Improvement of herpesvirus saimiri T cell immortalization procedure to generate multiple CD4 + T-cell clones from peripheral blood lymphocytes of AIDS patients

Journal of Immunological Methods

Volumn 206, Issue 1-2, 1997, Pages 21-23

  Saha, Kunal ^a   McKinley, George ^a   Volsky, David J ^a  

^a ST LUKE S ROOSEVELT HOSPITAL CENTER   (United States)

Author keywords

AIDS; CD4+ cells; Herpesvirus saimiri; Immortalization

Indexed keywords

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ARTICLE; CELL IMMORTALIZATION; HERPES VIRUS SAIMIRI; HUMAN; HUMAN CELL; LYMPHOCYTE SUBPOPULATION; PRIORITY JOURNAL; T LYMPHOCYTE;

ACQUIRED IMMUNODEFICIENCY SYNDROME; CD4 LYMPHOCYTE COUNT; CD4-POSITIVE T-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELL TRANSFORMATION, VIRAL; CLONE CELLS; HERPESVIRUS 2, SAIMIRIINE; HUMANS; LYMPHOCYTE ACTIVATION;

HERPES; HERPESVIRIDAE; HUMAN IMMUNODEFICIENCY VIRUS 1; SAIMIRI; SAIMIRIINE HERPESVIRUS 2;

EID: 0030782956     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00080-X     Document Type: Article

References (11)

1. Biesinger, B., Muller-Fleckenstein, I., Simmer, B., Lang, G., Wittmann, S., Platzer, E., Desrosiers, R.C., Fleckenstein, B., 1992. Stable growth transformation of human T lymphocytes by Herpesvirus saimiri. Proc. Natl. Acad. Sci. USA 89, 3116.
2. Broker, B.M., Tsygankov, A.Y., Muller-Fleckenstein, I., Guse, A.H., Chitaev, N.A., Biesinger, B., Fleckenstein, B., Emmrich, F., 1993. Immortalization of human T cell clones by Herpesvirus saimiri. Signal transduction analysis reveals functional CD3, CD4, and IL-2 receptors. J. Immunol. 151, 1184.
3. Del Prete, G., Carli, M.D., D'Elios, M.M., Muller-Fleckenstein, I., Fickenscher, H., Fleckenstein, B., Almerigogna, F., Romagnani, S., 1994. Polyclonal B cell activation induced by Herpesvirus saimiri-transformed human CD4+ T cell clones: Role for membrane TNF-/TNF- receptors and CD2/CD58 interactions. J. Immunol. 147, 3389.
4. Meinl, E., Hohlfeld, R., Wekerle, H., Fleckenstein, B., 1995. Immortalization of human T cells by Herpesvirus saimiri. Immunol. Today 16, 55.
5. Mittrucker, H.W., Muller-Fleckenstein, I., Fleckenstein, B., Fleischer, B., 1993. Herpesvirus saimiri-transformed human T lymphocytes: Normal functional phenotype and preserved T-cell receptor signaling. Int. Immunol. 5, 985.
6. Moriuchi, H., Moriuchi, M., Combadiere, C., Murphy, P.M., Fauci, A.S., 1996. CD8+ T-cell-derived soluble factor(s), but not -chemokines RANTES, MIP-1, and MIP-1, suppress HIV-1 replication in monocyte/macrophage. Proc. Natl. Acad. Sci. USA 93, 15341.
7. Nick, S., Fickenscher, H., Biesinger, B., Born, G., Jahn, G., Fleckenstein, B., 1993. Herpesvirus saimiri transformed human T cell lines: A permissive system for human immunodeficiency viruses. Virology 194, 875.
8. Saha, K., Sova, P., Chao, W., Chess, L., Volsky, D.J., 1996a. Generation of CD4+ and CD8+ T-cell clones from PBLs of HIV-1 infected subjects using Herpesvirus saimiri. Nature Med. 2, 1272.
9. Saha, K., Ware, R., Yellin, M.J., Chess, L., Lowy, I., 1996b. Herpesvirus saimiri-transformed human CD4+ T cells can provide polyclonal B cell help via the CD40 ligand as well as the TNF-pathway and through release of lymphokines. J. Immunol. 157, 3876.
10. Saha, K., Caruso, M., Volsky, D.J., 1997. Human immunodeficiency virus type 1 (HIV-1) infection of Herpesvirus saimiri-immortalized human CD4-positive T lymphoblastoid cells: Evidence of enhanced HIV-1 replication and cytopathic effects caused by endogenous IFN-γ. Virology 231, 1.
11. Weber, F., Meinl, E., Drexler, K., Czionkowska, A., Huber, S., Fickenscher, H., Muller-Fleckenstein, I., Fleckenstein, B., Wekerle, H., Hohlfeld, R., 1993. Transformation of human T-cell clones by herpesvirus saimiri: Intact antigen recognition by autonomously growing myelin basic protein-specific T cells. Proc. Natl. Acad. Sci. USA 90, 11049.