Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine γ-synthase from E. coli

FEBS Letters

Volumn 414, Issue 3, 1997, Pages 492-496

  Wahl, Markus C ^a   Huber, Robert ^a   Prade, Lars ^a   Marinkovic, Snezan ^a   Messerschmidt, Albrecht ^a   Clausen, Tim ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Cystathionine synthase; Escherichia coli; Methionine biosynthesis; Protein crystallization; Pyridoxal 5' phosphate; X ray crystallography

Indexed keywords

CYSTATHIONE SYNTHETASE; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; CRYSTALLIZATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; EXTRACTION; GENE OVEREXPRESSION; MOLECULAR CLONING; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; X RAY DIFFRACTION;

CARBON-OXYGEN LYASES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; RECOMBINANT PROTEINS; ROTATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030768603     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(97)01057-0     Document Type: Article

References (26)

1. Alexander F.W., Sandmeier E., Mehta P.K., Christen P. Eur. J. Biochem. 219:1994;953-960.
2. Belfaiza J., Parsot C., Martel A., Bouthier de la Tour C., Margerita D., Cohen G.N., Saint-Girons I. Proc. Natl. Acad. Sci. USA. 83:1986;867-871.
3. Brzovic P., Litzenberger Holbrook E., Greene R.C., Dunn M.F. Biochemistry. 29:1990;442-451.
4. Guggenheim S. Methods Enzymol. 17:1971;439-442.
5. Flavin, M. (1975) in: Metabolic pathways (Greenberg, D.M., Ed.) vol. 7, pp. 457-503, Academic Press, New York.
6. Clausen T., Huber R., Laber B., Pohlenz H.-D., Messerschmidt A. J. Mol. Biol. 262:1996;202-224.
7. Guggenheim S., Flavin M. J. Biol. Chem. 244:1969;3722-3727.
8. Guggenheim S., Flavin M. J. Biol. Chem. 244:1969;6217-6227.
9. Johnston M., Jankowski D., Marcotte P., Tanaka H., Esaki N., Soda K., Walsh C. Biochemistry. 18:1979;4691-4701.
10. Datko A.H., Mudd S.H. Plant Physiol. 69:1982;1070-1076.
11. Thompson G.A., Datko A.H., Mudd S.H. Plant Physiol. 70:1982;1347-1352.
12. Kreft B.D., Townsend A., Pohlenz H.D., Laber B. Plant Physiol. 104:1994;1215-1220.
13. Zakin M.M., Greene R.C., Dautry-Varsat A., Duchange N., Ferrara P., Py M.-C., Margarita D., Cohen G.N. Mol. Gen. Genet. 187:1982;101-106.
14. Saiki R.K., Scharf S., Faloona F., Mullis K.B., Horn G.T., Erlich H.A., Arnheim N. Science. 230:1985;1350-1354.
15. Saiki R.K., Gelfand D.H., Stoffel S., Scharf S.J., Higuchi R., Horn G.T., Mullis K.B., Erlich H.A. Science. 239:1988;487-491.
16. Smith M., Jessee J., Landers T., Jordan J. Focus. 12:1991;38-40.
17. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual (Nolan, C., Ed.) Vol. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
18. Kaplan M., Flavin M. J. Biol. Chem. 241:1966;5781-5789.
19. Leslie, A.G.W. (1990) MOSFLM program. Abstract of the Crystallographic Computing School, pp. 27-38, Bischenberg.
20. Collaborative Computational Project Number 4 (1994) Acta Crystallogr. D50, 760-763.
21. Tong L., Rossman M.G. Acta Crystallogr. A46:1990;783-792.
22. Chong S., Yang S., Paulus H., Brenner J., Perler F.B., Xu M.-Q. J. Biol. Chem. 271:1996;22159-22168.
23. Litzenberger Holbrook E., Greene R.C., Heilig Krueger J. Biochemistry. 29:1990;435-442.
24. Johnston M., Marcotte P., Donovan J., Walsh C. Biochemistry. 18:1979;1729-1738.
25. Davis L., Metzler D.E. Enzymes. 7:1972;33-74.
26. Walsh, C.T. (1979) Enzymatic Reaction Mechanisms, pp. 777-827, Freeman, San Francisco, CA.