Method for the simultaneous assay of initial velocities of lactate dehydrogenase isoenzymes following gel electrophoresis

Journal of Biochemical and Biophysical Methods

Volumn 34, Issue 3, 1997, Pages 167-175

  Yoshida, Masami ^a,b   Takakuwa, Yuichi ^a  

^a TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

^b SEITOKU UNIVERSITY   (Japan)

Author keywords

Assay; Electrophoresis; Lactate dehydrogenase; Lactate dehydrogenase isoenzymes

Indexed keywords

LACTATE DEHYDROGENASE ISOENZYME;

ANIMAL TISSUE; ARTICLE; CATTLE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME ASSAY; HEART; KINETICS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SKELETAL MUSCLE; TECHNIQUE;

ACRYLAMIDE; ACRYLAMIDES; ANIMALS; BUFFERS; CATTLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ISOENZYMES; KINETICS; L-LACTATE DEHYDROGENASE; MUSCLE, SKELETAL; MYOCARDIUM; SENSITIVITY AND SPECIFICITY;

ANIMALIA; BOS TAURUS;

EID: 0030769793     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(97)00012-2     Document Type: Article

References (19)

1. Fritz, P.J., Morrison, W.J., White E.L. and Vesell, E.S., Comparative study of methods for quantitation of lactate dehydrogenase isozymes, Anal. Biochem., 36 (1970) 443-453.
2. Shioya, M., Yanagisawa, M., Kawamura, T. and Kanno, T., Separation of lactate dehydrogenase isoenzymes on cellulose acetate, Jpn. J. Clin. Pathol. (Rinsho Byori), 19 (1971) 469-472.
3. Rosalki, S.B., Standardisation of isoenzyme assays with special reference to lactate dehydrogenase isoenzyme electrophoresis, Clin. Biochem., 7 (1974) 29-40.
4. Menon, M.P., Anderson, G. and Namblar, G.K., Determination of human serum lactate dehydrogenase isoenzymes by anion exchange chromatography, Anal. Chem., 55 (1983) 1385-1390.
5. 1 assay for myocardial infarction, Am. J. Clin. Pathol., 76 (1981) 426-429.
6. Vaidya, H.C., Porter, S.E., Landt, Y., Silva D.P., Dietzler D.N. and Ladenson, J.H., Quantification of lactate dehydrogenase-1 in serum with use of an M-subunit-specific monoclonal antibody, Clin. Chem., 34 (1988) 2410-2414.
7. 2 in serum as determined by using an inhibitor of the M-subunit, Clin. Chem., 31 (1985) 1175-1177.
8. Shirahase, Y., Watazu, Y., Kaneda, N., Uji, Y., Okabe, H. and Kannen, A., Specific assay of serum lactate dehydrogenase isoenzyme 1 by proteolysis with α-chymotrypsin and protein denaturation, Clin. Chem., 38 (1992) 2193-2196.
9. Pridgar, E.M., Leung, F.Y. and Henderson, A.R., Accuracy of lactate dehydrogenase isoenzyme estimations by a thin-layer agarose fluorescent technique: Experience with ternary and quaternary mixtures of purified human lactate dehydrogenase isoenzymes, Clin. Chem., 29 (1983) 2096-2099.
10. Marshall, T., Electrophoresis of serum isoenzymes and proteins following acute myocardial infarction, J. Chromatogr., 569 (1991) 323-345.
11. Lott, J.A., Serum enzyme determinations in the diagnosis of acute myocardial infarction: An update, Human Pathol., 15 (1984) 706-716.
12. Ramdeo, I.N. and Joshi, K.C., Serum lactic dehydrogenase and its isoenzymes in hepatic disorders, Am. J. Gastroenterol., 55 (1971) 459-465.
13. Kikuchi, Y., Hisano, A., Kuki, E., Hirata, J. and Nagata, I., Total lactate dehydrogenase and its isozymes in serum from patients with primary carcinoma of the ovary, Gynecol. Obstet. Invest., 31 (1991) 161-165.
14. Wieme, R.J. and Herpol, J.E., Origin of the lactate dehydrogenase isoenzyme pattern found in the serum of patients having primary muscular dystrophy, Nature, 194 (1962) 287-288.
15. McKenzie, D. and Henderson, A.R., Electrophoresis of lactate dehydrogenase isoenzymes, Clin. Chem., 29 (1983) 189-195.
16. Moses, G.C., Ross, M.L. and Henderson, A.R., Ten electrophoretic methods compared with a selected method for quantifying lactate dehydrogenase isoenzymes in serum, Clin. Chem., 34 (1988) 1885-1890.
17. Bergmeyer, H.U. and Bernt, E., Lactate dehydrogenase. In: H.U. Bergmeyer (Ed.), Methods of Enzymatic Analysis, 2nd edition, Verlag Chemie, Weinheim, 1974, pp. 574-579.
18. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., Protein measurement with the folin phenol reagent, J. Biol. Chem., 193 (1951) 265-275.
19. Pesce, A., Fondy, T.P., Stolzenbach, F., Castillo. F. and Kaplan, N.O., The comparative enzymology of lactic dehydrogenases, J. Biol. Chem., 242 (1967) 2151-2167.