Enzymatic fucosylations of non-natural acceptors with non-natural donor-sugars

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 18, 1997, Pages 2431-2434

  Baisch, Gabi ^a   Öhrlein, Reinhold ^a   Katopodis, Andreas ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FUCOSYLTRANSFERASE; SIALIC ACID DERIVATIVE;

ARTICLE; ENZYME ACTIVITY; FUCOSYLATION; IN VITRO STUDY; REACTION ANALYSIS; STEREOCHEMISTRY; STEREOSPECIFICITY;

EID: 0030765856     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00447-2     Document Type: Article

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18. 2-solution and 470 μ1 (250 mM) sodium cacodylate-buffer (pH = 6.52). The slightly turbid mixture is then incubated with 30 U (2 μ1) calf intestine alkaline phosphatase (Boehringer no. 108146, 7500 U/498 μ1) and 0.8 U (40 μ1) of a Fuc-T VI solution at 37° C for 48 h. The mixture is then passed over a short C-18 reversed phase column, washed with water and eluted with methanol and then lyophilized from water. The residue is finally purified on silica gel (eluent: dichloromethane - methanol - water mixtures). The product-containing fractions are combined, evaporated to dryness and lyophilized from dioxane - water to give 10.0 mg (81%) of the tetrasaccharide 3 (entry 7) as a white, fluffy powder.
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