Enhanced cytokine detection by a novel cell culture-based ELISA

Journal of Immunoassay

Volumn 18, Issue 4, 1997, Pages 371-388

  Shankar, Gopi ^a   Cohen, Donald A ^a  

^a UNIVERSITY OF KENTUCKY MEDICAL CENTER   (United States)

Author keywords

Capture; Culture technique; Cytokines; ELISA; Enhanced detection; Enzyme linked immunosorbent assay

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; MOUSE; NONHUMAN; PROTEIN DETERMINATION; T LYMPHOCYTE;

EID: 0030772851     PISSN: 01971522     EISSN: None     Source Type: Journal    
DOI: 10.1080/01971529708005828     Document Type: Article

References (5)

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2. Powers, G.D., Abbas, A.K., and Miller, R.A. Frequencies of IL-2 and IL-4 secreting T cells in naive and antigen stimulated lymphocyte populations. J. Immunol. 1988; 140: 3352- 3357
3. Swain, S.L., McKenzie, D.T., Weinberg, A.D., and Hancock, W. Characterization of T helper 1 and 2 cell subsets in normal mice. Helper T cells responsible for IL-4 and IL-5 production are present as precursors that require priming before they develop into lymphokine-secreting cells. J. Immunol. 1988; 141: 3445-3455
4. Mosmann, T.R., Schumacher, J.H., Fiorentino, D.F., et al. Isolation of monoclonal antibodies specific for IL-4, IL-5, IL-6, and a new Th2-specific cytokine (IL-10), cytokine synthesis inhibitory factor, by using a solid phase radioimmunoadsorbent assay. J. Immunol. 1990; 145: 2938 - 2945
5. Sander, B., Hoiden, I., Andersson, U., Moller, E., and Abrams, J.S. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J. Immunol. Meth. 1993; 166: 201-214