Genomic targeting of a bicistronic DNA fragment by Cre-mediated site-specific recombination

Gene

Volumn 203, Issue 2, 1997, Pages 209-216

  F Kolb, Andreas ^a,b   Siddell, Stuart G ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b HANNAH RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Cloning vectors; Genetic engineering; loxP site; Transcriptional interference; Transgenic animals

Indexed keywords

DNA FRAGMENT;

ANIMAL CELL; ARTICLE; BACTERIOPHAGE P1; CONTROLLED STUDY; DNA RECOMBINATION; GENE TARGETING; GENETIC ENGINEERING; GENOME; MARKER GENE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BACTERIOPHAGE P1; BLOTTING, SOUTHERN; CATS; CELL LINE; CLONING, MOLECULAR; DNA; GENE TARGETING; GENES, REPORTER; INTEGRASES; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; TRANSFECTION; VIRAL PROTEINS;

EID: 0030778619     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00515-5     Document Type: Article

References (15)

1. Albert H., Dale E.C., Lee E., Ow D.W. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J. 7:1995;649-659.
2. Baubonis W., Sauer B. Genomic targeting with purified Cre recombinase. Nucleic Acids Res. 21:1993;2025-2029.
3. Bergemann J., Kühlcke K., Fehse B., Ratz I., Ostertag W., Lother H. Excision of specific DNA-sequences from integrated retroviral vectors via site-specific recombination. Nucleic Acids Res. 23:1995;4451-4456.
4. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
5. Chen C., Okayama H. High efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7:1987;2745-2752.
6. Fukushige S., Sauer B. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA. 89:1992;7905-7909.
7. Gu H., Zou Y.R., Rajewsky K. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1993;1155-1164.
8. Jaenisch R. Transgenic animals. Science. 240:1988;1468-1474.
9. Kanegae Y., Lee G., Sato Y., Tanaka M., Nakai M., Sakaki T., Sugano S., Saito I. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res. 23:1995;3816-3821.
10. Kolb A.F., Gunzburg W.H., Albang R., Brem G., Erfle V., Salmons B. Negative regulatory element in the mammary specific whey acidic protein promoter. J. Cell. Biochem. 56:1994;245-261.
11. Kolb A.F., Siddell S.G. Genomic targeting with an MBP-Cre fusion protein. Gene. 183:1996;53-60.
12. Orlandi R., Güssow D.H., Jones P.T., Winter G. Cloning immunoglobulin domains for expression by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA. 86:1989;3833-3837.
13. Sauer B., Henderson N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol. 2:1990;441-449.
14. Selden R.F., Howie K.B., Rowe M.E., Goodman H.M., Moore D.D. Human growth hormone as a reporter gene in regulation studies employing transient gene expression. Mol. Cell. Biol. 6:1986;3173-3179.
15. Smith A.J., De Sousa M.A., Kwabi-Addo B., Heppel-Parton A., Impey H., Rabbits P. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nature Genet. 9:1995;376-385.