Delayed triphenyltetrazolium chloride staining remains useful for evaluating cerebral infarct volume in a rat stroke model

Journal of Cerebral Blood Flow and Metabolism

Volumn 17, Issue 10, 1997, Pages 1132-1135

  Li, Fuhai ^a   Irie, Katsumi ^a   Anwer, M Sawkat ^c   Fisher, Marc ^a,b  

^a Memorial Health Care ^*  (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Experimental stroke; Infarction; Succinate dehydrogenase; TTC staining

Indexed keywords

SUCCINATE DEHYDROGENASE; TRIPHENYLTETRAZOLIUM;

ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN INFARCTION; CONTROLLED STUDY; DISEASE SEVERITY; ENZYME ACTIVITY; EXPERIMENTAL MODEL; HISTOPATHOLOGY; MALE; MIDDLE CEREBRAL ARTERY OCCLUSION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; RAT; STROKE;

EID: 0030783187     PISSN: 0271678X     EISSN: None     Source Type: Journal    
DOI: 10.1097/00004647-199710000-00016     Document Type: Article

References (23)

1. Bederson JB, Pitts LH, Germano SM, Nishimura MC, Davis RL, Bartkowski HM (1986) Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats. Stroke 17:1304-1308
2. Bose B, Osterholm JL, Berry R (1984) A reproducible experimental model of focal cerebral ischemia in the cat. Brain Res 311:385-391
3. Cole DJ, Drummond JC, Ghazal EA, Shapiro HM (1990) A reversible component of cerebral injury as identified by the histochemical stain 2,3,5-triphenyltetrazolium chloride (TTC). Acta Neuropathol 80:152-155
4. Dettmers C, Hartmann A, Rommel T, Kramers S, Pappata S, Young A, Hartmann S, Zierz S, Mackenzie ET, Baron J-C (1994) Triphenyltetrazolium chloride (TTC) compared to mitochondrial enzymes 6 hours after MCA-occlusion in primates. Neurol Res 16:205-208
5. Hasegawa H, Latour LL, Formato JE, Sotak CH, Fisher M (1995) Spreading waves of a reduced diffusion coefficient of water in normal and ischemic rat brain. J Cereb Blood Flow Metab 15:179-187
6. Hatfield RH, Mendelow AD, Perry RH, Alvarezs LM, Modha P (1991) Triphenyltetrazolium chloride (TTC) as a marker for ischemic changes in rat brain following permanent middle cerebral artery occlusion. Neuropathol Appl Neurobiol 17:61-67
7. Hochstadt J, Quinlan DC, Rader RL, Li C-C, Dowd D (1974) Use of isolated membrane vesicles in transport studies. In: Methods in Membrane Biology, vol 5 (Korn E, ed). New York, Plenum Press, pp 117-162
8. Isayama K, Pitts LH, Nishimura MC (1991) Evaluation of 2,3,5-triphenyltetrazolium chloride staining to delineate rat brain infarcts. Stroke 22:1394-1398
9. Koizumi J, Yoshida Y, Nakazawa T, Ooneda G (1986) Experimental studies of ischemic brain edema. I: a new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke 8:1-8
10. Kuge Y, Minematsu K, Yamaguchi T, Miyake Y (1995) Nylon monofilament for intraluminal middle cerebral artery occlusion in rats. Stroke 26:1655-1658
11. Lin T-N, He YY, Wu G, Khan M, Hsu CY (1993) Effect of brain edema on infarct volume in a focal cerebral ischemia model in rats. Stroke 24:117-121
12. Lippold HJ (1982) Quantitative succinic dehydrogenases histochemistry: a comparison of different tetrazolium salts. Histochemistry 76:381-405
13. Liszczak TM, Hedley-Whyte ET, Adams JF, Han DH, Kolluri VS, Vacanti FX, Heros RC, Zervas NT (1984) Limitation of tetrazolium salts in delineating infarcted brain. Acta Neuropathol (Berl) 65:150-157
14. Lowry DH, Rosenberg NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265-275
15. Lundy EF, Solik BS, Frank RS, Lacy PS, Combs DJ, Zelenock GB, D'Alecy LG (1986) Morphometric evaluation of brain infarcts in rats and gerbils. J Pharmacol Methods 16:201-214
16. Memezawa H, Minamisawa H, Smith M-L, Siesjo BK (1992) Ischemic penumbra in a model of reversible middle cerebral artery occlusion in the rat. Exp Brain Res 89:67-78
17. Park CK, Mendelow AD, Graham DI, Mcculloch J, Teasdale GM (1988) Correlation of triphenyltetrazolium chloride perfusion staining with conventional neurohistology in the detection of early brain ischemia. Neuropathol Appl Neurobiol 14:289-298
18. Reith W, Hasegawa Y, Latour LL, Dardzinski BJ, Sotak CH, Fisher M (1995) Multislice diffusion mapping for 3-D evolution of cerebral ischemia in a rat stroke model. Neurology 45:172-177
19. Schabitz W-R, Weber J, Takano K, Sandage BW, Loche KW, Fisher M (1996) The effects of prolonged treatment with citicoline in temporary experimental focal ischemia. J Neurol Sci 138:21-25
20. Swanson RA, Morton MT, Tsao-Wu G, Savalos RA, Davidson C, Sharp FR (1990) A semiautomated method for measuring brain infarct volume. J Cereb Blood Flow Metab 10:290-293
21. Takano K, Latour LL, Formato JE, Carano RAD, Helmer KG, Hasegawa Y, Sotak CH, Fisher M (1996) The role of spreading depression in focal ischemia evaluated by diffusion mapping. Ann Neurol 39:308-318
22. Taylor R, Luk YO, Chen ST, Balentine JD, Hogan EL, Hsu CY (1987) Quantitation of infarct area by triphenyl tetrazolium chloride (TTC) in a rat stroke model. Neurology 37(Suppl 1):82
23. Zea Longa E, Weinstein PR, Carlson S, Cummins R (1989) Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 20:84-91