Flow cytometric analysis of chlorhexidine action

FEMS Microbiology Letters

Volumn 154, Issue 2, 1997, Pages 283-288

  Sheppard, Fiona C ^a   Mason, David J ^b   Bloomfield, Sally F ^a   Gant, Vanya A ^b  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

Chlorhexidine; Escherichia coli; Flow cytometry; Membrane integrity; Membrane potential

Indexed keywords

CHLORHEXIDINE;

ARTICLE; BACTERICIDAL ACTIVITY; BACTERIUM; COLONY FORMING UNIT; CONTROLLED STUDY; DRUG UPTAKE; ESCHERICHIA COLI; FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL;

ANTI-INFECTIVE AGENTS, LOCAL; CELL MEMBRANE; CHLORHEXIDINE; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; LIGHT; SCATTERING, RADIATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030782988     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00343-1     Document Type: Article

References (18)

1. Rye, R.M. and Wiseman, D. (1964) Release of phosphorus-32-containing compounds from Micrococcus lysodeikticus treated with chlorhexidine. J. Pharm. Pharmacol. 20, 14-17.
2. Hugo, W.B. and Longworth, A.R. (1964) Some aspects of the mode of action of chlorhexidine. J. Pharm. Pharmacol. 16, 665-662.
3. Hugo, W.B. and Longworth, A.R. (1965) Cytological aspects of the mode of action of chlorhexidine diacetate. J. Pharm. Pharmacol. 17, 28-32.
4. Chawner, J.A. and Gilbert, P. (1989) Interaction of the bisbiguanides chlorhexidine and alexidine with phospholipid vesicles: evidence for separate modes of action. J. Appl. Bacteriol. 66, 253-258.
5. Hugo, W.B. and Longworth, A.R. (1966) The effect of chlorhexidine on the electrophoretic mobility, cytoplasmic constituents, dehydrogenase activity and cell walls of Escherichia coli and Staphylococcus aureus. J. Pharm. Pharmacol. 18, 569-578.
6. Harold, F.M., Barada, J.R., Baron, C. and Abrams, A. (1969) Dio 9 and chlorhexidine inhibition of membrane bound ATPase and of cation transport in Streptococcus faecalis. Biochim. Biophys. Acta 183, 129-136.
7. Chopra, I., Johnson, S.C. and Bennett, P.M. (1987) Inhibition of Providencia stuartii cell envelope enzymes by chlorhexidine. J. Antimicrob. Chemother. 19, 743-751.
8. Kuyyakanond, T. and Quesnel, L.B. (1992) The mechanism of action of chlorhexidine. FEMS Microbiol. Lett. 100, 211-216.
9. 2+ sensitive potassium channels described by oxonol dye fluorescence measurements. J. Cell Physiol. 125, 72-81.
10. Mason, D.J., Power, E.G.M., Talsania, H., Phillips, I. and Gant, V.A. (1995) Antibacterial action of ciprofloxacin. Antimicrob. Agents Chemother. 39, 2752-2758.
11. (106-137). Antimicrob. Agents Chemother. 41, 624-629.
12. Gant, V.A., Warnes, G., Phillips, I. and Savidge, G.F. (1993) The application of flow cytometry to the study of bacterial responses to antibiotics. J. Med. Microbiol. 39, 147-154.
13. Sheppard, F.C. (1996) The application of rapid methods to the preservative efficacy test. PhD Thesis, University of London.
14. Miles, A.A., Misra, S.S. and Irwin, J.O. (1938) The estimation of the bactericidal power of the blood. J. Hygiene 38, 732-749.
15. Ismaeel, N., Furr, J.R. and Russell, A.D. (1986) Reversal of the surface effects of chlorhexidine on cells of Providencia stuartii. J. Appl. Bacteriol. 61, 373-381.
16. Chapman, J.S., Diehl, M.A. and Lyman, R.C. (1993) Biocide susceptibility and intracellular glutathione in Escherichia coli. J. Indust. Microbiol. 12, 403-407.
17. Gilbert, P., Collier, P.J. and Brown, M.R.W. (1990) Influence of growth rate on susceptibility to antimicrobial agents: biofilms, cell cycle, dormancy and stringent response. Antimicrob. Agents Chemother. 34, 1865-1868.
18. Matin, A. (1992) Physiology, molecular biology and applications of the bacterial starvation response. J. Appl. Bacteriol. Symp. Suppl. 73, 49s-57s.