli uvrB locus essential for UV resistance. Gene, 12: 51-61.
37. Parry, R.J., Mhaskar, S.V., Lin, M.-T., Walker, A.E., and Mafoti, R. 1994. Investigations of the biosynthesis of the phytotoxin coronatine. Can. J. Chem. 72: 86-99.
38. Peñaloza-Vázquez, A., Rangaswamy, V., Ullrich, M., Bailey, A.M., and Bender, C.L. 1996. Use of translational fusions to the maltose binding protein to produce and purify proteins in Pseudomonas syringae and assess their activity in vivo. Mol. Plant-Microbe Interact. 9: 637-641.
39. Penfold, C.N., Bender, C.L., and Turner, J.G. 1996. Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine. Gene, 183: 167-173.
40. Rahme, L.G., Mindrinos, M.N., and Panopoulos, N.J. 1992. Plant and environmental sensory signals control the expression of hrp genes in Pseudomonas syringae pv. phaseolicola. J. Bacteriol. 174: 3499-3507.
41. Sakai, R., Nishiyama, K., Ichihara, A., Shiraishi, K., and Sakamura, S. 1979. The relation between bacterial toxic action and plant growth regulation. In Recognition and specificity in plant host-parasite interactions. Edited by J.M. Daly and I. Uritani. University Park Press, Baltimore, Md. pp. 165-179.
42. Salmeron, J.M., and Staskawicz, B.J. 1993. Molecular characterization and hrp dependence of the avirulence gene avrPto from Pseudomonas syringae pv. tomato. Mol. Gen. Genet. 239: 6-16.
43. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
44. Sasakawa, O., and Yoshikawa, M. 1987. A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis. Gene, 56: 286-288.
45. Sato, M. 1988. In planta transfer of the genes for virulence between isolates of Pseudomonas syringae pv. atropurpurea. Ann. Phytopathol. Soc. Jpn. 54: 20-24.
46. Shanahan, P., O'Sullivan, D.J., Simpson, P., Glennon, J.D., and O'Gara, F. 1992. Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production. Appl. Environ. Microbiol. 58: 353-358.
47. Sharma, S.B., and Signer, E.R. 1990. Temporal and spatial regulation of the symbiotic genes of Rhizobium meliloti in planta revealed by transposon Tn5-gusA. Genes Dev. 4: 344-356.
48. Slininger, P.J., and Shea-Wilbur, M.A. 1995. Liquid-culture pH, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent Pseudomonas fluorescens 2-79. Appl. Microbiol. Biotechnol. 43: 794-800.
49. Stachel, S.E., An, G., Flores, C., and Nester, E.W. 1985. A Tn3 lacZ transposon for the random generation of β-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 4: 891-898.
50. Ullrich, M., and Bender, C.L. 1994. The biosynthetic gene cluster for coronamic acid, an ethylcyclopropyl amino acid, contains genes homologous to amino acid-activating enzymes and thioesterases. J. Bacteriol. 176: 7574-7586.
51. Ullrich, M., Guenzi, A.C., Mitchell, R.E., and Bender, C.L. 1994. Cloning and expression of genes required for coronamic acid (2-ethyl-1-aminocyclopropane 1-carboxylic acid), an intermediate in the biosynthesis of the phytotoxin coronatine. Appl. Environ. Microbiol. 60: 2890-2897.
52. Ullrich, M., Peñaloza-Vázquez, A., Bailey, A.M., and Bender, C.L. 1995. A modified two-component regulatory system is involved in the temperature-dependent biosynthesis of the Pseudomonas syringae phytotoxin coronatine. J. Bacteriol. 177: 6160-6169.
53. Wilmes-Riesenberg, M.R., and Wanner, B.L. 1992. TnphoA and TnphoA' elements for making and switching fusions for study of transcription, translation, and cell surface localization. J. Bacteriol. 174: 4558-4575.
54. Xiao, Y., Lu, Y., Heu, S., and Hutcheson, S.W. 1992. Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster. J. Bacteriol. 174: 1734-1741.
55. Young, S.A., Park, S.K., Rodgers, C., Mitchell, R.E., and Bender, C.L. 1992. Physical and functional characterization of the gene cluster encoding the polyketide phytotoxin coronatine in Pseudomonas syringae pv. glycinea. J. Bacteriol. 174: 1837-1843.
56. Zhang, Y., Rowley, K.B., and Patil, S.S. 1993. Genetic organization of a cluster of genes involved in the production of phaseolotoxin, a toxin produced by Pseudomonas syringae pv. phaseolicola. J. Bacteriol. 175: 6451-6458.