Negative autoregulation of the Rhizobium meliloti fixK gene is indirect and requires a newly identified regulator, FixT

Molecular Microbiology

Volumn 25, Issue 1, 1997, Pages 27-37

  Foussard, Marie ^a   Garnerone, Anne Marie ^a   Ni, Fudi ^a,b   Soupène, Eric ^a,c   Boistard, Pierre ^a   Batut, Jacques ^a  

^a CNRS   (France)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOREGULATION; BACTERIAL GENETICS; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; REGULATOR GENE; SINORHIZOBIUM MELILOTI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; RHIZOBIUM; SINORHIZOBIUM MELILOTI;

EID: 0030792236     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.4501814.x     Document Type: Article

References (31)

1. Agron, P.G., Ditta, G.S., and Helinski, D.R. (1993) Oxygen regulation of nifA transcription in vitro. Proc Natl Acad Sci USA 90: 3506-3510.
2. Batut, J., Daveran-Mingot, M.L., David, M., Jacobs, J., Garnerone, A.M., and Kahn, D. (1989) fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti. EMBO J 8: 1279-1286.
3. Batut, J., Santero, E., and Kustu, S. (1991) In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti. J Bacteriol 173: 5914-5917.
4. Cherfils, J., Gibrat, J.F., Levin, J., Batut, J., and Kahn, D. (1989) Model-building of Fnr and FixK DNA-binding domains suggest a basis for specific DNA recognition. J Mol Recog 2: 114-121.
5. Conover, W.J. (1980) Practical Non-parametric Statistics. 2nd edn. New York: Wiley and Son.
6. David, M., Daveran, M.L., Batut, J., Dedieu, A., Domergue, O., Ghai, J., Hertig, C., Boistard, P., and Kahn, D. (1988) Cascade regulation of nif gene expression in Rhizobium meliloti. Cell 54: 671-683.
7. de Philip, P., Soupène, E., Batul, J, and Boistard, P. (1992) Modular structure of the FixL protein of Rhizobium meliloti. Mol Gen Genet 235: 49-54.
8. Ditta, G., Schmidhauser, T., Yacobson, E., Lu, P., Liang, X.W., Finlay, D.R., Guiney, D., and Helinski, D.R. (1985) Plasmids related to the broad host range vector, pK290, useful for gene cloning and for monitoring gene expression. Plasmid 13: 149-153.
9. Fischer, H.M. (1994) Genetic regulation of nitrogen fixation in rhizobia. Microbiol Rev 58: 352-386.
10. Galinier, A., Garnerone, A.M., Reyrat, J.M., Kahn, D., Batut, J., and Boistard, P. (1994) Phosphorylation of the Rhizobium meliloti FixJ protein induces its binding to a compound regulatory region at the fixK promoter. J Biol Chem 269: 23784-23789.
11. Gilles-Gonzalez, M.A., Ditta, G.S., and Helinski, D.R. (1991) A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti. Nature 350: 170-172.
12. Gold, L. (1988) Posttranscriptional regulatory mechanisms in Escherichia coli. Annu Rev Biochem 57: 199-233.
13. Green, J., Bennett, B., Jordan, P., Ralph, E.T., Thomson, A.J., and Guest, J.R. (1996) Reconstitution of the [4Fe-4S]cluster in FNR and demonstration of the aerobic-anaerobic transcription switch in vitro. Biochem J 316: 887-892.
14. Hanamura, A., and Aiba, H. (1991) Molecular mechanisms of negative autoregulation of Escherichia coli crp gene. Nucleic Acids Res 19: 4413-4419.
15. Labes, M., Pühler, A., and Simon, R. (1990) A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for Gram-negative bacteria. Gene 89: 37-46.
16. Martin, M.O., and Long, S.R. (1984) Generalized transduction in Rhizobium meliloti. J Bacteriol 159: 125-129.
17. Melville, S.B., and Gunsalus, R.P. (1996) Isolation of an oxygen-sensitive FNR protein of Escherichia coli: Interaction at activator and repressor sites of FNR-controlled genes. Proc Natl Acad Sci USA 93: 1226-1231.
18. Niel, C., Guillaume, B., and Bechet, M. (1977) Mise en évidence de deux enzymes présentant une activité β-galactosidasique chez Rhizobium meliloti. Can J Microbiol 23: 1178-1181.
19. Prentki, P., and Krisch, M. (1984) In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29: 303-313.
20. Preisig, O., Anthamatten, D., and Hennecke, H. (1993) Genes for microaerobically induced oxidase complex in Bradyrhizobium japonicum are essential for a nitrogen-fixing endosymbiosis. Proc Natl Acad Sci USA 90: 3309-3113.
21. Quandt, J., and Hynes, M.F. (1993) Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 12: 15-21.
22. Renalier, M.H., Batut, J., Ghai, J., Terzaghi, B., Ghérardi, M., David, M., Garnerone, A.M., Vasse, J., Truchet, G., Huguet, T., and Boistard, P. (1987) A new symbiotic cluster on the pSym megaplasmid of Rhizobium meliloti 2011 carries a functional fix gene repeat and a nod locus. J Bacteriol 169: 2231-2238.
23. Reyrat, J.M., David, M., Blonski, C., Boistard, P., and Batut, J. (1993) Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes. J Bacteriol 175: 6867-6872.
24. Ringquist, S., Shinedling, S., Barrick, D., Green, L., Binkley, J., Stormo, G.D., and Gold, L. (1992) Translation initiation in Escherichia coli: sequences within the ribosome-binding site. Mol Microbiol 6: 1219-1229.
25. Sharrocks, A.D., Green, J., and Guest, J.R. (1991) FNR activates and represses transcription in vitro. Proc R Soc London B 245: 219-226.
26. 2-fixation gene expression within the alfalfa root nodule. Proc Natl Acad Sci USA 92: 3759-3763.
27. Spiro, S. (1994) The FNR family of transcriptional regulators. Anton van Leeuwenhoek 66: 23-36.
28. Spiro, S., and Guest, J.R. (1990) FNR and its role in oxygen-regulated gene expression in Escherichia coli. FEMS Microbiol Rev 75: 399-428.
29. Ullman, A. (1984) One-step purification of hybrid proteins which have β-galactosidase activity. Gene 29: 27-31.
30. Waelkens, F., Foglia, A., Morel, J.B., Fourment, J., Batut, J., and Boistard, P. (1992) Molecular genetic analysis of the Rhizobium meliloti fixK promoter: identification of sequences involved in positive and negative regulation. Mol Microbiol 6: 1447-1456.
31. Wing, H.J., Williams, S.M., and Busby, S.J.W. (1995) Spacing requirements for transcription activation by Escherichia coli FNR protein. J Bacteriol 177: 6704-6710.