Preanalytical and analytical pitfalls in molecular biology techniques

Journal of Clinical Ligand Assay

Volumn 20, Issue 2, 1997, Pages 200-205

  Narayanan, Sheshadri ^a  

^a METROPOLITAN HOSPITAL CENTER   (United States)

Author keywords

Analytical pitfalls; Contamination control; Molecular biology techniques; Preanalytical pitfalls

Indexed keywords

ANTICOAGULANT AGENT; DETERGENT; DNA; RIBONUCLEASE; RNA;

ANALYTICAL ERROR; CENTRIFUGATION; CONTAMINATION; DNA PROBE; LABORATORY TEST; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; REVIEW; TECHNIQUE; TEMPERATURE;

EID: 0030766028     PISSN: 10811672     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (27)

1. Narayanan S: Concepts, principles and applications of selected molecular biology techniques in Clinical Biochemistry. Adv Clin Chem 1996;32:1-38.
2. Winn-Deen ES: Automation of molecular genetic methods - Part 2: DNA amplification techniques. J Clin Ligand Assay 1996;19:21-26.
3. Narayanan S: Overview of principles and current uses of DNA probes in clinical and laboratory medicine. Ann Clin Lab Sci 1992;22:353-376.
4. Rolfs A, Shuller I, Finckh U, et al: PCR: Clinical Diagnostics and Research. New York, New York, Springer-Verlag, 1992, pp 51-60.
5. Buffone GJ, Darlington GJ: Isolation of DNA from biological specimens without extraction with phenol. Clin Chem 1985;30:164-165.
6. Guder WG, Narayanan S, Wisser H, et al: Samples: From the Patient to the Laboratory. The Impact of Preanalytical Variables on the Quality of Laboratory Results. Darmstadt, Germany, Git-Verlag, 1996 pp 63-65.
7. Holodniy M, Kim S, Katzenstein D, et al: Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol 1991;29:676-679.
8. Todd DM, Buccini FJ: Apparent heparin interference with restriction enzyme digestion of genomic DNA. Clin Chem 1993;39:362-363.
9. Coad JE, Lander TA, Litz CE: Inhibition of restriction endonucleases by common clinical anticoagulants. Anal Biochem 1992;205:368-369.
10. Beutler E, Gelbart T, Kuhl W: Interference of heparin with the polymerase chain reaction. Bio Techniques 1990;9:166.
11. Kilpatrick DC: Simplified preparation from anticoagulated blood for HLA-DR genomic typing. Tissue Antigens 1993;41:219-220.
12. Boyum A: Separation of lymphocytes from blood and bone marrow. Scand J Clin Lab Invest 1968;21(Suppl 97):77-106.
13. Muirhead KA, Wallace PK, Schmitt TC, et al: Methodological considerations for implementation of lymphocyte subset analysis in a clinical reference laboratory. Ann NY Acad Sci 1986;468:113-127.
14. Mole L, Margolis D, Carroll R, et al: Stabilities of quantitative plasma culture for human immunodeficiency virus, RNA, and p24 antigen from samples collected in VACUTAINER CPT and standard VACUTAINER Brand Tubes. J Clin Microbiol 1994;32:2212-2215.
15. Klebanoff SJ, Coombs RW: Viricidal effect of polymorphonuclear leukocytes on human immunodeficiency virus-1. Role of myeloperoxidase system. J Clin Invest 1992;89:2014-2017.
16. Saag MS, Holodniy M, Kuritzkes DR, et al: HIV viral load markers in clinical practice. Nature Med 1996;2:625-629.
17. Greer CE, Peterson SL, Kiviat NB, et al: PCR amplification from paraffin-embedded tissues: Effects of fixative and fixation time. Am J Clin Pathol 1991;95:117-124.
18. Wright DK, Manos MM: Sample preparation from paraffin-embedded tissues. In: Innis MA, Gelfand DH, Sninsky JJ, et al (eds) PCR Protocols: A Guide to Methods and Applications. San Diego, CA, Academic Press, Inc., 1990, pp 153-158.
19. Kiechle FL, Chambers LM, Cox RS, et al (eds): Patient preparation and specimen handling. Fascicle VII: Reference Guide to Diagnostic Molecular Pathology and Flow Cytometry. Northfield, IL, College of American Pathologists, 1996.
20. Bej AK, Mahbubani MH, Atlas RM: Amplification of nucleic acids by polymerase chain reaction (PCR) and other methods and their applications. Crit Rev Biochem Mol Biol 1991;26:301-334.
21. Sestini R, Orlando C, Zentilin L, et al: Measuring c-erb B-2 oncogene amplification in fresh and paraffin-embedded tumors by competitive polymerase chain reaction. Clin Chem 1994;40:630-636.
22. Houseman DE: Clinical implications of basic research. DNA on trial - The molecular basis of DNA fingerprinting. N Engl J Med 1995;332:534-535.
23. Wallace DC: Disease of the mitochondrial DNA. Annu Rev Biochem 1992;61:1175-1212.
24. Shoffner JM, Wallace DC. Oxidative phosphorylation diseases and mitochondrial DNA mutations: diagnosis and treatment. Annu Rev Nutr 1994;14:535-568.
25. DeSalle R, Williams AK, George M: Isolation and characterization of animal mitochondrial DNA. In: Zimmer EA, White TJ, Cann RL, et al (eds) Methods Enzymol. San Diego, CA, Academic Press, Inc., 1993, vol. 224, pp 176-204.
26. Inazuka M, Tahira T, Hayashi K: One-tube post-PCR fluorescent labeling of DNA fragments. Genome Res 1996;6:551-557.
27. Longo MC, Berninger MS, Hartley JL: Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene 1990;93:125-128.