Reduced activity of the pyruvate dehydrogenase complex but not cytochrome c oxidase is associated with neuronal loss in the striatum following short-term forebrain ischemia

Brain Research

Volumn 772, Issue 1-2, 1997, Pages 23-28

  Zaidan, Emad ^a   Sims, Neil R ^a  

^a FLINDERS UNIVERSITY   (Australia)

Author keywords

Cytochrome c oxidase; Ischemia; Mitochondria; Neuronal death; Pyruvate dehydrogenase complex; Selective vulnerability

Indexed keywords

CYTOCHROME C OXIDASE; PYRUVATE DEHYDROGENASE COMPLEX;

ANIMAL TISSUE; ARTICLE; BRAIN ISCHEMIA; CELL SELECTION; CONTROLLED STUDY; CORPUS STRIATUM; ENZYME ACTIVITY; FOREBRAIN; MALE; NERVE CELL NECROSIS; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CELL DEATH; ELECTRON TRANSPORT COMPLEX IV; ISCHEMIC ATTACK, TRANSIENT; MALE; MITOCHONDRIA; NEURONS; PROSENCEPHALON; PYRUVATE DEHYDROGENASE COMPLEX; RATS; RATS, INBRED STRAINS;

EID: 0030771096     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(97)00833-0     Document Type: Article

References (24)

1. Abe J.K., Aoki M., Kogure K. Changes of mitochondrial DNA and heat shock protein gene expressions in gerbil hippocampus after transient forebrain ischemia. J. Cereb. Blood Flow Metab. 13:1993;773-780.
2. Almeida A., Allen K.L., Bates T.E., Clark J.B. Effect of reperfusion following cerebral ischaemia on the activity of the mitochondrial respiratory chain in the gerbil brain. J. Neurochem. 65:1995;1698-1703.
3. Bogaert Y.E., Rosenthal R.-E., Fiskum G. Post-ischemic inhibition of cerebral cortex pyruvate dehydrogenase. Free Radic. Biol. Med. 16:1994;811-820.
4. Chesselet M.-F., Gonzales C., Lin C.-S., Polsky K., Jin B.-K. Ischemic damage in the striatum of adult gerbils: relative sparing of somatostatinergic and cholinergic interneurons contrasts with loss of efferent neurons. Exp. Neurol. 110:1990;209-218.
5. Crain B.J., Westerkam W.D., Harrison A.H., Nadler J.V. Selective neuronal death after transient forebrain ischemia in the mongolian gerbil: a silver impregnation study. Neuroscience. 27:1988;387-402.
6. Dunkley P.R., Jarvie P.E., Heath J.W., Kidd G.J., Rostas J.A.P. A rapid method for isolation of synaptosomes on Percoll gradients. Brain Res. 372:1986;115-129.
7. Elnageh K.M., Gaitonde M.K. Effect of a deficiency of thiamine on brain pyruvate dehydrogenase: enzyme assay by three different methods. J. Neurochem. 51:1988;1482-1489.
8. Errede B., Kamen M.D., Hatefi Y. Preparation and properties of complex IV (ferrocytochrome c: oxygen oxidoreductase EC 1.9.3.1). Methods Enzymol. 53:1978;40-47.
9. Ginsberg M.D., Busto R. Rodent models of cerebral ischemia. Stroke. 20:1990;1627-1642.
10. Kristian T., Siesjo B. Calcium-related damage in ischemia. Life Sci. 59:1996;357-367.
11. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
12. Pulsinelli W.A., Brierley J.B., Plum F. Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann. Neurol. 11:1982;491-498.
13. Pulsinelli W.A., Duffy T.E. Regional energy balance in rat brain after transient forebrain ischemia. J. Neurochem. 40:1983;1500-1503.
14. Schmidt-Kastner R., Freund T.F. Selective vulnerability of the hippocampus in brain ischemia. Neuroscience. 40:1991;599-636.
15. Sims N.R. Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation. J. Neurochem. 55:1990;698-707.
16. Sims N.R. Selective impairment of respiration in mitochondria isolated from brain subregions following transient forebrain ischemia in the rat. J. Neurochem. 56:1991;1836-1844.
17. Sims N.R., Pulsinelli W.A. Altered mitochondrial respiration in selectively vulnerable brain subregions following transient forebrain ischemia in the rat. J. Neurochem. 49:1987;1367-1374.
18. Sims N.R., Zaidan E. Biochemical changes associated with selective neuronal death following short-term cerebral ischemia. Int. J. Biochem. Cell Biol. 27:1995;531-550.
19. R.R. Sokal, F.J. Rohlf, Biometry: the Principles and Practice of Statistics in Biological Research (2nd edition), W.H. Freeman, San Francisco, 1981, pp. 859.
20. Wagner K.R., Kleinholz M., Myers R.E. Delayed onset of neurologic deterioration following anoxia/ischemia coincides with appearance of impaired brain mitochondrial respiration and decreased cytochrome oxidase activity. J. Cereb. Blood Flow Metab. 10:1990;417-423.
21. Wharton D.C., Tzagoloff A. Cytochrome oxidase from beef heart mitochondria. Methods Enzymol. 10:1967;245-250.
22. 14C]glucose in brain subregions following transient forebrain ischemia in the rat. J. Neurochem. 55:1990;1882-1889.
23. Zaidan E., Sims N.R. Selective reductions in the activity of the pyruvate dehydrogenase complex in mitochondria isolated from brain subregions following forebrain ischemia in rats. J. Cereb. Blood Flow Metab. 13:1993;98-104.
24. Zaidan E., Sims N.R. The calcium content of mitochondria from brain subregions following short-term forebrain ischemia and recirculation in the rat. J. Neurochem. 63:1994;1812-1819.