A nested PCR method to detect Listeria monocytogenes in artificially contaminated blood specimens

Research in Microbiology

Volumn 148, Issue 6, 1997, Pages 485-490

  Cocolin, L ^a   Manzano, M ^a   Cantoni, C ^a   Comi, G ^a  

^a NONE

Author keywords

Bacteraemia; Diagnosis; Iap gene; Listeria monocytogenes; Listeriosis; PCR

Indexed keywords

ETHIDIUM BROMIDE;

ANIMAL CELL; ARTICLE; BACTERIUM CONTAMINATION; BLOOD SAMPLING; GEL ELECTROPHORESIS; GENE AMPLIFICATION; LISTERIA MONOCYTOGENES; LISTERIOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAT;

ANIMALIA; BACTERIA (MICROORGANISMS); LISTERIA MONOCYTOGENES; LISTERIOSIS MONOCYTOGENES;

EID: 0030773481     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(97)88346-5     Document Type: Article

References (17)

1. Bubert, A., Kohler, S. & Goebel, W. (1991), The homologous and heterologous regions within the iap gene allow genus-and species-specific identification of Listeria spp. by polymerase chain reaction. Appl. Environ. Microbiol., 58, 2625-2632.
2. Comi, G., Cocolin, L., Cantoni, C. & Manzano, M. (1997), A RE-PCR method to distinguish Listeria monocytogenes serovars. FEMS-Immunol. Med. Microbiol. (in press).
3. Czajka, J. & Batt, C.A. (1994), Verification of causal relationships between Listeria monocytogenes isolates implicated in food-borne outbreaks of listeriosis by randomly amplified polymorphic DNA patterns. J. Clin. Microbiol., 32, 1280-1287.
4. Durand, M.L., Calderwood, S.B., Weber, D.J., Miller, S.I., Southwick, F.S., Caviness, V.S. & Swartz, M.N. (1993), Acute bacterial meningitis in adults - A review of 493 episodes. N. Engl. J. Med., 328, 21-28.
5. Farber, J.M., Carter, A.O., Varughese, P.V., Ashton, F.E. & Ewan, E.P. (1990), Listeriosis traced to the consumption of alfalfa tablets and soft cheese. N. Engl. J. Med., 322, 338.
6. Fleming, D.W., Cochi, S.L., MacDonald, K.L., Brondum, J., Hayes, P.S., Plikaytis, B.D., Holmes, M.B., Audurier, A., Broome, C.V. & Reingold, A.L. (1985), Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N. Engl. J. Med., 312, 404-407.
7. Gellin, B.C. & Broome, C.V. (1989), Listeriosis. J. Am. Med. Assoc., 261, 1313-1320.
8. Jalon, K., Sahli, R. & Bille, J. (1992), Development of polymerase chain reaction assays for detection of Listeria monocytogenes in clinical cerebrospinal fluid samples. J. Clin. Microbiol., 30, 1931-1936.
9. Kohler, S., Leimeister-Wachter, M., Chakraborty, T., Lottspeich, F. & Goebel, W. (1990), The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes. Infect. Immun., 58, 1943-1950.
10. Maniatis, T., Fritsch, E.F. & Sambrook, J. (1982), Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
11. Manzano, M., Cocolin, L., Ferroni, P., Cantoni, C. & Comi, G. (1997a), A simple PCR method to detect Listeria monocytogenes from meat. J. Sci. Food Agric., 73.
12. Manzano, M., Cocolin, L., Cantoni, C. & Comi, G. (1997b), Detect of Listeria monocytogenes from milk and cheese by a single step PCR. Mol. Biotechnol. (in press).
13. Panaccio, M. & Lew, A.M. (1994), Direct PCR from whole blood using formamide and low temperatures, in "PCR technology, current innovations" (H.G. Griffin & A.M. Griffin) (pp. 151-157). CRC Press, N.W., Boca Raton, Florida.
14. Pershing, D.H. (1993), In vitro nucleic acid amplification techniques, in "Diagnostic molecular microbiology: principles and applications" (D.H. Pershing, T.F. Smiths, F.C. Tenover & T.J. White) (pp. 51-87). American Society for Microbiology, Washington, D.C.
15. Rocourt, J. (1994), Listeria monocytogenes: the state of the science. Dairy Food Environ. Sanitat., 14, 70-82.
16. Schwartz, B., Ciesielski, C.A., Broome, C.V., Gaventa, S., Brown, G.R., Gellin, B.G., Hightower, A.W. & Mascola, L. (1988), Association of sporadic listeriosis with consumption of uncooked hot dogs and undercooked chicken. Lancet, ii, 779-782.
17. Song, J.H., Cho, H., Park, M.Y., Na, D.S., Moon, H.B. & Pai, C.H. (1993), Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction. J. Clin. Microbiol., 31, 1439-1443.