Alanyl-PNA oligomers: A new system for intercalation

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 13, 1997, Pages 1743-1746

  Diederichsen, Ulf ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; AMINOACRIDINE; DACTINOMYCIN; DAUNORUBICIN; DOUBLE STRANDED DNA; DYE; ETHIDIUM BROMIDE; OLIGOMER; PEPTIDE NUCLEIC ACID; POLYCYCLIC AROMATIC COMPOUND; PROFLAVINE;

ARTICLE; DNA STRUCTURE; DRUG DNA INTERACTION; DRUG STRUCTURE; NUCLEIC ACID STRUCTURE; OLIGOMERIZATION; STOICHIOMETRY;

EID: 0030762065     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00302-8     Document Type: Article

References (18)

1. Berman, H. M.; Young, P. R.; Annu. Rev. Biophys. Bioeng. 1981, 10, 87.
2. Saenger, W.; 'Principles of Nucleic Acid Structure' Springer Verlag New York 1984, 350.
3. 2+).
4. a) Diederichsen, U.; Angew. Chem. Int. Ed. Engl. 1996, 35, 445.
5. b) Diederichsen, U; Schmitt, H. W.; Tetrahedron Lett. 1996, 37, 475.
6. m, = 67°C (Hyrup, B.; Nielsen, P. E.; Bioorg. Med. Chem. 1996, 4, 5).
7. m, = 67°C (Hyrup, B.; Nielsen, P. E.; Bioorg. Med. Chem. 1996, 4, 5).
8. m, = 67°C (Hyrup, B.; Nielsen, P. E.; Bioorg. Med. Chem. 1996, 4, 5).
9. It is quite likely that the double strand regains stacking and compensates for the hole e.g. by helicalization around the glycine units.
10. 4, pH 7. After heating to 60°C the sample was kept at 0°C for 3 h. The melting curve was measured in 3h at a wavelength not interfering with the main absorption band of the intercalator (Figure 4).
11. The concentrations were estimated by UV absorption at 260 nm (PNA), 400 nm (9-aminoacridine) and 481 nm (ethidium bromide).
12. Quigley, G. J.; Wang, A. H.-J.; Ughetto, G.; van der Marel, G.; van Boom, J. H., Rich, A.; Proc. Natl. Acad. Sci. USA 1978, 75, 828.
13. Sobell, H. M.; Jain, S. C.; J. Mol. Biol. 1972, 68, 21.
14. Wittung, P.; Kim, S. K.; Buchardt, O.; Nielsen, P.; Nordèn, B.; Nucleic Acid Res. 1994, 22, 5371.
15. a) Fromherz, P.; Rieger, B.; J. Am. Chem. Soc. 1986, 108, 5361.
16. b) Hall, D. B.; Holmlin, R. E.; Barton, J. K.; Nature 1996, 382, 731.
17. c) Murphy, C. J.; Arkin, M.R.; Jenkins, Y.; Ghatlia, N. D.; Bossmann, S.H.; Turro, N.J.; Barton, J. K.; Science 1993, 262, 1025.
18. d) Beratan, D. N.; Priyadarsky, S.; Risser, S. M.; Chemistry & Biology 1997, 4, 3.