Unique regulation of crystal protein production in Bacillus thuringiensis subsp. yunnanensis is mediated by the Cry protein-encoding 103- megadalton plasmid

Applied and Environmental Microbiology

Volumn 63, Issue 7, 1997, Pages 2792-2797

  Srinivas, G ^a   Vennison, S John ^a   Sudha, S N ^a   Balasubramanian, P ^a   Sekar, Vaithilingam ^a  

^a MADURAI KAMARAJ UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; BACILLUS THURINGIENSIS; BACTERIUM CULTURE; CELL INCLUSION; CRYSTAL STRUCTURE; GENE EXPRESSION REGULATION; GENETIC TRANSDUCTION; NONHUMAN; PLASMID; PROTEIN SYNTHESIS; SOIL MICROFLORA; SPOROGENESIS;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR KURSTAKI; BACILLUS THURINGIENSIS SEROVAR YUNNANENSIS; BACTERIA (MICROORGANISMS); DIPTERA;

EID: 0030790401     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.7.2792-2797.1997     Document Type: Article

References (31)

1. Agaisse, H., and D. Lereclus. 1994. Structural and functional analysis of the promoter region involved in full expression of the cryIII toxin gene of Bacillus thuringiensis. Mol. Microbiol. 13:97-107.
2. Bora, R. S., M. G. Murty, R. Shenbagarathai, and V. Sekar. 1994. Introduction of a lepidopteran-specific insecticidal crystal protein gene of Bacillus thuringiensis subsp. kurstaki by conjugal transfer into a Bacillus megaterium strain that persists in the cotton phyllosphere. Appl. Environ. Microbiol. 60:214-222.
3. Brown, K. L., and H. R. Whiteley. 1988. Isolation of a Bacillus thuringiensis RNA polymerase capable of transcribing a crystal protein gene. Proc. Natl. Acad. Sci. USA 85:4166-4170.
4. Carlton, B. C., and J. M. Gonzalez. 1985. Plasmids and delta-endotoxin production in different subspecies of Bacillus thuringiensis, p. 246-252. In J. A. Hoch and P. Setlow (ed.), Molecular biology of microbial differentiation. American Society for Microbiology, Washington, D.C.
5. Chung, J. D., G. Stephanopoulos, K. Ireton, and A. D. Grossman. 1994. Gene expression in single cells of Bacillus subtilis: evidence that a threshold mechanism controls the initiation of sporulation. J. Bacteriol. 176:1977-1984.
6. de Barjac, H. 1981. Identification of H-serotypes of Bacillus thuringiensis, p. 35-13. In H. D. Burges (ed.), Microbial control of pests and plant diseases, 1970-1980. Academic Press, London, England.
7. Fisher, H. M., P. Luthy, and S. Schweitzer. 1984. Introduction of plasmid pC194 in Bacillus thuringiensis by protoplast transformation and plasmid transfer. Arch. Microbiol. 139:213-217.
8. Gonzalez, J. M., B. J. Brown, and B. C. Carlton. 1982. Transfer of Bacillus thuringiensis plasmids coding for delta-endotoxin among strains of Bacillus thuringiensis and Bacillus cereus. Proc. Natl. Acad. Sci. USA 79:6951-6955.
9. Gonzalez, J. M., and B. C. Carlton. 1984. A large transmissible plasmid is required for crystal toxin production in Bacillus thuringiensis variety israelensis. Plasmid 11:28-38.
10. Hofte, H., and H. R. Whiteley. 1989. Insecticidal proteins of Bacillus thuringiensis. Microbiol. Rev. 53:242-255.
11. Jensen, G. B., A. Wilcks, S. S. Petersen, J. Damgaard, J. A. Baum, and L. Andrup. 1995. The genetic basis of the aggregation system in Bacillus thuringiensis subsp. israelensis is located on the large conjugative plasmid pXO16. J. Bacteriol. 177:2914-2917.
12. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
13. Lecadet, M.-M., J. Chaufaux, J. Didier, and D. Lereclus. 1992. Construction of novel Bacillus thuringiensis strains with different insecticidal specificities by transduction and transformation. Appl. Environ. Microbiol. 58:840-849.
14. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
15. Minnich, S. A., and A. I. Aronson. 1984. Regulation of protoxin synthesis in Bacillus thuringiensis. J. Bacteriol. 158:447-454.
16. Murty, M. G., G. Srinivas, R. S. Bora, and V. Sekar. 1994. A simple method for separation of the protein crystals from Bacillus thuringiensis using carboxymethyl cellulose column chromatography. J. Microbiol. Methods 19: 103-110.
17. Ohba, M., and K. Aizawa. 1986. Crystals of Bacillus thuringiensis subsp. yunnanensis are produced only in asporogenous cells. J. Invertebr. Pathol. 48:254-256.
18. Reddy, A., L. Battisti, and C. B. Thorne. 1987. Identification of self-transmissible plasmids in four Bacillus thuringiensis subspecies. J. Bacteriol. 169: 5263-5270.
19. Ribier, J., and M.-M. Lecadet. 1973. Etude ultrastructure et cinetique de la sporulation de Bacillus thuringiensis var. berliner 1715. Remarques sur la formation de inclusion parasporale. Ann. Inst. Pasteur. 124A:311-314.
20. Ribier, J., and M.-M. Lecadet. 1981. Bacillus thuringiensis var. berliner 1715. Isolement et characterisation de mutants de sporulation. C. R. Acad. Sci. Ser. III.
21. Rubikas, J., D. Androsiuniene, G. Chestukina, T. Smirnova, O. Kapitonova, and V. Stepanov. 1987. Crystal protein formed by Bacillus subtilis cells. J. Bacteriol. 169:5258-5262.
22. Ruhfel, R. E., N. J. Robillard, and C. B. Thorne. 1984. Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis. J. Bacteriol. 157:708-711.
23. Santo, L. Y., and R. H. Doi. 1973. Crystal formation by a ribonucleic acid polymerase mutant of Bacillus subtilis. J. Bacteriol. 116:479-482.
24. Schaeffer, P., J. Millet, and J. Aubert. 1965. Catabolite repression of bacterial sporulation. Proc. Natl. Acad. Sci. USA 54:704-711.
25. Sekar, V., and B. C. Carlton. 1985. Molecular cloning of the delta-endotoxin gene of Bacillus thuringiensis var. israelensis. Gene 33:151-158.
26. Sekar, V. 1987. Location of the crystal toxin gene of Bacillus thuringiensis var. aizawai. Curr. Microbiol. 14:2893-2897.
27. Sekar, V. 1988. The insecticidal crystal protein gene is expressed in vegetative cells of Bacillus thuringiensis var. tenebrionis. Curr. Microbiol. 17:347-349.
28. Shivakumar, A. G., R. I. Vanags, D. R. Wilcox, L. K. Katz, P. S. Vary, and J. Lawrence. 1989. Gene dosage effect on the expression of delta-endotoxin genes of Bacillus thuringiensis subsp. kurstaki in Bacillus subtilis and Bacillus megaterium. Gene 79:21-31.
29. Spizizen, J. 1958. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonuclease. Proc. Natl. Acad. Sci. USA 44:1072-1078.
30. Srinivas, G., M. G. Murty, and V. Sekar. 1995. Variation in the crystal morphology of Bacillus thuringiensis ssp. yunnanensis induced by medium composition. Microbios 81:147-154.
31. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.