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Volumn 277, Issue 5329, 1997, Pages 1091-1093

A macrophage invasion mechanism of pathogenic mycobacteria

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL INVASION; COMPLEMENT ACTIVATION; MACROPHAGE ACTIVATION; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; OPSONIZATION; PATHOGENICITY; PRIORITY JOURNAL;

EID: 0030777141     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5329.1091     Document Type: Article
Times cited : (153)

References (36)
  • 4
    • 0025946883 scopus 로고
    • L. S. Schlesinger and M. A. Horwitz, J. Immunol. 147, 1983 (1991); L. S. Schlesinger, C. G. Bellinger-Kawahara, N. R. Payne, M. A. Horwitz, ibid. 144, 277 (1990).
    • (1991) J. Immunol. , vol.147 , pp. 1983
    • Schlesinger, L.S.1    Horwitz, M.A.2
  • 8
    • 1842393006 scopus 로고    scopus 로고
    • note
    • + serum, North American Biologicals, Miami, FL) or equine serum as described above or by batch mode. Protein eluted with HBSS, 0.1 M α-methyl mannoside was exchanged into phosphate-buffered saline (PBS) and used in the experiments at ∼0.15 mg/ml.
  • 9
    • 1842389090 scopus 로고    scopus 로고
    • data not shown
    • J. S. Schorey et al., data not shown.
    • Schorey, J.S.1
  • 10
    • 1842390041 scopus 로고    scopus 로고
    • note
    • 2-terminus of human C2a is shown in bold. Abbreviations for the amino acid residues: A, Ala; C, Cys; D, Asp; G, Gly; H, His; I, Ile; K, Lys; L, Leu; N, Asn; Q, GIn; R, Arg; S, Ser; and Y, Tyr.
  • 14
    • 0014330738 scopus 로고
    • R. M. Stroud, M. M. Mayer, J. A. Miller, A. T. McKenzie, Immunochemistry 3, 163 (1966); M. J. Polley and H. J. Muller-Eberhard, J. Exp. Med. 128, 533 (1968).
    • (1968) J. Exp. Med. , vol.128 , pp. 533
    • Polley, M.J.1    Muller-Eberhard, H.J.2
  • 15
    • 1842393005 scopus 로고    scopus 로고
    • note
    • To make the protein A-anti-C2, we incubated 0.05 ml of the rabbit polyclonal serum against human C2, which cross-reacts with equine C2, with 0.05 ml of packed protein A-Sepharose (Sigma) in 0.9 ml of PBS for 2 hours at 4°C. The protein A-Sepharose with coupled antibody was washed twice with PBS and resuspended in 0.1 ml of PBS. Approximately 0.2 mg of Con A-fractionated proteins from heat-treated equine serum or human serum (obtained from a mixed lymphocyte reaction) in PBS was incubated with protein A-Sepharose or protein A-Sepharose with bound anti-C2 for 3 hours at 4°C. The protein A was subsequently removed and the treated samples tested for the ability to support M. avium invasion of human monocyte-derived macrophages. No detectable C2 or C2 fragments were observed in the anti-C2-treated samples by protein immunoblot analysis with goat anti-C2 (human).
  • 18
    • 0027173039 scopus 로고
    • R. W. Stokes, J. D. Haidl, W. A. Jefferies, D. P. Speert, J. Immunol. 151, 7067 (1993); L. S. Schlesinger, ibid. 150, 2920 (1993).
    • (1993) J. Immunol. , vol.150 , pp. 2920
    • Schlesinger, L.S.1
  • 20
    • 0017744558 scopus 로고
    • F. S. Cole, W. J. Matthews Jr., J. T. Marino, D. J. Gash, H. R. Colten, ibid. 125, 1120 (1980); H. U. Schorlemmer, D. Bitter-Suermann, A. C. Allison, Immunology 32, 929 (1977) ; H. U. Schorlemmer and A. C. Allison, ibid. 31, 781 (1976).
    • (1977) Immunology , vol.32 , pp. 929
    • Schorlemmer, H.U.1    Bitter-Suermann, D.2    Allison, A.C.3
  • 21
    • 0017088678 scopus 로고
    • F. S. Cole, W. J. Matthews Jr., J. T. Marino, D. J. Gash, H. R. Colten, ibid. 125, 1120 (1980); H. U. Schorlemmer, D. Bitter-Suermann, A. C. Allison, Immunology 32, 929 (1977) ; H. U. Schorlemmer and A. C. Allison, ibid. 31, 781 (1976).
    • (1976) Immunology , vol.31 , pp. 781
    • Schorlemmer, H.U.1    Allison, A.C.2
  • 23
    • 0018837275 scopus 로고
    • J. Brostoff, L. Lenzini, P. Rottoli, L. Rottoli, Tubercle 62, 169 (1981); R. I. Carr et al., Clin. Exp. Immunol. 39, 562 (1980).
    • (1980) Clin. Exp. Immunol. , vol.39 , pp. 562
    • Carr, R.I.1
  • 27
    • 1842305264 scopus 로고    scopus 로고
    • note
    • 2 in 0.2 ml of RPMI, 0.5% human serum albumin (infection media). The cells were washed with PBS, fixed with acetone/methanol (1:1), and stained for pathogenic mycobacteria with a TB Fluorescent Stain Kit O (Becton Dickinson, Cockney, MD). Macrophages infected with M. smegmatis, M. vaccae, and M. phlei were fixed in 2.5% glutaraldehyde and stained with acridine orange. The number of macrophages containing attached and internalized mycobacteria was scored visually with a Nikon Microphot-FX fluorescent microscope. The number of macrophages with associated mycobacteria per 100 cells is given as percent infected. For some experiments, the data are presented as the mean ± SD for 300 macrophages counted and represent variability within a given experimental chamber well. Defining the number of macrophages infected with organisms other then mycobacteria was done by visual inspection with a Zeiss Axioskop microscope.
  • 28
    • 1842321489 scopus 로고    scopus 로고
    • note
    • 2+ with C1 and C4. After the final 2-hour incubation, EA were removed by centrifugation, and 0.1 ml of the supernatant was tested for the ability to support M. avium invasion of human monocyte-derived macrophages.
  • 30
    • 1842384142 scopus 로고    scopus 로고
    • note
    • -/- mice were obtained through the eye orbital. The blood was allowed to clot for 30 min at 25°C and then 30 min at 4°C. The serum was cleared of clotted blood by centrifugation and immediately treated with a bovine serum albumin (BSA)-anti-BSA immune complex for 1 hour at 37°C. The immune complex was removed by centrifugation and the serum incubated at 56°C for 30 min to inactivate the complement pathways and stored at -70°C until needed.
  • 36
    • 1842381349 scopus 로고    scopus 로고
    • note
    • We thank C. Hammer (NIH) for polyclonal anti-C2 and D. Russell (Washington University) for the L. mexicana and L. monocytogenes. Supported in part by NIH grant AI33348. J.S.S. is an American Foundation for Urologic Disease-Dornier Medical System Research Scholar.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.