Genetic and biochemical characterization of Saccharomyces cerevisiae mutants resistant to trifluoroleucine

Research in Microbiology

Volumn 148, Issue 7, 1997, Pages 613-623

  Casalone, E ^a,b   Fia, G ^a   Barberio, C ^a   Cavalieri, D ^a   Turbanti, L ^a   Polsinelli, M ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b UNIVERSITY OF CHIETI   (Italy)

Author keywords

Fermentation secondary metabolites; LEU4; Leucine; Leucine excretion; Resistance; Saccharomyces cerevisiae; Trifluoroleucine

Indexed keywords

LEUCINE;

ARTICLE; CONTROLLED STUDY; FERMENTATION; GENETIC ANALYSIS; METABOLISM; MUTANT; NEGATIVE FEEDBACK; NONHUMAN; PRIORITY JOURNAL; R FACTOR; SACCHAROMYCES CEREVISIAE;

EID: 0030778968     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(97)88085-0     Document Type: Article

References (28)

1. Ashida, S., Ichikawa, E., Suginami, K. & Imayasu, S. (1987), Isolation and application of mutants producing sufficient isoamyl acetate, a sake flavour component. Agric. Biol. Chem., 51, 2061-2065.
2. Äyräpää, T. (1971), Biosynthetic formation of higher alcohols by yeast. Dependence on the nitrogenous nutrient level of the medium. J. Inst. Brewing, 77, 266-276.
3. Baichwal, V.R., Cunnigham, T.S., Gatzek, P.R. & Kohlhaw, G.B. (1983), Leucine biosynthesis in yeast: identification of two genes (LEU4, LEU5) that affect α-isopropylmalate synthase activity and evidence that LEU1 and LEU2 gene expression is controlled by α-isopropylmalate and the product of a regulatory gene. Curr. Genet., 7, 369-377.
4. Bradford, M.M. (1976), A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
5. Bussey, H. & Umbarger, H.E. (1970), Biosynthesis of the branched-chain amino acids in yeasts: a trifluoroleucine-resistant mutant with altered regulation of leucine uptake. J. Bacteriol., 103, 286-294.
6. Chang L.L., Gatzek P.R. & Kohlhaw G.B. (1985), Total delection of yeast LEU4: further evidence for a second α-isopropylmalate synthase and evidence for tight LEU4-MET4 linkage. Gene, 33, 333-339.
7. Derrick, S. & Large, P.J. (1993), Activities of the enzymes of the Ehrlich pathway and formation of the branched-chain alcohols in Saccharomyces cerevisiae and Candida utilis grown in continuous culture on valine or ammonium as sole nitrogen source. J. Gen. Microbiol., 139, 2783-2792.
8. Friden, P. & Shimmel, P. (1987), LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes. Mol. Cell. Biol., 7, 2708-2717.
9. Henschke, P.A. & Jiranek, V. (1993), Yeast-metabolism of nitrogen compounds, in "Wine microbiology and biotechnology" (G.H. Fleet) (pp. 77-164). Harwood Academic Publishers, Chur.
10. Hsu, Y.P., Kohlhaw, G.B. & Niederberger, P. (1982), Evidence that α-isopropylmalate synthase of Saccharomyces cerevisiae is under the "general" control of amino acid biosynthesis. J. Bacteriol., 150, 969-972.
11. Hu, Y. & Kohlhaw, G.B. (1995), Additive activation of yeast LEU4 transcription by multiple cis elements. J. Biol. Chem., 270, 5270-5275.
12. Martínez-Force, E. & Benítez, T. (1992), Selection of amino-acid overproducer yeast mutants. Curr. Genet., 21, 191-196.
13. Martínez-Force, E. & Benítez, T. (1993), Regulation of aspartate-derived amino acid biosynthesis in the yeast Saccharomyces cerevisiae. Curr. Microbiol., 26, 313-322.
14. Martínez-Force, E. & Benítez, T. (1994), Amino acid overproduction and catabolic pathway regulation in Saccharomyces cerevisiae. Biotechnol. Prog., 10, 372-376.
15. Mikkelsen, J.D. Sigsgaard, P., Olsen, A., Erdal, K., Kielland-Brandt, M.C. & Petersen, J.G. (1979), Thiaiso-leucine resistant mutants in Saccharomyces carlsbergensis increase the content of D-amyl alcohol in beer. Carlsberg Res. Commun., 51, 158-160.
16. Mortimer., R.K. & Hawthorne, D.C. (1969), in "The yeasts", Vol. I. (A.H. Rose & J.S. Harrison) (pp. 289-310) Academic Press, New York, London.
17. Mountain, H.A., Byström, A.S. & Korch, C. (1993), The general amino acid control regulates MET4, which encodes a methionine-pathway-specific transcriptional activator of Saccharomyces cerevisiae. Mol. Microbiol., 7, 215-228.
18. Nykänen, L. (1986), Formation and occurence of flavor compounds in wine and distilled beverages. Am. J. Enol. Viticol., 37, 84-96.
19. Peters, M.H., Beltzer, J.P. & Kohlhaw, G.B. (1990), Expression of the yeast LEU4 gene is subject to four different modes of control. Arch. Biochem. Biophys., 276, 294-298.
20. Ramos, C. & Calderon, I.L. (1992), Overproduction of threonine by Saccharomyces cerevisiae mutants resistant to hydroxynorvaline. Appl. Environ. Microbiol., 58, 1677-1682.
21. Romano, P., Suzzi, G., Turbanti, L. & Polsinelli, M. (1994), Acetaldehyde production in Saccharomyces cerevisiae wine yeasts. FEMS Microbiol. Lett., 118, 213-218.
22. nd ed. Cold Spring Harbor Laboratory, New York.
23. Rous, C.V. & Snow, R. (1983), Reduction of high alcohols by fermentation with leucine-auxotrophic mutant of wine yeast. J. Inst. Brewing, 89, 274-278.
24. Satyanarayana, T., Umbarger, H.E. & Lindegren, G. (1968), Biosynthesis of branched-chain amino acids in yeast: regulation of leucine biosynthesis in prototrophic and leucine auxotrophic strains. J. Bacteriol., 96, 2018-2024.
25. Sherman, F., Fink, G.R. & Hicks, J.B. (1986), in "Laboratory course manual for methods in yeast genetics". Cold Spring Harbor Laboratory, New York.
26. Ulm, E.H., Böhme, R. & Kohlhaw, G. (1972), α-isopropylmalate synthase from yeast: purification, kinetic studies, and effect of ligands on stability. J. Bacteriol., 110, 1118-1126.
27. Watanabe, M., Fukuda, K., Asano, K. & Ohta, S. (1990), Mutants of baker's yeasts producing a large amount of isomyl alcohol or isomyl alcohol, flavour components of bread. Appl. Microbiol. Biotechnol., 34, 154-159.
28. Yoshikawa, S., Oguri, I., Kondo, K., Fuzukawa, M., Shimisaka, M. & Okazaki, M. (1995), Enhanced formation of isoamyl alcohol in Zygosaccharomyces rouxii due to elimination of feedback inhibition of α-isopropylmalate synthase. FEMS Microbiol. Lett., 127, 139-143.