Application of automatic thresholding in image analysis scoring of cells in human solid tumors labeled for proliferation markers

Cytometry

Volumn 29, Issue 2, 1997, Pages 128-135

  Weaver, Jean R ^a   Au, Jessie L S ^a,b,c  

^a OHIO STATE UNIVERSITY   (United States)

^b OHIO STATE UNIVERSITY   (United States)

^c NONE   (United States)

Author keywords

BrdUrd; HSI; Image analysis; Image cytometry; Immunohistochemistry; PCNA; Quality assurance; Segmentation; Threshold

Indexed keywords

BROXURIDINE; CYCLINE;

ARTICLE; AUTOMATION; BLADDER CANCER; CANCER GROWTH; CELL PROLIFERATION; CYTOMETRY; GOOD LABORATORY PRACTICE; HEAD AND NECK CANCER; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL;

ANALYSIS OF VARIANCE; BROMODEOXYURIDINE; COLOR PERCEPTION; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROTOMY; NEOPLASMS; PARAFFIN EMBEDDING; PROLIFERATING CELL NUCLEAR ANTIGEN; REPRODUCIBILITY OF RESULTS; SENSORY THRESHOLDS; STAINING AND LABELING;

EID: 0030763734     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19971001)29:2<128::AID-CYTO5>3.0.CO;2-9     Document Type: Article

References (18)

1. Geerts H, Nuydens R, Nuyens R, Ver Donck L: The effect of flunarizine on intracellular calcium in isolated rat cardiomyocytes. Cardiovasc Res 23:797-806, 1989.
2. Humm JL, Macklis RM, Yang Y, Bump K, Chin LM: Image analysis for the study of radionuclide distribution in tissue sections. J Nucl Med 35:1217-1225, 1994.
3. Ito M, Ohki M, Hayashi K, Yamada M, Uetani M, Nakamura T: Trabecular texture analysis of CT images in the relationship with spinal fracture Radiology 194:55-59, 1995.
4. Karlsson MG, Davidsson A, Hellquist HB: Quantitative computerized image analysis of immunostained lymphocytes. A methodological approach. Pathol Res Pract 190:799-807, 1994.
5. Knuechel R, Burgau M, Rueschoff J, Hofstaedter F: Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: A quantitative assessment using a true color image analysis system. Virchow Arch B 64:137-144, 1993.
6. Lamaziere JM, Lavallee J, Zunino C, Larrue J: Semiquantitative study of the distribution of two cellular antigens by computer-directed color analysis. Lab Invest 68:248-252, 1993.
7. Makkus AC, van't Hof-Grootenboer AE, Pahlplatz MM, de Wilde PC, Gemmink AJ, Cuypers VM, Vooijs GP: Practical aspects of fixatives in high resolution nuclear image analysis. Cytometry 15:302-310, 1994.
8. Manukata S, Hendricks JB: Effect of fixation time and microwave oven heating time on retrieval of the Ki-67 antigen from paraffin-embedded tissue. J Histochem and Cytochem 41:1241-1246, 1993.
9. Mize RR, Holdefer RN, Nabors LB: Quantitative immunocytochemistry using an image analyzer. I. Hardware evaluation, image processing, and data analysis. J Neurosci Methods 26:1-23, 1988.
10. Montironi R, Galluzzi CM, Diamanti L, Giannulis I, Pisani E, Scarpelli M: Prostatic intra-epithelial neoplasia: Expression and location of proliferating cell nuclear antigen in epithelial, endothelial and stromal nuclei. Virchow Arch A Pathol Anat Histopathol 422:185-192, 1993
11. Otsu N: A threshold selection method from gray-level histograms. IEEE Trans Syst Man Cybern SMC-6:62-66, 1979.
12. Sahoo PK, Soltani S, Wong AKC, Chen YC: A survey of thresholding techniques. Comput Vision Graphics Image Process 41:233-260, 1988.
13. Sallinen P, Haapasalo H, Kerttula T, Rantala I, Kalimo H, Collan Y, Isola J, Helin H: Sources of variation in the assessment of cell proliferation using proliferating cell nuclear antigen immunohistochemistry. Anal Quant Cytol Histol 16:261-268, 1994.
14. Schipper NW, Smeulders AW, Baak JP: Evaluation of automated estimation of epithelial volume and its prognostic value in ovarian tumors. Lab Invest 61:228-234, 1989.
15. Siitonen SM, Isola JJ, Rantala IS, Helin HJ: Intratumor variation in cell proliferation in breast carcinoma as determined by antiproliferating cell nuclear antigen monoclonal antibody and automated image analysis. Am J Clin Pathol 99:226-231, 1993.
16. Verrier J, Waite I, Linney A, Sutton D, Galgut P: A microcomputer system for the analysis of dental radiographs. Br Dent J 167:135-139, 1989.
17. Weaver JR, Au JLS: Comparative image and visual analysis of proliferation markers in human solid tumors. Cytometry 27:189-199, 1997.
18. Wu Y, Lemkin PF, Upton K: A fast spot segmentation algorithm for two-dimensional gel electrophoresis analysis. Electrophoresis 14:1351-1356, 1993.