Computer analysis of corneal innervation density using a novel double stain in rat corneal whole mounts

Journal of Anatomy

Volumn 191, Issue 2, 1997, Pages 191-199

  Jacot, Jorge L ^a   Glover, Joel P ^a   Robison Jr , W Gerald ^a  

^a NATIONAL EYE INSTITUTE   (United States)

Author keywords

Acetylcholinesterase; Corneal nerves; Gold chloride; Morphometry

Indexed keywords

ACETYLCHOLINESTERASE; BUFFER; GOLD CHLORIDE; PARAFORMALDEHYDE; SODIUM DIHYDROGEN PHOSPHATE;

ANIMAL TISSUE; ARTICLE; CELL DENSITY; COMPUTER ANALYSIS; CORNEA; DESCEMET MEMBRANE; INNERVATION; MORPHOMETRICS; NONHUMAN; PHOTOGRAPHY; PRIORITY JOURNAL; RAT; SENSORY NERVE; STAINING;

ACETYLCHOLINESTERASE; ANIMALS; COLORING AGENTS; CORNEA; ENDOTHELIUM, CORNEAL; FEMALE; GOLD COMPOUNDS; IMAGE PROCESSING, COMPUTER-ASSISTED; MALE; RATS; RATS, SPRAGUE-DAWLEY;

ANIMALIA;

EID: 0030767680     PISSN: 00218782     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0021878297002331     Document Type: Article

References (19)

1. BEUERMAN RW, ROZSA AJ (1984) Collateral sprouts are replaced by regenerating neurites in the wounded corneal epithelium. Neuroscience Letters 44, 99-104.
2. CHAN KY, JARVELAINEN M, CHANG JH, EDENFIELD MJ (1990) A cryodamage model for studying corneal nerve regeneration. Investigative Ophthalmology and Visual Science 31, 2008-2021.
3. CHAN-LING T (1989) Sensitivity and neural organization of the cat cornea. Investigative Ophthalmology and Visual Science 30, 1075-1082.
4. ISHIDA N, DEL CERRO M, RAO GN, MATHE M, AQUAVELLA JV (1984a) Corneal stromal innervation: a quantitative analysis of distribution. Ophthalmic Research 16, 139-144.
5. ISHIDA N, RAO GN, DEL CERRO M, AQUAVELLA JV (1984b) Corneal nerve alterations in diabetes mellitus. Archives of Ophthalmology 102, 1380-1384.
6. JACOT JL, GLOVER JP, ROBISON, JR, WG (1995) Improved gold chloride procedure for nerve staining in whole mounts of rat corneas. Biotechnic and Histochemistry 70, 277-284.
7. KARNOVSKY MJ, ROOTS L (1964) A 'direct-coloring' thiocholine method for cholinesterases. Journal of Histochemistry and Cytochemistry 12, 219-221.
8. KOELLE GB (1951) The elimination of enzymatic diffusion artifacts in the histological localization of cholinesterases and a survey of their cellular distributions. Journal of Pharmacology and Experimental Therapeutics 103, 153-171.
9. MARGREET DE LEEUW A, CHAN KY (1989) Corneal nerve regeneration. Investigative Ophthalmology and Visual Science 30, 1980-1990.
10. OGILVY CS, BORGES LF (1990) Changes in corneal innervation during postnatal development in normal rats and in rats treated at birth with capsaicin. Investigative Ophthalmology and Visual Science 31, 1810-1815.
11. OGILVY CS, SILVERBERG KR, BORGES LF (1991) Sprouting of corneal sensory fibers in rats treated at birth with capsaicin. Investigative Ophthalmology and Visual Science 32, 112-121.
12. ROBERTSON DM, WINKELMANN RK (1970) A whole-mount cholinesterase technique for demonstrating corneal nerves: observations in the albino rabbit. Investigative Ophthalmology 9, 710-715.
13. ROSZA AJ, BEUERMAN RW (1982) Density and organization of free nerve endings in the corneal epithelium of the rabbit. Pain 14, 105-120.
14. SCHIMMELPFENNIG B (1982) Nerve structure in human central corneal epithelium. Graefe's Archives of Clinical and Experimental Ophthalmology 218, 14-20.
15. SILVERBERG KR, OGILVY CS, BORGES LF (1989) A modified gold chloride technique for optimal impregnation of nerves within corneal whole mounts and dura of the albino rat. Journal of Histochemistry and Cytochemistry 37, 269-271.
16. TERVO T (1976) Histochemical demonstration of cholinesterase activity in the cornea of the rat and the effect of various denervations on the corneal nerves. Histochemistry 47, 133-143.
17. TERVO T, PALKAMA A (1978) Innervation of the rabbit cornea: a histochemical and electron-microscopic study. Acta Anatomica 102, 164-175.
18. TRABUCCHI G, BRABCATO R, VERDI M, CARONES F, SALA C (1994) Corneal nerve damage and regeneration after excimer laser photokeratectomy in rabbit eyes. Investigative Ophthalmology and Visual Science 35, 229-235.
19. WEDDELL G, ZANDER E (1950) A critical evaluation of methods used to demonstrate tissue neural elements, illustrated by reference to the cornea. Journal of Anatomy 84, 168-195.