Identification of proteins that interact with a protein of interest: Applications of the yeast two-hybrid system

Molecular and Cellular Biochemistry

Volumn 172, Issue 1-2, 1997, Pages 67-79

  Gietz, R Daniel ^a,b   Triggs Raine, Barbara ^b   Robbins, Anne ^a   Graham, Kevin C ^a   Woods, Robin A ^c  

^a Department of Human Genetics ^*  (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

^c UNIVERSITY OF WINNIPEG   (Canada)

Author keywords

False positive elimination; Protein interaction; Screening; Two hybrid system; Yeast

Indexed keywords

BETA GALACTOSIDASE; COMPLEMENTARY DNA; HYBRID PROTEIN;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; GENE TECHNOLOGY; MOLECULAR GENETICS; NONHUMAN; PLASMID; PROTEIN PROTEIN INTERACTION; REPORTER GENE; YEAST CELL; YEAST TWO HYBRID SYSTEM;

BETA-GALACTOSIDASE; CLONING, MOLECULAR; FALSE POSITIVE REACTIONS; FUNGAL PROTEINS; GENE LIBRARY; GENES, REPORTER; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSFECTION;

EID: 0030773139     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006859319926     Document Type: Article

References (21)

1. Fields S, Song O: A novel genetic system to detect protein-protein interactions. Nature 340: 45-246, 1989
2. Chien C, Bartel PL, Fields S: The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci USA 88: 9578 9582, 1991
3. Durfee T, Becherer K, Chen R-L, Yeh SH, Yang Y, Kilburn AK, Lee WH, Elledge SJ: The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Gene & Dev 7: 55-569, 1993
4. Vojtek AB, Hollenberg SM, Cooper JA: Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell 74: 205-214, 1993
5. Ito H, Fukuda Y, Murata K, Kimura A: Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153: 163-168, 1983
6. Gietz RD, Woods RA: High efficiency transformation of yeast with lithium acetate. In: JR Johnston (ed). Molecular Genetics of Yeast: A Practical Approach. Oxford University Press, Oxford, 1994, pp 121-134
7. Breeden L, Nasmyth K: Regulation of the yeast HO gene. Cold Spring Symposia on Quantitative Biology 50: 643-650, 1985
8. Bartel PL, Fields S: Analyzing protein-protein interactions using the two-hybrid system. Methods Enzymol 254: 241-263, 1995
9. Miller JH: Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1972
10. Kolodziej P, Young RA: Epitope tagging and protein surveillance. Methods Enzymol 194: 508-519, 1991
11. Bartel P, Chien C-T, Sternglanz R, Fields S: Elimination of false positives that arise in using the two-hybrid system. Biotechniques 14: 920-24, 1993
12. Hoffman CS, Winston F: A ten minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of E. coli. Gene 57: 267-272, 1987
13. Dower WJ, Miller JF, Ragsdale CW: High efficiency transformation of E. coli by high voltage electroporation. Nucl Acids Res 16: 6127-6145, 1988
14. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1989
15. Hanahan D: Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166: 557-580, 1983
16. Birnboim HC, Doly J: A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acids Res 7: 1513-1517, 1979
17. Burke DT, Olson MV: Preparation of clone libraries in yeast artificial chromosome vectors. Methods Enzymol 194: 252-270, 1991
18. Bardwell L, Cooper AJ, Friedberg EC: Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro. Mol Cell Biol 12: 3041-3049, 1992
19. Osborne MA, Dalton S, Kochan JP: The yeast tri-brid system-genetic detection of frans-phosphorylated ITAM-SH2-interactions. Biotechnology 13: 1474-1478, 1995
20. Harper JW, Adami G, Wei N, Keyomarsi K, Elledge SJ: The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kineses. Cell 75: 805-816, 1993
21. Bartel PL, Chien C-T, Sternglanz R, Fields S: Using the two-hybrid system to detect protein-protein interactions. In: DA Hartley (ed). Cellular Interactions in Development: A Practical Approach. Oxford University Press, Oxford, 1993b, pp 153-179