Electrophoretic separation and identification of phenoloxidases in hemolymph and midgut of adult Anopheles stephensi mosquitoes

Journal of Parasitology

Volumn 83, Issue 4, 1997, Pages 686-691

  Sidjanski, Sacha ^a   Mathews, Grace V ^a   Vanderberg, Jerome P ^a  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ISOENZYME; MONOPHENOL MONOOXYGENASE;

ADOLESCENT; ANIMAL TISSUE; ANOPHELES STEPHENSI; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME SUBSTRATE; FEMALE; GEL ELECTROPHORESIS; HEMOLYMPH; MIDGUT; NONHUMAN;

ANIMALS; ANOPHELES; CENTRIFUGATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; HEMOLYMPH; INSECT VECTORS; ISOENZYMES; MOLECULAR WEIGHT; MONOPHENOL MONOOXYGENASE;

ANIMALIA; ANOPHELES MOSQUITO; ANOPHELES STEPHENSI;

EID: 0030787829     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.2307/3284247     Document Type: Article

References (27)

1. AHMAD, S., C. J. LEAKE, AND A. J. KETTERMAN. 1995. Qualitative differences occur in phenoloxidase activity in Anopheles mosquitoes refractory to Plasmodium. Biochemical Society Transactions 23: 110S.
2. ASHIDA, M., K. KINOSHITA, AND P. T. BREY. 1990. Studies on prophenoloxidase activation in the mosquito Aedes aegypti. European Journal of Biochemistry 188: 507-515.
3. BREY, P. T.. A. AHMED. W.-J. LEE, M. ASHIDA, AND M. J. LEHANE. 1995. Tyrosinase-type prophenoloxidase distribution in the alimentary canal of strains of Anopheles gambiae refractory and susceptible to Plasmodium infection. Experimental Parasitology 80: 654-664.
4. BRUNET, P. C. J. 1980. The metabolism of the aromatic amino acids concerned in the cross-linking of insect cuticle. Insect Biochemistry 10: 467-500.
5. CHRISTENSEN, B. M., B. M. HUFF, G. S. MIRANPURI, K. L. HARRIS, AND L. A. CHRISTENSEN. 1989. Hemocyte population changes during the immune response of Aedes aegypti to inoculated microfilariae of Dirofilaria immitis. Journal of Parasitology 75: 119-123.
6. _, AND J. W. TRACY. 1989. Arthropod-transmitted parasites: Mechanisms of immune interaction. American Zoologist 29: 387-398.
7. COLLINS, F. H., R. K. SAKAI, K. D. VERNICK, S. PASKEWITZ, D. C. SEELEY, L. H MILLER, W. E. COLLINS, C. C. CAMPBELL, AND R. W. GWADZ. 1986. Genetic selection of a Plasmodium-refractory strain of the malaria vector Anopheles gambiae. Science 234: 607-610.
8. KAHN, V. 1985. Tropolone - A compound that can aid in differentiating between tyrosinase and peroxidase. Phytochemistry 24: 915-920.
9. _, AND A. ANDRAWIS. 1985. Inhibition of mushroom tyrosinase by tropolone. Phytochemistry 24: 905-908.
10. LI, J., J. W. TRACY, AND B. M. CHRISTENSEN. 1992. Relationship of hemolymph phenol oxidase and mosquito age in Aedes aegypti. Journal of Invertebrate Pathology 60: 188-191.
11. MACK, S. R., D. A. FOLEY, AND J. P. VANDERBERG. 1979. Hemolymph volume of non-infected and Plasmodium berghei-infected Anopheles stephensi mosquitoes. Journal of Invertebrate Pathology 34: 105-109.
12. _, S. SAMUELS, AND J. P VANDERBERG. 1979a. Hemolymph of Anophe0les stephensi from non-infected and Plasmodium bergheiinfected mosquitoes. 2. Free amino acids. Journal of Parasitology 65: 130-136.
13. _, _, AND _. 1979b. Hemolymph of Anopheles stephensi from noninfected and Plasmodium berghei-infected mosquitoes. 3. Carbohydrates. Journal of Parasitology 65: 217-221.
14. _, AND J. P. VANDERBERG. 1978. Hemolymph of Anopheles stephensi from noninfected and Plasmodium berghei-infected mosquitoes. 1. Collection procedure and physical characteristics. Journal of Parasitology 64: 918-923.
15. NELLAIAPPAN, K., AND M. J. BANU. 1991. Demonstration of monophenoloxidase activity of tyrosinase after electrophoresis. Biotechnic and Histochemistry 66: 125-130.
16. _, AND K. VALIVITTAN. 1993. Differentiation of tyrosine hydroxylase and phenol oxidase after electrophoresis. Biotechnic and Histochemistry 68: 281-283.
17. _, AND A. VINAYAGAM. 1986. A rapid method for detection of tyrosinase activity in electrophoresis. Stain Technology 61: 269-283.
18. OKUN, M. R., L. M. EDELSTEIN, N. OR, G. HAMADA, B. DONNELLAN, AND W. F. LEVER. 1970. Histochemical differentiation of peroxidase-mediated from tyrosine-mediated melanin formation in mammalian tissues. The biologic significance of peroxidase-mediated oxidation of tyrosine to melanin. Histochemie 23: 295-309.
19. PASKEWITZ, S. M., M. R. BROWN, F. H. COLLINS, AND A. O. LEA. 1989. Ultrastructural localization of phenoloxidase in the midgut of refractory Anopheles gambiae and association of the enzyme with encapsulated Plasmodium cynomolgi. Journal of Parasitology 75: 594-600.
20. _, _, A. O. LEA, AND F. H. COLLINS. 1988. Ultrastructure of the encapsulation of Plasmodium cynomolgi (B strain) on the midgut of a refractory strain of Anopheles gambiae. Journal of Parasitology 74: 432-439.
21. PHASTSYSTEM OWNERS MANUAL. 1986. Laboratory Separation Division, Pharmacia-LKB, Uppsala, Sweden, 45 p.
22. RIBEIRO, J. C., AND R. H. NUSSENZVEIG. 1993. The salivary catechol oxidase/peroxidase activities of the mosquito Anopheles albimanus. Journal of Experimental Biology 179: 273-287.
23. _, _, AND G. TORTORELLA. 1994. Salivary vasodilators of Aedes triseriatus and Anopheles gambiae. Journal of Medical Entomology 31: 747-753.
24. ROTHE, G. M., AND W. D. MAURER. 1986. One-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. In Gel electrophoresis of proteins, M. J. Dunn (ed.). IOP Publishing Ltd., Bristol, U.K., p. 37-140.
25. SUGUMARAN, M., L. GIGLIO, H. KUNDZICZ, S. SAUL, AND V. SEMENSI. 1992. Studies on the enzymes involved in puparial cuticle sclerotization in Drosophila melanogaster. Archives of Insect Biochemistry and Physiology 19: 271-283.
26. YAMAZAKI, H. I. 1969. The cuticular phenoloxidase in Drosophila virilis. Journal of Insect Physiology 15: 2203-2211.
27. _. 1972. Cuticular phenoloxidase from the silkworm, Bombyx mori: Properties, solubilization, and purification. Insect Biochemistry 2: 431-444.