Direct detection of hepatitis B virus gene integrated in the Alexander cell using fluorescence in situ polymerase chain reaction

Cancer Letters

Volumn 116, Issue 2, 1997, Pages 259-264

  Matsui, Hirofumi ^a   Shiba, Reiko ^a   Matsuzaki, Yasushi ^a   Asaoka, Hitoshi ^a   Hosoi, Shigeru ^b   Doi, Mikio ^c   Ohno, Tadao ^d   Tanaka, Naomi ^a   Muto, Hiroshi ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b CENTRAL RESEARCH LABORATORY   (Japan)

^c TSUKUBA MEDICAL CENTER HOSPITAL   (Japan)

^d INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

Author keywords

Alexander cell; DNA integration; Fluorescence; Hepatitis B virus; Hepatocellular carcinoma; In situ PCR

Indexed keywords

VIRUS DNA;

ARTICLE; DNA INTEGRATION; FLUORESCENCE; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIRUS GENE;

CARCINOMA, HEPATOCELLULAR; DNA, VIRAL; FLUORESCENCE; HEPATITIS B VIRUS; HUMANS; LIVER NEOPLASMS; POLYMERASE CHAIN REACTION; TUMOR CELLS, CULTURED; VIRUS INTEGRATION;

DNA VIRUSES; HEPATITIS B VIRUS;

EID: 0030788618     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3835(97)00199-7     Document Type: Article

References (11)

1. Resnick R.H., Koff R. Hepatitis C-related hepatocellular carcinoma: prevalence and significance. Arch. Int. Med. 153:1993;1672-1677.
2. Tanaka K., Hirohata T., Koga S., Sugimachi K., Kanematsu T., Ohryohji F., Nawata H., Ishibashi H., Maeda Y., Kiyakawa H., Tokunaga K., Irita Y., Takeshita S., Arase Y., Nishino N. Hepatitis C and hepatitis B in the etiology of hepatocellular carcinoma in the Japanese population. Cancer Res. 51:1991;2842-2847.
3. Chiba T., Matsuzaki Y., Abei M., Shoda J., Tanaka N., Ohsuga T., Aikawa T. The role of previous hepatitis B virus infection and heavy smoking in hepatitis C virus-related hepatocellular carcinoma. Am. J. Gastroenterol. 91:1996;1195-1203.
4. Brechot C., Pourcel C., Louise A., Rain B., Tiollais P. Presence of integrated Hepatitis B virus DNA sequences in cellular DNA of human hepatocellular carcinoma. Nature. 286:1980;533-535.
5. Edman J.C., Gray P., Valenzuela P., Rall L.B., Rutter W.J. Integration of hepatitis B virus sequences and their expression in a human hepatoma cell. Nature. 286:1980;535-537.
6. Seufer M., Hohne M., Schaefer S., Gerlich W.H. In vitro tumorigenicity of hepatitis B virus DNA and HBx protein. J. Hepatol. 13:1991;61-65.
7. Kim C.M., Koike K., Saitoh I., Miyamura T., Jay G. HBx gene of hepatitis B virus induced liver cancer in transgenic mice. Nature. 351:1991;317-320.
8. Natoli G., Avantaggiati M.L., Balsano C., de Marzio E., Collepardo D., Elfassi E., Levrero M. Characterization of hepatitis B virus preS/S region encoded transcriptional transactivator. Virology. 187:1992;663-670.
9. Nuovo G.J., Becher J., Simsir A., Margiotta M., Khalife G., Shevchuk M. HIV-1 nucleic acids localize to the spermatogonia and their progeny. Am. J. Pathol. 144:1994;1142-1148.
10. Patterson B.K., Till M., Otto P., Goolsby C., Furtado M.R., McBride L.J., Wolinsky S.M. Detection of HIV-1 DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry. Science. 260:1993;976-979.
11. Schmidtmayerova H., Nottet H.S.L.M., Nuovo G., Raabe T., Flanagan C.R., Dubrovsky L., Gendelman H.E., Cerami A., Bukrinsky M., Sherry B. Human immunodeficiency virus type 1 infection alters chemokine beta peptide expression in human monocytes - implications for recruitment of leukocytes into brain and lymph nodes. Proc. Natl. Acad. Sci. USA. 93:1996;700-704.