Epidermal cell differentiation in Arabidopsis determined by a Myb homolog, CPC

Science

Volumn 277, Issue 5329, 1997, Pages 1113-1116

  Wada, Takuji ^a,b,c   Tachibana, Tatsuhiko ^b   Shimura, Yoshiro ^a,b,c   Okada, Kiyotaka ^a,b  

^a NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c BIOMOL ENG RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARABIDOPSIS; ARTICLE; BINDING SITE; CELL DIFFERENTIATION; EPIDERMIS CELL; GENE MUTATION; GENETIC TRANSCRIPTION; HAIR CELL; NONHUMAN; PLANT ROOT; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CELL DIFFERENTIATION; CROSSES, GENETIC; DNA-BINDING PROTEINS; GENES, PLANT; HOMEODOMAIN PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; ONCOGENES; PHENOTYPE; PLANT PROTEINS; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-MYB; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS;

ANIMALIA; ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0030770744     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5329.1113     Document Type: Article

References (27)

1. P. N. Benfey and J. W. Schiefelbein, Trends Genet. 10, 84 (1994); L. Dolan et al., Development 119, 71 (1993); C. van den Berg, V. Wilemsen, W. Hage, P. Weisbeek, B. Scheres, Nature 378, 62 (1995).
2. P. N. Benfey and J. W. Schiefelbein, Trends Genet. 10, 84 (1994); L. Dolan et al., Development 119, 71 (1993); C. van den Berg, V. Wilemsen, W. Hage, P. Weisbeek, B. Scheres, Nature 378, 62 (1995).
3. P. N. Benfey and J. W. Schiefelbein, Trends Genet. 10, 84 (1994); L. Dolan et al., Development 119, 71 (1993); C. van den Berg, V. Wilemsen, W. Hage, P. Weisbeek, B. Scheres, Nature 378, 62 (1995).
4. L. Dolan et al., Development 120, 2465 (1994).
5. M. E. Galway et al., Dev. Biol. 166, 740 (1994).
6. J. D. Massuci et al., Development 122, 1253 (1996).
7. W. G. Rerie, K. A. Feldmann, D. M. Marks, Genes Dev. 8, 1388 (1994).
8. A. M. Lloyd, V. Walbot, R. W. Davis, Science 258, 1773 (1992).
9. Arabidopsis (ecotype WS) T-DNA-tagged lines were made by the in planta method [S. S. Chang et al., Plant J. 5, 551 (1994)]. To observe the roots of plants in petri plates, we surface sterilized Arabidopsis seeds and grew them in medium described previously [K. Okada, Y. Shimura, Science 250, 274 (1990)]. Plants were incubated in a room under continuous illumination with white fluorescent light at 22°C.
10. Arabidopsis (ecotype WS) T-DNA-tagged lines were made by the in planta method [S. S. Chang et al., Plant J. 5, 551 (1994)]. To observe the roots of plants in petri plates, we surface sterilized Arabidopsis seeds and grew them in medium described previously [K. Okada, Y. Shimura, Science 250, 274 (1990)]. Plants were incubated in a room under continuous illumination with white fluorescent light at 22°C.
11. T. Wada, T. Tachibana, Y. Shimura, K. Okada, unpublished data.
12. 2 plants with the mutant phenotype carried the 4.9-kb band homozygously. This result indicated that the location of the T-DNA insertion and the cpc locus were tightly linked.
13. Mapping experiments with recombinant inbred lines were conducted by use of RFLP of Bam HI-digested genomic DNA [C. Lister and C. Dean, Plant J. 4, 745 (1993)]. The recombination frequency was calculated by the Map Maker program [E. S. Lander et al., Genomics 1, 174 (1987)].
14. Mapping experiments with recombinant inbred lines were conducted by use of RFLP of Bam HI-digested genomic DNA [C. Lister and C. Dean, Plant J. 4, 745 (1993)]. The recombination frequency was calculated by the Map Maker program [E. S. Lander et al., Genomics 1, 174 (1987)].
15. 6 recombinants) was screened with the genomic 5-kb Xba I-Bam HI fragment used as a probe.
16. Three genomic fragments were ligated to a binary vector, pARK5, and introduced into an agrobacterium strain, C58C1 [H. Anzai, K. Yoneyama, I. Yamaguchi, Nucleic Acids Res. 18, 1890 (1990)]. We then introduced them into Arabidopsis (ecotype WS) by the vacuum-infiltration method [N. Bechtold, J. Ellis, G. Pelletier, C. R. Acad. Sci. Paris Life Sci. 316, 1194 (1993)].
17. Three genomic fragments were ligated to a binary vector, pARK5, and introduced into an agrobacterium strain, C58C1 [H. Anzai, K. Yoneyama, I. Yamaguchi, Nucleic Acids Res. 18, 1890 (1990)]. We then introduced them into Arabidopsis (ecotype WS) by the vacuum-infiltration method [N. Bechtold, J. Ellis, G. Pelletier, C. R. Acad. Sci. Paris Life Sci. 316, 1194 (1993)].
18. B. Majello, L. C. Kenyon, R. Dalla-Favera, Proc. Natl. Acad. Sci. U.S.A. 83, 9636 (1986); K. Ogata et al., Cell 79, 639 (1994).
19. B. Majello, L. C. Kenyon, R. Dalla-Favera, Proc. Natl. Acad. Sci. U.S.A. 83, 9636 (1986); K. Ogata et al., Cell 79, 639 (1994).
20. J. Paz-Arez, D. Ghosal, U. Wieland, P. A. Peterson, H. Seadler, EMBO J. 6, 3353 (1987); E. Grotewold, P. Athma, T. Peterson, Proc. Natl. Acad. Sci. U.S.A. 88, 4587 (1991); K. C. Cone, S. M. Cocciolone, F. A. Burr, B. Burr, Plant Cell 5, 1795 (1993).
21. J. Paz-Arez, D. Ghosal, U. Wieland, P. A. Peterson, H. Seadler, EMBO J. 6, 3353 (1987); E. Grotewold, P. Athma, T. Peterson, Proc. Natl. Acad. Sci. U.S.A. 88, 4587 (1991); K. C. Cone, S. M. Cocciolone, F. A. Burr, B. Burr, Plant Cell 5, 1795 (1993).
22. J. Paz-Arez, D. Ghosal, U. Wieland, P. A. Peterson, H. Seadler, EMBO J. 6, 3353 (1987); E. Grotewold, P. Athma, T. Peterson, Proc. Natl. Acad. Sci. U.S.A. 88, 4587 (1991); K. C. Cone, S. M. Cocciolone, F. A. Burr, B. Burr, Plant Cell 5, 1795 (1993).
23. D. G. Oppenheimer, P. L. Herman, S. Sivakumaran, J. Esch, M. D. Marks, Cell 67, 483 (1991).
24. K. Noda, B. J. Glover, P. Linstead, C. Martin, Nature 369, 661 (1994).
25. The CPC cDNA clone was subcloned into the vector pMAT137-Hm [K. Matsuoka and K. Nakamura, Proc. Natl. Acad. Sci. U.S.A. 88, 834 (1991)] between Xba I and Kpn I sites under control of the 35S promoter and introduced into Arabidopsis (ecotype WS) following the method described in (12).
26. T. Wada, T. Tachibana, Y. Shimura, K. Okada, unpublished data.
27. We thank the Nottingham Arabidopsis Stock Centre (University of Nottingham, UK) for providing the seeds of gl2-1 and ttg-1 mutants; S. Ishiguro and other members of our laboratory for suggestions; D. M. Marks and J. W. Schiefelbein for discussions; and A. Kawai for making the transgenic Arabidopsis lines. Supported by a Grant-in-Aid for Scientific Research (A) number 08408031 and a Grant-in-Aid for Scientific Research on Priority Areas number 06278103 from the Japanese Ministry of Education, Science, Culture and Sports and by funds from the Joint Studies Program for Advanced Studies from the Science and Technology Agency of Japan.