Identification of new autolytic sites of recombinant truncated mature human fibroblast stromelysin by mass spectrometry

Journal of Peptide Research

Volumn 49, Issue 6, 1997, Pages 612-619

  Qoronfleh, M Walid ^a   Chowdhury, Swapan K ^b   Eshraghi, Jamshid ^b   Ho, Thau ^b   Brake, Patricia G ^b   Banks, Tracey ^b   Huang, James ^b   Pulvino, Tricia ^b   Jones, Barry N ^b  

^a SAIC FREDERICK INC   (United States)

^b Sanofi Winthrop Inc   (United States)

Author keywords

Autodegradation; Mass spectrometry; Metalloproteinase; MMP 3; Mutein (mutant protein); Recombinant stromelysin

Indexed keywords

ARGININE; LEUCINE; MUTANT PROTEIN; RECOMBINANT ENZYME; STROMELYSIN; STROMELYSIN INHIBITOR;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CARTILAGE DEGENERATION; ENZYME ACTIVE SITE; ENZYME PURIFICATION; FIBROBLAST; HUMAN; HUMAN CELL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; OSTEOARTHRITIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; RHEUMATOID ARTHRITIS;

EID: 0030785645     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-3011.1997.tb01170.x     Document Type: Article

References (25)

1. Galloway, W.A., Murphy, G., Sandy, J.D., Gavrilovic, J., Cawston, T.E. & Reynolds, J.J. (1983) Biochem. J. 209, 741-752
2. Chin, J.R., Murphy, G. & Werb, Z. (1985) J. Biol. Chem. 260, 12367-12376
3. Okada, Y., Nagase, H & Harris, E.D. (1986) J. Biol. Chem. 261, 14245-14255
4. Wilhelm, S.M., Collier, I.E., Kronberger, A., Eisen, A., Marmer, B., Grant, B., Bauer, E. & Goldberg, G. (1987) Proc. Natl. Acad. Sci. USA 84, 6725-6729
5. Docherty, A.J.P., O'Connell, J., Crabbe, T., Angal, S. & Murphy, G. (1992) TIBTECH 10, 200-207
6. Van Wart, H.E. & Birkedal-Hansen, H. (1990) Proc. Natl. Acad. Sci. USA 87, 5578-5582
7. Walakovits, L.A., Moore, V.L., Bhardwaj, N., Gallick, G.S. & Lark, M.W. (1992) Arthritis and Rheumatism 35, 35-42
8. Wahl, R., Dunlap, R. & Morgan, B. (1989) Annu. Rep. Med. Chem. 25, 177-184
9. Henderson, B., Docherty, A.J.P. & Beeley, N.R.A. (1990) Drugs of the Future 15, 495-508
10. Okada, Y., Harris, E.D. & Nagase, H. (1988) Biochem. J. 254, 731-741
11. Nagase, H., Enghild, J.J., Suzuki, K. & Salvesen, G. (1990) Biochemistry 29, 5783-5789
12. Ye, Q.Z., Johnson, L.L., Hupe, D.J. & Baragi, V. (1992) Biochemistry 31, 11231-11235
13. Marcy, A.I., Eiberger, L.L., Harrison, R., Chan, H.K., Hutchinson, N.I., Hagmann, W.K., Cameron, P.M., Boulton, D.S. & Hermes, J.D. (1991) Biochemistry 30, 6476 -6483
14. Qoronfleh, M.W., Ho, T., Banks, T., Pulvino, T., Brake, P., Ciccarelli, R., Koehn, J., Huang, J. & Wahl, R. (1996) Protein Peptide Lett. 3, 233-240
15. Fenn, J.B., Mann, M., Meng, C.K., Wong, S.F. & Whitehouse, C.M. (1990) Mass Spectrom. Rev. 9, 37-70
16. Smith, R.D., Loo, J.A., Ogorzalek-Loo, R.R., Busman, M. & Udseth, H.R. (1991) Mass Spectrom. Rev. 10, 359-452
17. Villafranca, J.J., ed. (1991) Techniques in Protein Chemistry, II, Academic Press, New York
18. Hunt, D.F., Michel, H., Dickinson, T.A., Shabanowitz, J., Cox, A.L., Sakaguchi, K., Appella, E., Grey, H.M. & Sette, A. (1992) Science 256, 1817-1820
19. Carr, S.A., Hemling, M.E., Bean, M.F. & Roberts, G.D. (1991) Anal. Chem. 63, 2802-2824
20. Huang, E.C., Wachs, T., Conboy, J.J. & Henion, J.D. (1990) Anal. Chem. 63, 713A-725A
21. Eshraghi, J. & Chowdhury, S.K. (1993) Anal. Chem. 65, 3528-3533
22. Ho, T.F., Qoronfleh, M.W., Wahl, R.C., Pulvino, T.A., Vavra, K.J., Banks, T.M., Brake, P.G. & Ciccarelli, R.B. (1994) Gene 146, 297-301
23. Moore, W.M. & Spilburg, C.A. (1986) Biochemistry 25, 5189-5195
24. Chait, B.T. & Kent, S.B.H. (1992) Science 257, 1885-1894
25. Rosenfeld, S.A., Ross, O.H., Corman, J.I., Pratta, M.A., Blessington, D.L., Feeser, W.S. & Freimark, B.D. (1994) Gene 139, 281-286