Ectoine functions as an osmoprotectant in Bacillus subtilis and is accumulated via the ABC-transport system OpuC

FEMS Microbiology Letters

Volumn 154, Issue 2, 1997, Pages 325-330

  Jebbar, Mohamed ^a,b   Von Blohn, Carsten ^a,b   Bremer, Erhard ^a,b  

^a MAX PLANCK INSTITUTE FOR TERRESTRIAL MICROBIOLOGY   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

ABC transporter; Bacillus subtilis; Ectoine; Glycine betaine; Osmoprotectant

Indexed keywords

ABC TRANSPORTER; AMINO ACID;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; CONTROLLED STUDY; ENVIRONMENT; NONHUMAN; OSMOLALITY; OSMOLARITY; PRIORITY JOURNAL; SOIL; SOIL MICROFLORA;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); BACTERIUM SUBTILIS; OPUA;

EID: 0030766369     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00349-2     Document Type: Article

References (18)

1. Whatmore, A.M., Chudek, J.A. and Reed, R.H. (1990) The effects of osmotic upshock on the intracellular solute pools of Bacillus subtilis. J. Gen. Microbiol. 136, 2527-2535.
2. Boch, J., Kempf, B. and Bremer, E. (1994) Osmoregulation in Bacillus subtilis: synthesis of the osmoprotectant glycine betaine from exogenously provided choline. J. Bacteriol. 176, 5364-5371.
3. Galinski, E.A. and Trüper, H.G. (1994) Microbial behaviour in salt-stressed ecosystems. FEMS Microbiol. Rev. 15, 95-108.
4. Lucht, J.M. and Bremer, E. (1994) Adaptation of Escherichia coli to high osmolarity environments: osmoregulation of the high-affinity glycine betaine transport system ProU. FEMS Microbiol. Rev. 14, 3-20.
5. von Blohn, C., Kempf, B., Kappes, R.M. and Bremer, E. (1997) Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B. Mol. Microbiol. 25, (in press).
6. + in turgor regulation. J. Gen. Microbiol. 136, 2521-2526.
7. Boch, J., Kempf, B., Schmid, R. and Bremer, E. (1996) Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes. J. Bacteriol. 178, 5121-5129.
8. Kappes, R.M., Kempf, B. and Bremer, E. (1996) Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: characterization of OpuD. J. Bacteriol. 178, 5071-5079.
9. Kempf, B. and Bremer, E. (1995) OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis. J. Biol. Chem. 270, 16701-16713.
10. Lin, Y. and Hansen, J.N. (1995) Characterization of a chimeric proU operon in a subtilin-producing mutant of Bacillus subtilis 168. J. Bacteriol. 177, 6874-6880.
11. Higgins, C.F. (1992) ABC transporters: from microorganisms to man. Annu. Rev. Cell Biol. 8, 67-113.
12. Talibart, R., Jebbar, M., Gouesbet, G., Himdi-Kabbab, S., Wróblewski, H., Blanco, C. and Bernard, T. (1994) Osmoadaptation in Rhizobia: ectoine-induced salt tolerance. J. Bacteriol. 176, 5210-5217.
13. Louis, P. and Galinski, E.A. (1997) Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli. Microbiology 143, 1141-1149.
14. Bernard, T., Jebbar, M., Rassouli, Y., Himdi-Kabbab, S., Hamelin, J. and Blanco, C. (1993) Ectoine accumulation and osmotic regulation in Brevibacterium linens. J. Gen. Microbiol. 139, 129-136.
15. Malin, G. and Lapidot, A. (1996) Induction of synthesis of tetrahydropyrimidine derivatives in Streptomyces strains and their effect on Escherichia coli in response to osmotic and heat stress. J. Bacteriol. 178, 385-395.
16. Severin, J., Wohlfarth, A. and Galinski, E.A. (1992) The predominant role of recently discovered tetrahydropyrimidines for the osmoadaptation of halophilic eubacteria. J. Gen. Microbiol. 138, 1629-1638.
17. Gouesbet, G., Trautwetter, A., Bonnassie, S., Wu, L.F. and Blanco, C. (1996) Characterization of the Erwinia chrysanthemi osmoprotectant transporter gene ousA. J. Bacteriol. 178, 447-455.
18. Jebbar, M., Talibart, R., Gloux, K., Bernard, T. and Blanco, C. (1992) Osmoprotection of Escherichia coli by ectoine: uptake and accumulation characteristics. J. Bacteriol. 174, 5027-5035.