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4
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0025946883
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L. S. Schlesinger and M. A. Horwitz, J. Immunol. 147, 1983 (1991); L. S. Schlesinger, C. G. Bellinger-Kawahara, N. R. Payne, M. A. Horwitz, ibid. 144, 277 (1990).
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Schlesinger, L.S.1
Horwitz, M.A.2
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5
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0025216480
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L. S. Schlesinger and M. A. Horwitz, J. Immunol. 147, 1983 (1991); L. S. Schlesinger, C. G. Bellinger-Kawahara, N. R. Payne, M. A. Horwitz, ibid. 144, 277 (1990).
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Schlesinger, L.S.1
Bellinger-Kawahara, C.G.2
Payne, N.R.3
Horwitz, M.A.4
-
8
-
-
1842393006
-
-
note
-
+ serum, North American Biologicals, Miami, FL) or equine serum as described above or by batch mode. Protein eluted with HBSS, 0.1 M α-methyl mannoside was exchanged into phosphate-buffered saline (PBS) and used in the experiments at ∼0.15 mg/ml.
-
-
-
-
9
-
-
1842389090
-
-
data not shown
-
J. S. Schorey et al., data not shown.
-
-
-
Schorey, J.S.1
-
10
-
-
1842390041
-
-
note
-
2-terminus of human C2a is shown in bold. Abbreviations for the amino acid residues: A, Ala; C, Cys; D, Asp; G, Gly; H, His; I, Ile; K, Lys; L, Leu; N, Asn; Q, GIn; R, Arg; S, Ser; and Y, Tyr.
-
-
-
-
11
-
-
0013870426
-
-
G. Sitomer, R. M. Stroud, M. M. Mayer, Immunochemistry 3, 57 (1966); H. J. Muller-Eberhard, M. J. Polley, M. A. Calcott, J. Exp. Med. 125, 359 (1967).
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(1966)
Immunochemistry
, vol.3
, pp. 57
-
-
Sitomer, G.1
Stroud, R.M.2
Mayer, M.M.3
-
12
-
-
0014051320
-
-
G. Sitomer, R. M. Stroud, M. M. Mayer, Immunochemistry 3, 57 (1966); H. J. Muller-Eberhard, M. J. Polley, M. A. Calcott, J. Exp. Med. 125, 359 (1967).
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J. Exp. Med.
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, pp. 359
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Muller-Eberhard, H.J.1
Polley, M.J.2
Calcott, M.A.3
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13
-
-
0013904709
-
-
R. M. Stroud, M. M. Mayer, J. A. Miller, A. T. McKenzie, Immunochemistry 3, 163 (1966); M. J. Polley and H. J. Muller-Eberhard, J. Exp. Med. 128, 533 (1968).
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Immunochemistry
, vol.3
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Stroud, R.M.1
Mayer, M.M.2
Miller, J.A.3
McKenzie, A.T.4
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14
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-
0014330738
-
-
R. M. Stroud, M. M. Mayer, J. A. Miller, A. T. McKenzie, Immunochemistry 3, 163 (1966); M. J. Polley and H. J. Muller-Eberhard, J. Exp. Med. 128, 533 (1968).
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Polley, M.J.1
Muller-Eberhard, H.J.2
-
15
-
-
1842393005
-
-
note
-
To make the protein A-anti-C2, we incubated 0.05 ml of the rabbit polyclonal serum against human C2, which cross-reacts with equine C2, with 0.05 ml of packed protein A-Sepharose (Sigma) in 0.9 ml of PBS for 2 hours at 4°C. The protein A-Sepharose with coupled antibody was washed twice with PBS and resuspended in 0.1 ml of PBS. Approximately 0.2 mg of Con A-fractionated proteins from heat-treated equine serum or human serum (obtained from a mixed lymphocyte reaction) in PBS was incubated with protein A-Sepharose or protein A-Sepharose with bound anti-C2 for 3 hours at 4°C. The protein A was subsequently removed and the treated samples tested for the ability to support M. avium invasion of human monocyte-derived macrophages. No detectable C2 or C2 fragments were observed in the anti-C2-treated samples by protein immunoblot analysis with goat anti-C2 (human).
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-
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17
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0027137823
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R. W. Stokes, J. D. Haidl, W. A. Jefferies, D. P. Speert, J. Immunol. 151, 7067 (1993); L. S. Schlesinger, ibid. 150, 2920 (1993).
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J. Immunol.
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, pp. 7067
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Stokes, R.W.1
Haidl, J.D.2
Jefferies, W.A.3
Speert, D.P.4
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18
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-
0027173039
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-
R. W. Stokes, J. D. Haidl, W. A. Jefferies, D. P. Speert, J. Immunol. 151, 7067 (1993); L. S. Schlesinger, ibid. 150, 2920 (1993).
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Schlesinger, L.S.1
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19
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0018884086
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F. S. Cole, W. J. Matthews Jr., J. T. Marino, D. J. Gash, H. R. Colten, ibid. 125, 1120 (1980); H. U. Schorlemmer, D. Bitter-Suermann, A. C. Allison, Immunology 32, 929 (1977) ; H. U. Schorlemmer and A. C. Allison, ibid. 31, 781 (1976).
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, pp. 1120
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Cole, F.S.1
Matthews Jr., W.J.2
Marino, J.T.3
Gash, D.J.4
Colten, H.R.5
-
20
-
-
0017744558
-
-
F. S. Cole, W. J. Matthews Jr., J. T. Marino, D. J. Gash, H. R. Colten, ibid. 125, 1120 (1980); H. U. Schorlemmer, D. Bitter-Suermann, A. C. Allison, Immunology 32, 929 (1977) ; H. U. Schorlemmer and A. C. Allison, ibid. 31, 781 (1976).
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Immunology
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, pp. 929
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Schorlemmer, H.U.1
Bitter-Suermann, D.2
Allison, A.C.3
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21
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0017088678
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F. S. Cole, W. J. Matthews Jr., J. T. Marino, D. J. Gash, H. R. Colten, ibid. 125, 1120 (1980); H. U. Schorlemmer, D. Bitter-Suermann, A. C. Allison, Immunology 32, 929 (1977) ; H. U. Schorlemmer and A. C. Allison, ibid. 31, 781 (1976).
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, pp. 781
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Schorlemmer, H.U.1
Allison, A.C.2
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22
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0019447922
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J. Brostoff, L. Lenzini, P. Rottoli, L. Rottoli, Tubercle 62, 169 (1981); R. I. Carr et al., Clin. Exp. Immunol. 39, 562 (1980).
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Tubercle
, vol.62
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Brostoff, J.1
Lenzini, L.2
Rottoli, P.3
Rottoli, L.4
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23
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0018837275
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J. Brostoff, L. Lenzini, P. Rottoli, L. Rottoli, Tubercle 62, 169 (1981); R. I. Carr et al., Clin. Exp. Immunol. 39, 562 (1980).
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Carr, R.I.1
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24
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0025345833
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K. S. S. Sai-Baba, K. D. Moudgil, R. C. Jain, L. M. Srivastava, Tubercule 71, 103 (1990).
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Sai-Baba, K.S.S.1
Moudgil, K.D.2
Jain, R.C.3
Srivastava, L.M.4
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25
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0343555026
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I. H. Lepow, G. B. Naff, E. W. Todd, J. Pensky, C. F. Hinz, J. Exp. Med. 117, 983 (1963).
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Lepow, I.H.1
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Hinz, C.F.5
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27
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-
1842305264
-
-
note
-
2 in 0.2 ml of RPMI, 0.5% human serum albumin (infection media). The cells were washed with PBS, fixed with acetone/methanol (1:1), and stained for pathogenic mycobacteria with a TB Fluorescent Stain Kit O (Becton Dickinson, Cockney, MD). Macrophages infected with M. smegmatis, M. vaccae, and M. phlei were fixed in 2.5% glutaraldehyde and stained with acridine orange. The number of macrophages containing attached and internalized mycobacteria was scored visually with a Nikon Microphot-FX fluorescent microscope. The number of macrophages with associated mycobacteria per 100 cells is given as percent infected. For some experiments, the data are presented as the mean ± SD for 300 macrophages counted and represent variability within a given experimental chamber well. Defining the number of macrophages infected with organisms other then mycobacteria was done by visual inspection with a Zeiss Axioskop microscope.
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28
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1842321489
-
-
note
-
2+ with C1 and C4. After the final 2-hour incubation, EA were removed by centrifugation, and 0.1 ml of the supernatant was tested for the ability to support M. avium invasion of human monocyte-derived macrophages.
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-
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29
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0027215583
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T. I. Roach, C. H. Barton, D. Chatterjee, J. M. Blackwell, J. Immunol. 150, 1886 (1993).
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Roach, T.I.1
Barton, C.H.2
Chatterjee, D.3
Blackwell, J.M.4
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30
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1842384142
-
-
note
-
-/- mice were obtained through the eye orbital. The blood was allowed to clot for 30 min at 25°C and then 30 min at 4°C. The serum was cleared of clotted blood by centrifugation and immediately treated with a bovine serum albumin (BSA)-anti-BSA immune complex for 1 hour at 37°C. The immune complex was removed by centrifugation and the serum incubated at 56°C for 30 min to inactivate the complement pathways and stored at -70°C until needed.
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32
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0025879691
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H. D. Gresham, I. L. Graham, D. C. Anderson, E. J. Brown, J. Clin. Invest. 88, 588 (1991).
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Gresham, H.D.1
Graham, I.L.2
Anderson, D.C.3
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33
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0019052285
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R. A. Hoffman, P. C. Kung, W. P. Hansen, G. Goldstein, Proc. Natl. Acad. Sci. U.S.A. 77, 4914 (1980).
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Hoffman, R.A.1
Kung, P.C.2
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34
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0025666501
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0024506758
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H. D. Gresham, J. L. Goodwin, P. M. Allen, D. C. Anderson, E. J. Brown, ibid. 108, 1935 (1989).
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Gresham, H.D.1
Goodwin, J.L.2
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Anderson, D.C.4
Brown, E.J.5
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36
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1842381349
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note
-
We thank C. Hammer (NIH) for polyclonal anti-C2 and D. Russell (Washington University) for the L. mexicana and L. monocytogenes. Supported in part by NIH grant AI33348. J.S.S. is an American Foundation for Urologic Disease-Dornier Medical System Research Scholar.
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