Postsynaptic glutamate transport at the climbing fiber-Purkinje cell synapse

Science

Volumn 277, Issue 5331, 1997, Pages 1515-1518

  Otis, Thomas S ^a   Kavanaugh, Michael P ^a   Jahr, Craig E ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1 (4 AMINOPHENYL) 4 METHYL 7,8 METHYLENEDIOXY 5H 2,3 BENZODIAZEPINE; 6 NITRO 7 SULFAMOYLBENZO[F]QUINOXALINE 2,3 DIONE; AMPA RECEPTOR; GLUTAMIC ACID; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; PHOSPHONIC ACID DERIVATIVE;

AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; CEREBELLUM; CLIMBING FIBER; MEMBRANE CURRENT; MEMBRANE ELECTROPHYSIOLOGY; NEUROTRANSMISSION; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PURKINJE CELL; XENOPUS;

AMINO ACID TRANSPORT SYSTEM X-AG; ANIMALS; ASPARTIC ACID; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; DICARBOXYLIC ACIDS; GLUTAMATE PLASMA MEMBRANE TRANSPORT PROTEINS; GLUTAMIC ACID; KINETICS; NERVE FIBERS; OOCYTES; PATCH-CLAMP TECHNIQUES; PURKINJE CELLS; PYRROLIDINES; RATS; RECEPTORS, AMPA; RECEPTORS, GLUTAMATE; SYMPORTERS; SYNAPSES; SYNAPTIC TRANSMISSION; XENOPUS;

EID: 0030761115     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5331.1515     Document Type: Article

References (39)

1. J. D. Rothstein et al., Neuron 13, 713 (1994); K. Yamada et al., Neuroreport 7, 2013 (1996); Y. Kanai, P. G. Bhide, M. DiFiglia, M. A. Hediger, ibid. 6, 2357 (1995).
2. J. D. Rothstein et al., Neuron 13, 713 (1994); K. Yamada et al., Neuroreport 7, 2013 (1996); Y. Kanai, P. G. Bhide, M. DiFiglia, M. A. Hediger, ibid. 6, 2357 (1995).
3. J. D. Rothstein et al., Neuron 13, 713 (1994); K. Yamada et al., Neuroreport 7, 2013 (1996); Y. Kanai, P. G. Bhide, M. DiFiglia, M. A. Hediger, ibid. 6, 2357 (1995).
4. M. Takahshi, M. Sarantis, D. Attwell, J. Physiol. 497, 523 (1996); Y. Kataoka and H. Ohmori, J. Neurophysiol. 76, 1870 (1996).
5. M. Takahshi, M. Sarantis, D. Attwell, J. Physiol. 497, 523 (1996); Y. Kataoka and H. Ohmori, J. Neurophysiol. 76, 1870 (1996).
6. G. B. Grant and J. E. Dowling, J. Neurosci. 15, 3852 (1995); S. A. Picaud, H. P. Larsson, G. B. Grant, H. Lecar, F. S. Werblin, J. Neurophysiol. 74, 1760 (1995); B. Billups, D. Rossi, D. Attwell, J. Neurosci. 16, 6722 (1996).
7. G. B. Grant and J. E. Dowling, J. Neurosci. 15, 3852 (1995); S. A. Picaud, H. P. Larsson, G. B. Grant, H. Lecar, F. S. Werblin, J. Neurophysiol. 74, 1760 (1995); B. Billups, D. Rossi, D. Attwell, J. Neurosci. 16, 6722 (1996).
8. G. B. Grant and J. E. Dowling, J. Neurosci. 15, 3852 (1995); S. A. Picaud, H. P. Larsson, G. B. Grant, H. Lecar, F. S. Werblin, J. Neurophysiol. 74, 1760 (1995); B. Billups, D. Rossi, D. Attwell, J. Neurosci. 16, 6722 (1996).
9. S. Eliasof and C. E. Jahr, Proc. Natl. Acad. Sci. U.S.A. 93, 4153 (1996).
10. W. A. Fairman, R. J. Vandenberg, J. L. Arriza, M. P. Kavanaugh, S. G. Amara, Nature 375, 599 (1995).
11. J. I. Wadiche, S. G. Amara, M. P. Kavanaugh, Neuron 15, 721 (1995).
12. J. L. Arriza, S. Eliasof, M. P. Kavanaugh, S. G. Amara, Proc. Natl. Acad. Sci. U.S.A. 94, 4155 (1997).
13. +-based solutions, respectively. Stimuli (10 to 100 V, 10 to 100 μs, 0.067 to 0.1 Hz) were delivered by means of a patch pipette filled with bath solution. Signals were filtered at 1 or 2 kHz (EPSCs) and digitized at 5 to 10 kHz. Membrane potential values have been corrected for junction potentials. Reported values are the mean ± SEM.
14. +-based solutions, respectively. Stimuli (10 to 100 V, 10 to 100 μs, 0.067 to 0.1 Hz) were delivered by means of a patch pipette filled with bath solution. Signals were filtered at 1 or 2 kHz (EPSCs) and digitized at 5 to 10 kHz. Membrane potential values have been corrected for junction potentials. Reported values are the mean ± SEM.
15. J. Ferkany and J. T. Coyle, Neurosci. Res. 16, 491 (1986).
16. J. L. Arriza et al., J. Neurosci. 14, 5559 (1994).
17. 2, 100 μM picrotoxin, 25 μM NBQX, 25 μM GYKl 52466, and 2.5 μM ±CPP (pH 7.4).
18. T. S. Otis, Y. C. Wu, L. O. Trussell, J. Neurosci. 16, 1634 (1996).
19. J. D. Clements, Trends Neurosci. 19, 163 (1996).
20. B. Barbour, B. U. Keller, I. Llano, A. Marty, Neuron 12, 1331 (1994); M. Takahashi, Y. Kovalchuk, D. Attwell, J. Neurosci. 15, 5693 (1995).
21. B. Barbour, B. U. Keller, I. Llano, A. Marty, Neuron 12, 1331 (1994); M. Takahashi, Y. Kovalchuk, D. Attwell, J. Neurosci. 15, 5693 (1995).
22. N. Zerangue and M. P. Kavanaugh, Nature 383, 634 (1996).
23. 2, and 5 mM Hepes (pH 7.5)].
24. 2 (n = 9). Mean EPSCs or STCs were integrated over 200 ms. Glutamate flux was estimated as the time integral of the STC divided by the mean charge/flux value for either EAAT3 or EAAT4 (16). These calculations assume the same proportion of anion current to glutamate flux throughout the STC as was measured at steady state in the oocyte experiments.
25. aEPSC (range: 270 to 746, n = 10).
26. N. Riveros, J. Fiedler, N. Lagos, C. Munoz, F. Orrego, Brain Res. 386, 405 (1986); D. Bruns and R. Jahn, Nature 377, 62 (1995).
27. N. Riveros, J. Fiedler, N. Lagos, C. Munoz, F. Orrego, Brain Res. 386, 405 (1986); D. Bruns and R. Jahn, Nature 377, 62 (1995).
28. S. Mennerick and C. F. Zorumski, Nature 368, 59 (1994).
29. T. Muller and H. Kettenman, Int. Rev. Neurobiol. 38, 341 (1995).
30. N. C. Danbolt, G. Pines, B. I. Kanner, Biochemistry 29, 6734 (1990); but see also E. A. Schwartz and M. Tachibana, J. Physiol. 426, 43 (1990).
31. N. C. Danbolt, G. Pines, B. I. Kanner, Biochemistry 29, 6734 (1990); but see also E. A. Schwartz and M. Tachibana, J. Physiol. 426, 43 (1990).
32. J. I. Wadiche, J. L. Arriza, S. G. Amara, M. P. Kavanaugh, Neuron 14, 1019 (1995).
33. D. Bruns, F. Engbert, H. D. Lux, ibid. 10, 559 (1993).
34. C.-M. Tang, M. Dichter, M. Morad, Science 243, 1474 (1989); D. J. A. Wyllie, S. F. Traynelis, S. G. Cull-Candy, J. Physiol. 463, 193 (1993); S. F. Traynelis, R. A. Silver, S. G. Cull-Candy, Neuron 11, 279 (1993).
35. C.-M. Tang, M. Dichter, M. Morad, Science 243, 1474 (1989); D. J. A. Wyllie, S. F. Traynelis, S. G. Cull-Candy, J. Physiol. 463, 193 (1993); S. F. Traynelis, R. A. Silver, S. G. Cull-Candy, Neuron 11, 279 (1993).
36. C.-M. Tang, M. Dichter, M. Morad, Science 243, 1474 (1989); D. J. A. Wyllie, S. F. Traynelis, S. G. Cull-Candy, J. Physiol. 463, 193 (1993); S. F. Traynelis, R. A. Silver, S. G. Cull-Candy, Neuron 11, 279 (1993).
37. G. Tong and C. E. Jahr, Neuron 13, 1195 (1994); J. S. Diamond and C. E. Jahr, J. Neurosci. 17, 4672 (1997).
38. G. Tong and C. E. Jahr, Neuron 13, 1195 (1994); J. S. Diamond and C. E. Jahr, J. Neurosci. 17, 4672 (1997).
39. We thank D. Bergles, J. Diamond, J. Dzubay, S. Eliasof, M. Jones, and J. Wadiche for helpful suggestions and comments on the manuscript; L. Klamo, S. Eliasof, J. Wadiche, and T. Zable for assistance with the oocyte experiments; and J. Diamond for the mEPSC analysis program. Supported by NS21419 (C.E.J.) and NS33270 (M.P.K.).