Excretion of dissolved organic nitrogen by phytoplankton assessed by wet oxidation and 15N tracer procedures

Marine Ecology Progress Series

Volumn 153, Issue 1-3, 1997, Pages 99-111

  Pujo Pay, Mireille ^a   Conan, Pascal ^a   Raimbault, Patrick ^a  

^a STATION MARINE D ENDOUME   (France)

Author keywords

15N methodology; Dissolved organic nitrogen; Phytoplankton excretion; Wet oxidation

Indexed keywords

DIATOM; DISSOLVED ORGANIC NITROGEN; GREEN ALGAE; NITROGEN EXCRETION; PHYTOPLANKTON;

ALGAE; BACILLARIOPHYTA; BACTERIA (MICROORGANISMS); CHLOROPHYCEAE; DUNALIELLA TERTIOLECTA; PHAEODACTYLUM TRICORNUTUM;

EID: 0030792350     PISSN: 01718630     EISSN: None     Source Type: Journal    
DOI: 10.3354/meps153099     Document Type: Article

References (93)

1. Aaronson S (1978) Excretion of organic matter by phytoplankton in vitro. Limnol Oceanogr 23:838
2. Andersen OK, Goldman JC, Caron DA, Dennett MR (1986) Nutrient cycling in a microflagellate food chain: III. Phosphorus dynamics. Mar Ecol Prog Ser 31:47-55
3. Andersson A, Lee C, Azam F, Hagström Å (1985) Release of amino acids and inorganic nutrients by heterotrophic marine microflagellates. Mar Ecol Prog Ser 23:99-106
4. Antia NJ, Harrison PJ, Oliveira L (1991) The role of dissolved organic nitrogen in phytoplankton nutrition, cell biology and ecology. Phycologia 30:1-89
5. 14 method of measuring primary productivity. Limnol Oceanogr 12:121-214
6. Azam F, Cho BC, Smith DC, Simon M (1990) Bacterial cycling of matter in the pelagic zone of aquatic ecosystem. In: Tilzer MM, Serruya C (eds) Large lakes - ecological structure and function. Springer-Verlag, Berlin, p 477-488
7. Azam F, Fenchel T, Field JG, Gray JS, Meyer-Reil LA, Thingstad F (1983) The ecological role of water-column microbes in the sea. Mar Ecol Prog Ser 10:257-263
8. Baines SB, Paces ML (1991) The production of dissolved organic matter by phytoplankton and its importance to bacteria: patterns accoss marine and freshwater systems. Limnol Oceanogr 36:1078-1090
9. Berland BR, Bonin DJ, Maestrini SY (1980) Azote ou phosphore? Considérations sur le 'paradoxe nutritionnel' de la mer Méditerranée. Oceanologica Acta 3:135-142
10. Bohlin K (1897) Zur Morphologie und Biologie einzelliger Algen. Öfvers Förh Kongl Svenska Vetensk-Akad 9: 507-529
11. Bonin (1988) Role du phosphore organique dissous dans la production primaire. Oceanis 14(2):381-387
12. Bratbak G, Heldal M, Thingstad TF, Riemann B, Haslund OH (1992) Incorporation of viruses into the budget of microbial C-transfer. A first approach. Mar Ecol Prog Ser 86: 273-280
13. 15N tracer method for the measurement of dissolved organic nitrogen release by phytoplankton. Mar Ecol Prog Ser 77:171-182
14. 15N tracer method to the study of dissolved organic nitrogen uptake during spring and summer in Chesapeake Bay. Mar Biol 115:501-508
15. 15N differs among marine systems. Limnol Oceanogr 39(1): 189-195
16. Bronk DA, Glibert PM, Ward BB (1994) Nitrogen uptake, dissolved organic nitrogen release, and new production. Science 265:1843-1846
17. Butcher RW (1952) Contributions to our knowledge of the smaller marine algae. J Mar Biol Assoc UK 31:175-191
18. Chan YK, Campbell NER (1978) Phytoplankton uptake and excretion of assimilate nitrate in a small Canadian shield lake. Appl Environ Microbiol 35:1052-1060
19. Codispoti LA (1989) Phosphorus versus nitrogen limitation of new and export production. In: Berger WH, Smetacek VS, Wefer G (eds) Productivity of the ocean: present and past. J Wiley and Sons, Chichester, p 377-394
20. Cole JJ, Likens GE, Strayer DL (1982) Photosynthetically produced dissolved organic carbon: an important source for planktonic bacteria. Limnol Oceanogr 27:1080-1090
21. Collos Y (1986) Time-lag algal growth dynamics: biological constraints on primary production in aquatic environments. Mar Ecol Prog Ser 33:193-206
22. Collos Y (1992) Nitrogen budgets and dissolved organic matter cycling. Mar Ecol Prog Ser 90:201-206
23. Collos Y, Döhler G, Biermann I (1992) Production of dissolved organic nitrogen during uptake of nitrate by Synedra planctonica: implications for estimates of new production in the oceans. J Plankton Res 14:1025-1029
24. Corner EDS, Davies AG (1971) Plankton as a factor in the nitrogen and phosphorus cycles in the sea. Adv Mar Biol 9: 101-204
25. Droop MR (1974) The nutrient status of algal cells in continuous culture. J Mar Biol Assoc UK 54:825-855
26. Duarte CM (1990) Time-lag in algal growth: generality, causes and consequences. J Plankton Res 12:873-883
27. Eppley RW, Peterson BJ (1979) Particulate organic matter and planktonic new production in deep ocean. Nature 282: 311-323
28. Eppley RW, Renger EH, Venrick EL, Mullin MN (1973) A study of plankton dynamics and nutrient cycling in the central gyre of the North Pacific Ocean. Limnol Oceanogr 18:534-551
29. Fogg GE (1965) Algal culture and phytoplankton ecology. University of Wisconsin Press, Madison
30. Fogg GE (1977) Excretion of organic matter by phytoplankton. Limnol Oceanogr 22:576-577
31. Fogg GE (1983) The ecological significance of extracellular products of phytoplankton photosynthesis. Bot Mar 26: 3-14
32. Fogg GE, Nalewajko G, Watt WD (1965) Extracellular products of phytoplankton photosynthesis. Proc R Soc Lond B 162:517-534
33. Fuhrman J (1987) Close coupling between release and uptake of dissolved free amino acids in seawater studied by an isotope dilution approach. Mar Ecol Prog Ser 37:45-52
34. Fuhrman JA, Bell TM (1985) Biological considerations in the measurement of dissolved free amino acids in seawater and implications for chemical and microbiological studies. Mar Ecol Prog Ser 25:13-21
35. Glibert PM, Bronk DA (1994) Release of dissolved organic nitrogen by marine diazotrophic cyanobacteria, Trichodesmium spp. Appl Environ Microbiol 60(11):3996-4000
36. Goldman JC, Caron DA, Andersen OK, Dennett MR (1985) Nutrient cycling in a microflagellate food chain: I. Nitrogen dynamics. Mar Ecol Prog Ser 24:231-242
37. Goldman JC, Dennett MR (1985) Susceptibility of some marine phytoplankton species to cell breakage during filtration and post-filtration rinsing. J Exp Mar Biol Ecol 86: 47-58
38. Goldman JC, McCarthy JJ, Peavey DG (1979) Growth rate influence on the chemical composition of phytoplankton in oceanic waters. Nature 279:210-215
39. Grenney WJ, Bella DA, Curl HC (1973) A theoretical approach to interspecific competition in phytoplankton communities. Am Nat 107:405-425
40. Guillard RRL, Ryther JH (1962) Studies of marine planktonic diatoms. I. Cyclotella nana (Hustedt) and Detonula confervacea (Cleve) Gran. Can J Microbiol 8:229-239
41. 15C]urea. Can J Fish Aquat Sci 46:198-202
42. Harrison PJ, Conway HL, Dugdale RC (1976) Marine diatoms grown in chemostats under silicate or ammonium limitation. I. Cellular chemical composition and steady-state growth kinetics of Skeletonema costatum. Mar Biol 35: 177-186
43. Harrison PJ, Conway HL, Holmes RW, Davis CO (1977) Marine diatoms in chemostats under silicate or ammonium limitation. III. Cellular chemical composition and morphology of Chaetoceros debilis, Skeletonema costatum, and Thalassiosira gravida. Mar Biol 43:19-31
44. Hedges JI, Bergamaschi BA, Benner R (1993) Comparative analyses of DOC and DON in natural waters. Mar Chem 41:121-134
45. Herbland A (1974) Influence de la dépression de filtration sur la mesure simultanée de l'assimilation et de l'excrétion organique du phytoplancton. Cah ORSTOM Sér Océanogr 12:173-174
46. Howarth RW, Cole JJ (1985) Molybdenum availability, nitrogen limitation, and phytoplankton growth in natural waters. Science 229:653-655
47. Jensen LM (1983) Phytoplankton release of extracellular organic carbon, molecular weight composition, and bacterial assimilation. Mar Ecol Prog Ser 11:39-48
48. Jørgensen NOG, Kroer N, Coffin RB, Yang XH, Lee C (1993) Dissolved free amino acids, combined amino acids, and DNA as sources of carbon and nitrogen to marine bacteria. Mar Ecol Prog Ser 98:135-148
49. Kirchman DL, Ducklow HW, McCarthy JJ, Garside C (1994) Biomass and nitrogen uptake by heterotrophic bacteria during the spring phytoplanktonic bloom in the North Atlantic Ocean. Deep Sea Res 41(5-6):879-895
50. Kirchman DL, Moss J, Keil RG (1992) Nitrate uptake by heterotrophic bacteria: does it change the f-ratio? Arch Hydrobiol Beih Ergeb Limnol 37:129-138
51. Kirchman DL, Suzuki Y, Garside C, Ducklow HW (1991) High turnover rates of dissolved organic carbon during a spring phytoplankton bloom. Nature 352:612-614
52. Kofoid CA (1903) The plankton of the Illinois River, 1894-1899, with introductory notes upon the hydrography of the Illinois River and its basin. Part 1. Quantitative investigations and general results. Bull Illinois State Lab Nat Hist 6:95-629
53. Koroleff F (1969) Direct determination of ammonia in natural waters as indophenol blue. Comm Meet Int Counc Explor Sea CM-ICES/C:9
54. Lancelot C (1983) Factors affecting phytoplankton extracellular release in the southern bight of the North Sea. Mar Ecol Prog Ser 12:115-121
55. 15N. Limnol Oceanogr 29:379-386
56. Li WKW (1990) Particles in 'particles-free' seawater growth of ultraphytoplankton and implications for dilution experiments. Can J Fish Aquat Sci 47:1258-1268
57. Malinsky-Rushansky NZ, Legrand C (1996) Excretion of dissolved organic carbon by phytoplankton of different sizes and subsequent bacterial uptake. Mar Ecol Prog Ser 132: 249-255
58. McCarthy JJ, Taylor WR, Taft JL (1977) Nitrogenous nutrition of the plankton in the Chesapeake Bay. I. Nutrient availability and phytoplankton preferences. Limnol Oceanogr 22:996-1011
59. Myklestad S (1977) Production of carbohydrates by marine planktonic diatoms. II. Influence of the N/P ratio in the growth medium on the assimilation ratio, growth rate, and production of cellular and extracellular carbohydrates by Chaetoceros affinis var. willei (Gran) Hustedt and Skeletonema costatum (Grev) Cleve. J Exp Mar Biol Ecol 29: 161-179
60. Nagata T, Kirchman DL (1991) Release of dissolved free and combined amino acids by bacterivorous marine flagellates. Limnol Oceanogr 36:433-443
61. Nalewajko C, Schindler DW (1976) Primary production, extracellular release, and heterotrophy in two lakes in the ELA, northwestern Ontario. J Fish Res Bd Can 33: 219-226
62. Newell BS Dalpont G, Grant BR (1972) The excretion of organic nitrogen by marine algae in batch and continuous culture. Can J Bot 50:2605-2611
63. Nyholm N (1977) Kinetics of nitrogen-limited algal growth. Prog Wat Technol 8:347-358
64. Nyholm N, Lyngby JE (1988) Algal bioassays in eutrophication research-discussion in the framework of a mathematical analysis. Water Res 22:1293-1300
65. Obernosterer I, Herndl GJ (1995) Phytoplankton extracellular release and bacterial growth: dependence on the inorganic N/P ratio. Mar Ecol Prog Ser 116:247-257
66. Parsons TR, Stephens K, Strickland JDH (1961) On the chemical composition of eleven species of marine phytopankters. J Fish Res Bd Can 18:1001-1016
67. Parsons TR, Takahashi M, Hargave B (1984) Biological oceanic processes, 3rd edn. Pergamon Press, Oxford
68. Paul JH, Jeffrey WH, DeFlaun MF (1987) Dynamics of extracellular DNA in the marine environment. Appl Environ Microbiol 53:170-179
69. Perry MJ (1976) Phosphate utilization by an oceanic diatom in phosphorus-limited chemostat culture and in the oligotrophic waters of the central north Pacific. Limnol Oceanogr 21:88-107
70. Pujo-Pay M (1995) L'azote et le phosphore en milieu marin. Importance des formes organiques dissoutes en milieu océanique du large. Thèse Doct Univ Aix-Marseille II
71. Pujo-Pay M, Raimbault P (1994) Improvement of the wet-oxidation procedure for similtaneous determination of particulate organic nitrogen and phosphorus collected on filters. Mar Ecol Prog Ser 105:203-207
72. Redfield AC (1958) The biological control of the chemical factors in the environment. Am Sci 46:205-222
73. Ryther JH, Dunstan WM (1971) Nitrogen, phosphorus and eutrophication in the coastal marine environment. Science 171:1008-1013
74. Sharp JH (1977) Excretion of organic matter by marine phytoplankton: do healthy cells do it? Limnol Oceanogr 22: 281-299
75. Sharp JH (1983) The distributions of inorganic nitrogen and dissolved and particulate organic nitrogen in the sea. In: Carpenter EJ, Capone DG (eds) Nitrogen in the marine environment. Academic Press, New York, p 1-35
76. Sharp JH (1984) Inputs into microbial food chains. In: Hobbie JE, Williams PJ LeB (eds) Heterotrophic actinity in the sea. NATO Conferences Series IV, Plenum Press, New York, p 101-120
77. Sharp JH (1993) The dissolved organic carbon controversy: an update. Oceanography 6:45-50
78. 15N-tracer experiments on oceanic waters improving mass balance. Mar Ecol Prog Ser 124: 289-299
79. Slawyk G, Raimbault P, Gentilhomme V (1990) On the discrepancies between a colorimetric and isotopic method for measuring nitrate utilisation in nitrate-depleted waters: implication for the design of experimental protocols in new production stdies. Hydrobiol 207:333-339
80. Stockner JG, Klut ME, Cochlan WP (1990) Leaky filters: a warning to aquatic ecologists. Can J Fish Aquat Sci 47:16-23
81. Suttle CA, Chan AM, Cottrell MT (1990) Infection of phytoplankton by viruses and reduction of primary production. Nature 347:467-469
82. Thingstad TF (1993) Microbial processes and the biological carbon pump. In: Evans GT, Fasham MJR (eds) Towards a model of ocean biogeochemical processes. Springer-Verlag, Berlin, p 193-208
83. Thingstad TF, Rassoulzadegan F (1995) Nutrient limitations, microbial food webs, and 'biological C-pumps': suggested interactions in a P-limited Mediterranean. Mar Ecol Prog Ser 117:299-306
84. Thomas WH (1966) Surface nitrogenous nutrients and phytoplankton in the northeastern tropical Pacific Ocean. Limnol Oceanogr 11:393-400
85. Tréguer P, Le Corre P (1975) Manuel d'analyses des sels nutritifs dans l'eau de mer. Laboratoire d' Océanographie Chimique. Université de Bretagne Occidentale, Brest
86. Turley CM (1985) Biological studies in the vicinity of a shallow-sea tidal mixing front IV. Seasonal and spatial distribution of urea and its uptake by phytoplankton. Phil Trans R Soc Lond B 310:471-500
87. Turley CM (1986) Urea uptake by phytoplankton at different fronts and associated stratified and mixed waters on the european shelf. Br Phycol J 21:225-238
88. Van Wambeke F (1988) Numération et taille des bactéries planctoniques au moyen de l'analyse d'images couplée à l'épifluorescence. Ann Inst Pasteur Microbiol 139:261-272
89. Watanabe Y (1980) A study of the excretion and extracellular products of natural phytoplankton in Lake Nakanuma, Japan. Int Rev Ges Hydrobiol 65:809-834
90. Wheeler PA, Kirchman DL (1986) Utilization of inorganic and organic nitrogen by bacteria in marine system. Limnol Oceanogr 31:998-1009
91. Wood AM, Rai H, Gamier J, Kairesalo T, Gresens S, Orive E, Ravail B (1992) Practical approaches to algal excretion. Mar Microb Food Webs 6:21-38
92. Wood AM, Van Valen LM (1990) Paradox lost? On the excretion of energy-rich compounds by phytoplankton. Mar Microb Food Webs 4:103-116
93. Yentsch CS, Vaccaro RF (1958) Phytoplankton nitrogen in the oceans. Limnol Oceanogr 3:443-448