Allele-specific PCR assays for the tub and cpe(fat) mutations

Mammalian Genome

Volumn 8, Issue 11, 1997, Pages 857-858

  Maddatu, Terry ^a   Naggert, Jürgen K ^a  

^a JACKSON LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYPEPTIDASE; CARBOXYPEPTIDASE H; PRIMER DNA; PROTEIN; TUB PROTEIN, MOUSE;

ALLELE; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CROSS BREEDING; FEMALE; GENETICS; MALE; METHODOLOGY; MOLECULAR GENETICS; MOUSE; MOUSE MUTANT; OBESITY; POLYMERASE CHAIN REACTION;

ALLELES; AMINO ACID SEQUENCE; ANIMALS; CARBOXYPEPTIDASE H; CARBOXYPEPTIDASES; CROSSES, GENETIC; DNA PRIMERS; FEMALE; MALE; MICE; MICE, MUTANT STRAINS; MOLECULAR SEQUENCE DATA; OBESITY; POLYMERASE CHAIN REACTION; PROTEINS;

EID: 0030781737     PISSN: 09388990     EISSN: None     Source Type: Journal    
DOI: 10.1007/s003359900594     Document Type: Article

References (8)

1. Coleman DL, Eicher EM (1990) Fat (fat) and tubby (tub), two autosomal recessive mutations causing obesity syndromes in the mouse. J Hered 81, 424-427
2. Fricker LD, Berman YL, Leiter EH, Devi LA (1996) Carboxypeptidase E activity is deficient in mice with the fat mutation. Effect on peptide processing. J Biol Chem 271, 30619-30624
3. Heckenlively JR, Chang B, Erway LC, Peng C, Hawes NL, Hageman GS, Roderick TH (1995) Mouse model for Usher syndrome: linkage mapping suggests homology to Usher type I reported at human chromosome 11p15. Proc Natl Acad Sci USA 92, 11100-11104
4. Kleyn PW, Fan W, Kovats SG, Lee JJ, Pulido JC, Wu Y, Berkenmeier LR, Misumi DJ, Holmgren L, Charlat O, Woolf EA, Tayber O, Brody T, Shu P, Hawkins F, Kennedy B, Baldini L, Ebeling C, Alperin GD, Deeds J, Lakey ND, Culpepper J, Chen H, Glüksmann-Kuis MA, Carlson GA, Duyk GM, Moore KJ (1996) Identification and characterization of the mouse obesity gene tubby: a member of a novel gene family. Cell 65, 281-290
5. Naggert JK, Fricker LD, Varlamov O, Nishina PM, Rouilla Y, Steiner DF, Carrol RJ, Paigen BJ, Leiter EH (1995) Hyperproinsulinaemia in obese fat/fat mice associated with a carboxypeptidase E mutation which reduces enzyme activity. Nature Genet 10, 135-142
6. Noben-Trauth K, Naggert JK, North MA, Nishina PM (1996) A candidate gene for the mouse mutation tubby. Nature 380, 534-538
7. Ohlemiller KK, Hughes RM, Mosinger-Olilvie J, Speck JD, Grosof DH, Silverman MS (1995) Cochlear and retinal degeneration in the tubby mouse. Neuroreport 6, 845-849
8. Rust S, Funke H, Assmann G (1993) Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection. Nucleic Acids Res 16, 3623-3629