An easily constructed pipette for pressure microinjections into the brain

Brain Research Bulletin

Volumn 44, Issue 2, 1997, Pages 199-203

  Kratskin, Igor L ^a   Yu, Xiaosong ^a   Doty, Richard L ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Biotin dextran amine; Pipette construction; Pressure microinjections

Indexed keywords

AMINE; BIOTIN; DEXTRAN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; INJECTION SITE; INTRACRANIAL PRESSURE; IONTOPHORESIS; MICROINJECTION; NERVE FIBER TRANSPORT; NONHUMAN; PIPETTE; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; TISSUE INJURY;

ANIMALS; BIOTIN; BRAIN; DEXTRANS; EQUIPMENT DESIGN; EVALUATION STUDIES; FLUORESCENT DYES; HISTOCYTOCHEMISTRY; INJECTIONS, JET; MICROINJECTIONS; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS;

EID: 0030772622     PISSN: 03619230     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0361-9230(97)00092-0     Document Type: Article

References (14)

1. Alheid G. F., Edwards S. B., Kitai S. T., Park M. R., Switzer R. C. Methods for delivering tracers. Heimer L., RoBards M. J. Neuroanatomical tract-tracing methods. 1981;91-116 Plenum Press, New York.
2. Bullier J., Henry G. H., Baker W. J. A simple device for injection of small calibrated amounts of horseradish peroxidase into cerebral cortex. J. Neurosci. Methods. 2:1980;47-49.
3. Fish S. E., Rhoades R. W. A method for making very small, quantifiable micropipette injections of axonal tracer substances. J. Neurosci. Res. 4:1981;291-297.
4. Lasek R., Joseph B. S., Whitlock D. G. Evaluation of a radiographic neuroanatomical tracing method. Brain Res. 8:1968;319-336.
5. Mesulam M.-M. Principles of horseradish peroxidase neurohistochemistry and their applications for tracing neural pathways - Axonal transport, enzyme histochemistry and light microscopic analysis. Mesulam M.-M. Tracing neural connections with horseradish peroxidase. 1982;1-151 John Wiley & Sons, Chichester.
6. Meyers D. E. R., Snow P. J. A new device for making multiple injections of small volumes of horseradish peroxidase into the spinal cord. J. Neurosci. Methods. 4:1981;343-346.
7. Paxinos G., Watson C. The rat brain in stereotaxic coordinates. 1986;Academic Press, New York.
8. Price P., Fisher A. W. F., Redstone P. A simple apparatus for the injection of small (nanoliter) volumes of horseradish peroxidase. Neurosci. Lett. 6:1977;21-25.
9. Rajakumar N., Elisevitch K., Flumerfelt B. A. Biotinylated dextran: A versatile anterograde and retrograde neuronal tracer. Brain Res. 607:1993;47-53.
10. Saper C. B. A simple inexpensive and reliable nanoliter syringe. Brain Res. Bull. 10:1983;403-405.
11. Shipley M. T. A simple, low cost hydraulic pressure device for making microinjections in the brain. Brain Res. Bull. 8:1982;237-239.
12. Veenman C. L., Reiner A., Honig M. G. Biotinylated dextran amine as an anterograde tracer for single- and double-labeling studies. J. Neurosci. Methods. 41:1992;239-254.
13. Wouterlood F. G., Jorritsma-Byham B. The anterograde neuroanatomical tracer biotinylated dextran-amine: Comparison with the tracer Phaseolus vulgaris-leucoagglutinin in preparations for electron microscopy. J. Neurosci. Methods. 48:1993;75-87.
14. Záborszky L., Carlsen J., Brashear H. R., Heimer L. Cholinergic and GABAergic afferents to the olfactory bulb in the rat with special emphasis on the projection neurons in the nucleus of the horizontal limb of the diagonal band. J. Comp. Neurol. 243:1986;488-509.