Involvement of the gonococcal MtrE protein in the resistance of Neisseria gonorrhoeae to toxic hydrophobic agents

Microbiology

Volumn 143, Issue 7, 1997, Pages 2127-2133

  Delahay, R M ^a   Robertson, B D ^a   Balthazar, J T ^b   Shafer, W M ^b,c   Ison, C A ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b ATLANTA VA MEDICAL CENTER   (United States)

^c EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Antimicrobial resistance; Efflux; Hydrophobic agents; MtrE; Neisseria gonorrhoeae

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL PROTEIN; CARRIER PROTEIN; OUTER MEMBRANE PROTEIN;

ALPHA HELIX; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM MUTANT; CONTROLLED STUDY; DRUG TRANSPORT; HYDROPHOBICITY; MULTIDRUG RESISTANCE; NEISSERIA GONORRHOEAE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PSEUDOMONAS AERUGINOSA; TRANSPOSON;

BACTERIA (MICROORGANISMS); NEISSERIA GONORRHOEAE; NEISSERIA MENINGITIDIS; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0030788246     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-7-2127     Document Type: Article

References (33)

1. Abadi, F. J. R., Carter, P. E., Cash, P. & Pennington, T. H. (1996). Rifampin resistance in Neisseria meningitidis due to alterations in membrane permeability. Antimicrob Agents Chemother 40, 646-651.
2. Brendel, V., Bucher, P., Nourbakhsh, I. R., Blaisdell, E. & Karlin, S. (1992). Methods and algorithms for statistical analysis of protein sequences. Proc Natl Acad Sci USA 89, 2002-2006.
3. Burns, J. L., Wadsworth, C. D., Barry, J. J. & Goodall, C. P. (1996). Nucleotide sequence analysis of a gene from Burkholderia (Pseudomonas) cepacia encoding an outer membrane lipoprotein involved in multiple antibiotic resistance. Antimicrob Agents Chemother 40, 307-313.
4. Chou, P. Y. & Fasman, G. D. (1978). Empirical prediction of protein conformation. Annu Rev Biochem 47, 251-276.
5. Correia, F. F., Inouye, S. & Inouye, M. (1988). A family of small repeated elements with some transposon-like properties in the genome of Neisseria gonorrhoeas. J Biol Chem 263, 12194-12198.
6. Devereux, J., Haeberli, P. & Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12, 387-395.
7. 2+-binding protein that is exported without N-terminal cleavage and is homologous to haemolysin and related proteins. EMBO J 9, 349-354.
8. Fath, M. J. & Kolter, R. (1993). ABC transporters: bacterial exporters. Microbiol Rev 57, 995-1017.
9. Fralick, J. A. & Burns-Keliher, L. L. (1994). Additive effect of tolC and rfa mutations on the hydrophobic barrier of the outer membrane of Escherichia coli K-12. J Bacteriol 176, 6404-6406.
10. Garnier, J., Osguthorpe, D. J. & Robson, B. (1978). Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol 120, 97-120.
11. Goodman, S. D. & Scocca, J. J. (1988). Identification and arrangement of the DNA sequence recognised in specific transformation of Neisseria gonorrhoeas. Proc Natl Acad Sci USA 85, 6982-6986.
12. Guymon, L. F., Walstad, D. L. & Sparling, P. F. (1978). Cell envelope alterations in antibiotic-sensitive and -resistant strains of Neisseria gonorrhoeae. J Bacteriol 136, 391-401.
13. Hagman, K. E. & Shafer, W. M. (1995). Transcriptional control of the mtr efflux system of Neisseria gonorrhoeae. J Bacteriol 177, 4162-4165.
14. Hagman, K. E., Pan, W., Spratt, B. G., Balthazar, J. T., Judd, R. C. & Shafer, W. S. (1995). Resistance of Neisseria gonorrhoeae to antimicrobial hydrophobic agents is modulated by the mtrRCDE efflux system. Microbiology 141, 611-622.
15. Hayashi, S. & Wu, H. C. (1990). Lipoproteins in bacteria. J Bioenerg Biomembr 22, 451-471.
16. Kyte, J. & Doolittle, R. F. (1982). A simple method for displaying the hydropathic character of a protein. J Mol Biol 157, 105-132.
17. Lucas, C. E., Hagman, K. E., Levin, J. C., Stein, D. C. & Shafer, W. M. (1995). Importance of lipooligosaccharide structure in determining gonococcal resistance to hydrophobic antimicrobial agents resulting from the mtr efflux system. Mol Microbiol 16, 1001-1009.
18. Ma, D., Cook, D. N., Alberti, M., Pon, N. G., Nikaido, H. & Hearst, J. E. (1993). Molecular cloning and characterisation of acrA and acrE genes of Escherichia coli. J Bacteriol 175, 6299-6313.
19. Ménard, R., Sansonetti, P. J. & Parsot, C. (1993). Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J Bacteriol 175, 5899-5906.
20. Morona, R., Manning, P. A. & Reeves, P. (1983). Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli. J Bacteriol 153, 693-699.
21. Morse, S. A., Lysko, P. G., McFarland, L., Knapp, J. S., Sandstrom, E., Critchlow, C. & Holmes, K. K. (1982). Gonococcal strains from homosexual men have outer membranes with reduced permeability to hydrophobic molecules. Infect Immun 37, 432-438.
22. Nikaido, H. (1994). Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 264, 382-387.
23. Nikaido, H. & Saier, M. H., Jr (1992). Transport proteins in bacteria: common themes in their design. Science 258, 936-942.
24. Pan, W. & Spratt, B. G. (1994). Regulation of the permeability of the gonococcal cell envelope by the mtr system. Mol Microbiol 11, 769-775.
25. Poole, K., Krebes, K., McNally, C. & Neshat, S. (1993). Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon. J Bacteriol 175, 7363-7372.
26. Rost, B. & Sander, C. (1993). Prediction of protein secondary structure at better than 70% accuracy. J Mol Biol 232, 584-599.
27. Saier, M. H., Jr, Tam, R., Reizer, A. & Reizer, J. (1994). Two novel families of bacterial membrane proteins concerned with nodulation, cell division and transport. Mol Microbiol 11, 841-847.
28. Shafer, W. M., Guymon, L. F., Lind, I. & Sparling, P. F. (1984). Identification of an envelope mutation (env-10) resulting in increased antibiotic susceptibility and pyocin resistance in a clinical isolate of Neisseria gonorrhoeae. Antimicrob Agents Chemother 25, 767-769.
29. Shafer, W. M., Balthazar, J. T., Hagman, K. E. & Morse, S. A. (1995). Missense mutations that alter the DNA-binding domain of the MtrR protein occur frequently in rectal isolates of Neisseria gonorrhoeae that are resistant to faecal lipids. Microbiology 141, 907-911.
30. Sparling, P. F., Sarubbi, F. A., Jr & Blackman, E. (1975). Inheritance of low-level resistance to penicillin, tetracycline, and chloramphenicol in Neisseria gonorrhoeae. J Bacteriol 124, 740-749.
31. Stern, A., Nickel, P., Meyer, T. F. & So, M. (1984). Opacity determinants of Neisseria gonorrhoeae: gene expression and chromosomal linkage to the gonococcal pilus gene. Cell 37, 447-456.
32. Surin, B. P., Watson, J. M., Hamilton, W. D., Economou, A. & Downie, J. A. (1990). Molecular characterisation of the nodulation gene, nodT, from two biovars of Rhizobium leguminosarum. Mol Microbiol 4, 245-252.
33. Wang, R. C., Seror, S. J., Blight, M., Pratt, J. M., Broome-Smith, J. K. & Holland, I. B. (1991). Analysis of the membrane organization of an Escherichia coli protein translocator, HlyB, a member of a large family of prokaryote and eukaryote surface transport proteins. J Mol Biol 217, 441-454.