Dysregulation of carcinoembryonic antigen group members CGM2, CD66a (biliary glycoprotein), and nonspecific cross-reacting antigen in colorectal carcinomas: Comparative analysis by Northern blot and in situ hybridization

American Journal of Pathology

Volumn 151, Issue 2, 1997, Pages 521-530

  Nollau, Peter ^a   Prall, Friedrich ^a   Helmchen, Udo ^a   Wagener, Christoph ^a   Neumaier, Michael ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARCINOEMBRYONIC ANTIGEN; CROSS REACTING ANTIGEN; GLYCOPROTEIN; GLYCOPROTEIN CD 66 A; KI 67 ANTIGEN; UNCLASSIFIED DRUG;

ARTICLE; AUTORADIOGRAPHY; CLINICAL ARTICLE; COLORECTAL CARCINOMA; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IN SITU HYBRIDIZATION; NORTHERN BLOTTING; PRIORITY JOURNAL;

EID: 0030762659     PISSN: 00029440     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Hammarström S, Khan WN, Teglund S, Hammarström ML, Ramos T, Baranov W, Yeung MM, Frängsmyr L: The carcinoembryonic antigen family. Structures of Antigens, vol 2. Edited by MHV VanRegenmortel. Boca Raton, FL, CRC Press, 1993, pp 341-376
2. Brandriff BF, Gordon LA, Olsen AS, Mohrenweiser HW, Fertitta A, Carrano AV, Trask BJ: Order and genomic distances among members of carcinombyonic antigen (CEA) gene family determined by fluorescence in situ hybridization. Genomics 1992, 12:773-779
3. Barnett TR, Drake L, Pickle W: Human biliary glycoprotein gene: characterization of a family of novel alternatively spliced RNAs and their expressed proteins. Mol Cell Biol 1993, 13:1273-1282
4. Hinoda Y, Neumaier M, Hefta SA, Drzeniek Z, Wagener C, Shively L, Hefta LJF, Shively JE, Paxton RJ: Molecular cloning of a cDNA coding biliary glycoprotein I: primary structure of a glycoprotein immunologically crossreactive with carcinoembryonic antigen. Proc Natl Acad Sci USA 1988, 85:6959-6963
5. Barnett TR, Kretschmer A, Austen DA, Goebel SJ, Hart JT, Elting JJ, Kamarck ME: Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family. J Cell Biol 1989, 108:267-276
6. Thompson JA, Grunert F, Zimmermann W: Carcinoembryonic gene family: molecular biology and clinical perspectives. J Clin Lab Anal 1991, 5:344-366
7. Blikstad I, Wikström T, Aurivillius M, Öbrink B: C-CAM (Cell-CAM 105) is a calmodulin binding protein. FEBS Lett 1992, 302:26-30
8. Skubitz KM, Ducker TP, Skubitz APN, Goueli SA: Antiserum to carcinoembryonic antigen recognizes a phosphotyrosine-containing protein in human colon cancer cell lines. FEBS Lett 1993, 318:200-204
9. c-src witn biliary glycoprotein (CD66a), an adhesion molecule of the carcinoembryonic antigen family downregulated in colorectal carcinomas. Oncogene 1995, 11:1649-1655
10. Odin P, Öbrink B: The cell-surface expression of the cell adhesion molecule Cell-CAM 105 in rat fetal tissues and regenerating liver. Exp Cell Res 1988, 179:89-103
11. Tingström A, Öbrink B: Distribution and dynamics of cell surface-associated Cell-CAM 105 in cultured rat hepatocytes. Exp Cell Res 1989, 185:132-142
12. Hansson M, Blikstad I, Öbrink B: Cell-surface location and molecular properties of Cell-CAM 105 in intestinal epithelial cells. Exp Cell Res 1989, 181:63-74
13. Neumaier M, Paululat S, Chan A, Matthaes P, Wagener C: Biliary glycoprotein, a potential human cell adhesion molecule, is downregulated in colorectal carcinomas. Proc Natl Acad Sci USA 1993, 90:10744-10748
14. Thompson J, Zimmermann W, Nollau P, Neumaier M, Weber-Arden J, Schrewe H, Craig I, Willcocks T: CGM2, a member of the carcinoem bryonic antigen gene family, is down-regulated in colorectal carcinomas. J Biol Chem 1994, 269:32924-32931
15. Fearon ER, Vogelstein B: A genetic model for colorectal tumorigenesis. Cell 1990, 61:759-767
16. Cattoretti G, Becker MHG, Dey G, Duchrow M, Schlüter C, Galle J, Gerdes J: Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MiB1 and MiB3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992, 168:357-363
17. Frängsmyr L, Baranov V, Prall F, Yeung M-W, Wagener C, Hammarström S: Cell- and region-specific expression of biliary glycoprotein and its messenger RNA in normal human colonic mucosa. Cancer Res 1995, 55:2963-2967
18. Lipkins M: Proliferation and differentiation of normal and neoplastic cells in the colon of man. Cancer 1971, 28:38-40
19. Wiebecke B, Brandts A, Eder M: Epithelial proliferation and morphogenesis of hyperplastic adenomatous and villous polyps of the human colon. Virchows Arch A Pathol Anat Histol 1974, 364:35-49
20. Kaye GI, Fenoglio CM, Pascal RR, Lane N: Comparative electron microscopic features of normal, hyperplastic, and adenomatous human colonic epithelium. Gastroenterology 1973, 64:926-945
21. Mughal S, Filipe MI: Ultrastructural study of the normal mucosa and the adenoma-cancer sequence in the development of familial polyposis coli syndrome. J Natl Cancer Inst 1978, 60:753-768
22. Mickey WF, Seiler MW: Ultrastructural markers of colonic adenocarcinomas. Cancer 1981, 47:140-145
23. Mughal S, Filipe MI, Jass JR: A comparative ultrastructural study of hyperplastic and adenomatous polyps, incidental and in association with colorectal cancer. Cancer 1981, 48:2755-2755
24. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, ed 2. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, 1989
25. Leube RE, Bosch FX, Romano Y, Zimbelmann R, Höfler H, Franke WW: Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ. Differentiation 1989, 33:69-85
26. Thompson JA: Molecular cloning and expression of carcinoembryonic antigen gene family members. Tumor Biol 1995, 16:10-16
27. Wagener C, Csaszar H, Totovic V, Breuer H: A highly sensitive method for the demonstration of carcinoembryonic antigen in normal and neoplastic colonic tissue. Histochemistry 1981, 58:1-11
28. Wagener C, Müller-Wallraf R, Nissen S, Gröner J, Breuer H: Localization and concentration of carcinoembryonic antigen (CEA) in gastrointestinal tumours: correlation with CEA levels in plasma. J Natl Cancer Inst 1981, 57:539-547
29. Takahashi H, Okai Y, Paxton RJ, Hefta LFH, Shively JE: Differential regulation of carcinoembryonic antigen and biliary glycoprotein by γ-interferon. Cancer Res 1993, 53:1612-1619
30. Prall F, Nollau P, Neumaier M, Haubeck H-D, Drzeniek Z, Helmchen U, Löning T, Wagener C: CD66a (BGP), an adhesion molecule of the carcinoembryonic antigen family, is expressed in epithelium, endothelium, and myeloid cells in a wide range of normal human tissues. J Histochem Cytochem 1996, 44:35-41
31. Stoffel A, Neumaier M, Gaida FJ, Fenger U, Drzeniek Z, Haubeck HD, Wagener C: Monoclonal, anti-domain, and anti-peptides antibodies assign the molecular weight 160,000 antigen of the CD66 cluster to a mRNA encoded by the biliary glycoprotein (BGP) gene, a member of the carcinoembryonic antigen (CEA) gene family. J Immunol 1993, 150:4978-4984
32. Kunath T, Ordonez-Garcia C, Turbide C, Beauchemin N: Inhibition of colonic tumor cell growth by biliary glycoprotein. Oncogene 1995, 11:2375-2382
33. Neumaier M, Fenger U, Wagener C: Monoclonal antibodies for carcinoembryonic antigen (CEA) as a model system: identification of two novel CEA-related antigens in meconium and colorectal carcinoma tissues by Western blots and differential immunoaffinity chromatography. J Immunol 1985, 135:3604-3609
34. 3. Gastroenterology 1961, 41:122-125
35. Mukherjee TM, Williams AW: A comparative study of the ultrastructure of microvilli in the epithelium of small and large intestine of mice. J Cell Biol 1967, 34:447-461
36. Lorenzsson V, Trier JS: The fine structure of human rectal mucosa. Gastroenterology 1968, 55:88-101
37. Hsieh J-T, Luo W, Song W, Wang Y, Kleinerman DI, Van NT, Lin S-H: Tumor suppressive role of an androgen-regulated epithelial cell adhesion molecule (C-CAM) in prostate carcinoma cell revealed by sense and antisense approaches. Cancer Res 1995, 55:190-197