Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function

Molecular and Cellular Biochemistry

Volumn 174, Issue 1-2, 1997, Pages 55-60

  Rasmussen, Hans N ^a   Rasmussen, Ulla F ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Cytochrome c conservation; Respiration measurements; Skeletal muscle mitochondria; Small scale preparation

Indexed keywords

CYTOCHROME C; MITOCHONDRIAL ENZYME;

ANIMAL TISSUE; ARTICLE; HEART MITOCHONDRION; LOW TEMPERATURE; MITOCHONDRIAL MEMBRANE; MITOCHONDRIAL RESPIRATION; MUSCLE MITOCHONDRION; NONHUMAN; PIGEON; SKELETAL MUSCLE; SPECTROSCOPY; SWINE;

ADENOSINE TRIPHOSPHATE; ANIMALS; COLUMBIDAE; CYTOCHROME C GROUP; ELECTRON TRANSPORT; MITOCHONDRIA, MUSCLE; MUSCLE, SKELETAL;

ANIMALIA; COLUMBA; SUS SCROFA;

EID: 0030765108     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006851705996     Document Type: Article

References (14)

1. Chappell JB, Perry SV: Biochemical and osmotic properties of skeletal muscle mitochondria. Nature 173: 1094-1095, 1954
2. Mickelson JR, Greaser ML, Marsh BB: Purification of skeletal-muscle mitochondria by density-gradient centrifugation with percoll. Anal Biochem 109: 255-260, 1980
3. De Duve C, Berthet J: Reproducibility of differential centrifugation experiments in tissue fractionation. Nature 172: 1142, 1953
4. Schaffner W, Weissmann C: A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem 56: 502-514, 1973
5. Shepherd D, Garland PB: Citrate synthase from rat liver. Meth Enzymol XIII: 11-16, 1969
6. Rasmussen HN, Rasmussen UF: Respiration measurements in small scale. Anal Biochem 208: 244-248, 1993
7. Hartree EF: Haematin compounds. In: K Paech, MW Tracey (eds). Modern Methods of Plant Analysis vol. 4. Springer, Berlin, 1955, pp 208-210
8. van Gelder BF: Optical properties of cytochromes from beef heart mitochondria, submitochondrial vesicles, and derived preparations. Meth Enzymol LIII: 125-128, 1978
9. Rasmussen UF, Rasmussen HN, Jørgensen BM: Three functionally different cytochrome b redox centres in pigeon heart mitochondria. Biochem J 201: 311-320, 1982
10. Nedergaard J, Cannon B: Overview - preparation and properties of mitochondria from different sources. Meth Enzymol LV: 3-28, 1979
11. Hafner RD, Brown GC, Brand MD: Analysis of the control of respiration rate, phosphorylation rate, proton leak rate and protonmotive force in isolated mitochondria using the 'top-down' approach of metabolic control theory. Eur J Biochem 188: 313-319, 1990
12. Rasmussen UF, Rasmussen HN, Andersen AJ, Fogd Jørgensen P, Quistorff B: Characterization of mitochondria from pig muscle: higher activity of exo-NADH oxidase in animals suffering from malignant hyperthermia. Biochem J 315: 659-663, 1996
13. Li X-Z, Kumasa S, Isogai M, Mori M: Histochemical differences in flight and leg muscles of the pigeon. Acta Histochem Cytochem 21: 585-592, 1988
14. Essén B, Lindholm A: Muscle fiber types and glycogen depletion pattern in halothane sensitive pigs. In: T Frøystein, E Slinde, N Standal (eds). Porcine Stress and Meat Quality. Agr Food Res Soc. Norway, 1981, pp 42-52