Reduction of connexin43 expression and dye-coupling during neuronal differentiation of human NTera2/clone D1 cells

Journal of Neuroscience Research

Volumn 49, Issue 1, 1997, Pages 19-31

  Bani Yaghoub, Mahmud ^a   Bechberger, John F ^a   Naus, Christian C G ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

Brain; Communication; Development; Embryonic; Gap junction; Retinoic acid

Indexed keywords

CONNEXIN 43;

ARTICLE; CELLULAR DISTRIBUTION; GROWTH REGULATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; NERVE CELL DIFFERENTIATION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS REGULATION;

CELL COMMUNICATION; CELL DIFFERENTIATION; CONNEXIN 43; FLUORESCEINS; FLUORESCENT DYES; GAP JUNCTIONS; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; LUNG NEOPLASMS; MALE; MICROSCOPY, FLUORESCENCE; NEOPLASM PROTEINS; NERVE TISSUE PROTEINS; NEURONS; RNA, MESSENGER; RNA, NEOPLASM; TERATOCARCINOMA; TRETINOIN; TUMOR CELLS, CULTURED;

EID: 0030759376     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19970701)49:1<19::AID-JNR3>3.0.CO;2-G     Document Type: Article

References (57)

1. Andrews PW, Damjanov I, Simon D. Banting GS, Carlin C, Dracopoli NC, Fogh J (1984): Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Lab Invest 50:147-162.
2. Bain G, Ray WJ, Yao M, Gottlieb DI (1994): From embryonal carcinoma cells to neurons: The P19 pathway. Bioessays 16:343-348.
3. Belliveau DJ, Kidder GM, Naus CCG (1991): Expression of gap junction genes during postnatal neural development, Dev Gen 12:308-317.
4. Belliveau DJ, Naus CCG (1995a): Expression of gap junctions in neural cells derived from P19 embryonal carcinoma cells. In Kanno Y, Kataoka K, Shiba Y, Shibata Y, Shimazu T (eds): "Intercellular Communication Through Gap Junctions: Progress in Cell Research Vol 4." Amsterdam: Elsevier. pp 251-255.
5. Belliveau DJ, Naus CCG (1995b): Cellular localization of gap junction mRNAs in developing rat brain. Dev Neurosci 17:81-96.
6. Bex V, Mercier T, Chaumontet C, Gaillard-Sanchez I, Flechon B, Mazet F, Traub O, Martel P (1995): Retinoic acid enhances connexin43 expression at the post-transcriptional level in rat liver epithelial cells. Cell Biochem Func 13:69-77.
7. Beyer EC, Paul DL, Goodenough DA (1987): Connexin43: A protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 105:2621-2629.
8. Cheung WMW, Chu AH, Leung MF, Ip NY (1995): Differentiation of embryonal carcinoma cells by neurotrophic factors and retinoic acid. Abst Soc Neurosci 21:1050.
9. Chiu FC, Rozental R, Bassallo C, Lyman WD, Spray DC (1994): Human fetal neurons in culture: Intercellular communication and voltage- and ligand-gated responses. J Neurosci Res 38:687-697.
10. Connors BW, Benardo LS, Prince DA (1983): Coupling between neurons of the developing rat neocortex. J Neurosci 3:773-782.
11. Dermietzel R, Spray DC (1993): Gap junctions in the brain: Where, what type, how many and why? Trends Neurosci 16:186-192.
12. Dermietzel R, Traub O, Hwang TK, Beyer E, Bennet VL, Spray DC (1989): Differential expression of three gap junction proteins in developmental and mature brain tissues. Proc Natl Acad Sci USA 86:10148-10152.
13. Goldberg GS, Bechberger JF, Naus CCG (1995): A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer. Biotechniques 18:490-497.
14. Greene LA, Tischler AS (1982): PC 12 pheochromocytoma cultures in neurobiological research. In Federoff S, Hertz L (eds): "Advances in Cellular Neurobiology." New York: Academy of Science, pp 373-414.
15. Hartley RS, Trojanowski JO, Lee VMY (1995): Human neuronal Ntera-2 cells implanted into spinal cord mature and integrate along the host architecture independent of host age or location. Abst Soc Neurosci 21:2026.
16. Johnson J (1981): A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Meth 43: 349-350.
17. Kadle R, Zhang JT, Nicholson BJ (1991): Tissue-specific distribution of differentially phosphorylated forms of Cx43. Mol Cell Biol 11:363-369.
18. Kandler K, Katz LC (1995): Neuronal coupling and uncoupling in the developing nervous system. Curr Opin Neurobiol 5:98-105.
19. Kren BT, Kumar NM, Wang SQ, Gilula NB, Steer CJ (1993): Differential regulation of multiple gap junction transcripts and proteins during rat liver regeneration. J Cell Biol 123:707-718.
20. Kurie JM, Brown P, Salk E, Scheinberg D, Birrer M, Deutsch P, Dmitrovsky E (1994): Cooperation between retinoic acid and phorbol esters enhances human teratocarcinoma differentiation. Differentiation 54:115-122.
21. Laird DW, Castillo M, Kasprzak L (1995) Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells. J Cell Biol 131:1193-1203.
22. Lange BJ (1989) Growth of human leukaemia cells in vitro. In Baserga R (ed): "Cell Growth and Division, A Practical Approach." New York: IRL Press. pp 67-68.
23. LaPlaca MC, Djali S, Saatman KE, Thibault LE (1995): Glutamate mediated cell damage in mechanically injured Ntera-2 neurons. Abst Soc Neurosci 21:497.
24. Lee VMY, Andrews PW (1986): Differentiation of Ntera-2 clonal human embryonal carcinoma cells into neurons involves the induction of all three neurofilament proteins. J Neurosci 6:514-521.
25. Mavilio F, Simeone A, Boncinelli E, Andrews PW (1988): Activation of tour homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37:73-79.
26. Micevych PE, Abelson L (1991): Distribution of mRNAs coding for liver and heart gap junction proteins in the rat central nervous system. J Comp Neurol 305:96-118.
27. Musil LS, Goodenough DA (1990): Gap junctional intercellular communication and regulation of connexin expression and function. Curr Opin Cell Biol 2:875-880.
28. Naus CCG, Bechberger JF, Caveney S, Wilson JX (1991): Expression of gap junction genes in astrocytes and C6 glioma cells. Neurosci Lett 126:33-36.
29. Nelles E, Butzler C, Jung D, Temme A, Gabriel HD, Dahl U, Traub O, Stumpel F, Jungermann K, Zielasek J, Toyka KV, Dermietzel R, Willecke K (1996) Defective propagation of signals generated by sympathetic nerve stimulation in the liver of connexin32-deficient mice. Proc Natl Acad Sci USA 93:9565-9570.
30. Nuzzolo L, Vellucci A (1983) Cell viability. In Nuzzolo L (ed): "Tissue Culture Techniques." St. Louis, MO: WH Green Inc. pp 158-167.
31. Oh SY, Dupont E, Madhukar BV, Briand JP, Chang CC, Beyer E, Trosko JE (1993): Characterization of gap junctional communication-deficient mutants of a rat liver epithelial cell line. Eur J Cell Biol 60:250-255.
32. Oosthoek PW, Viragh Sz, Lamers WH, Moorman AFM (1993a): Immunohistochemical delineation of the conduction system II. The atrioventricular node and Purkinje fibers. Circ Res 73:482-491.
33. Oosthoek PW, Viragh Sz, Mayen AEM, van Kempen MJA, Lamers WH, Moorman AFM (1993b): Immunohistochemical delineation of the conduction system. I. The sinoatrial node. Circ Res 73:473-481.
34. Oyamada M, Kimura H, Oyamada Y, Miyamoto A, Ohshika H, Mon M (1994): The expression, phosphoryration, and localization of connexin43 and gap junctional intercellular communication during the establishment of a synchronized contraction of cultured neonatal rat cardiac myocytes. Exp Cell Res 212:315-358.
35. Paul DL (1986): Molecular cloning of cDNA for rat liver gap junction protein. J Cell Biol 103:123-134.
36. Paul DL (1995): New functions for gap junctions. Curr Opin Cell Biol 7:665-672.
37. Peinado A, Yuste R, Katz LC (1993): Extensive dye coupling between rat neocortical neurons during the period of circuit formation. Neuron 10:103-114.
38. Pleasure SJ, Page C, Lee VMY (1992): Pure, postmitotic, polarized human neurons derived from Ntera2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons. J Neurosci 12:1802-1815.
39. Pleasure SJ, Lee VMY (1993): Ntera2 cells: A human cell line which displays characteristics expected of a human committed neuronal progenitor cell. J Neurosci 35:585-602.
40. Puranam KL, Laird DW, Revel JP (1993) Trapping an intermediate form of connexin43 in the Golgi. Exp Cell Res 206:85-92.
41. Reaume AG, De Sousa PA, Kulkarni S, Langille BL, Zhu D, Davies TC, Juneja. SC, Kidder GM, Rossant J (1995): Cardiac malformation in neonatal mice lacking connexin43. Science 267:1831-1834.
42. Ronnett GV, Hester LD, Nye JS, Conners K, Snyder SH (1990): Human cortical neuronal cell line: Establishment from a patient with unilateral megalencephaly. Science 248:603-605.
43. Rozental R, Mehler MF, Morales M, Andrade-Rozental AF, Kessler JA, Spray DC (1995): Differentiation of hippocampal progenitor cells in vitro: Temporal expression of intercellular coupling and voltage- and ligand-gated responses. Dev Biol 167:350-362.
44. Rudnicki MA, McBurney MW (1987) Cell culture methods and induction of differentiation of embryonal carcinoma cell lines. In Robertson EJ (ed): "Teratocarcinomas and Embryonic Stem Cells, A Practical Approach." Washington, DC: IRL Press, pp 19-49.
45. Saez JC, Nairn AC, Czernik AJ, Spray DC, Hertzberg EL (1993): Rat connexin43: regulation by phosphorylation in heart. In Hall JE, Zampighi GA, Davis RM (eds): "Gap Junctions: Progress in Cell Research Vol. 4." Amsterdam: Elsevier. pp 275-281.
46. Sambrook J, Fritsch EF, Maniatis T (1989) Extraction, Purification, analysis of messenger RNA from eukaryotic cells. In: "Molecular Cloning: A Laboratory Manual." 2nd ed., Chap. 7. Cold Springs, NY: Cold Spring Harbor Laboratories.
47. Tang CM, Margulis M, Hartley R, Lee VMY (1995): Synaptogenesis by cells derived from the human NT2 cell line. Abst Soc Neurosci 21:1749.
48. Trojanowski JQ, Hickey WF (1984): Human teratomas express differentiated neural antigens: An immunohistochemical study with antineurofilament, anti-glial filament and anti-myelin basic protein monoclonal antibodies. Am J Pathol 115:383-389.
49. Trojanowski JQ, Mantione JR, Lee JH, Seid DP, You T, Inge LJ, Lee VMY (1993): Neurons derived from a human teratocarcinoma cell line establish molecular and structural polarity following transplantation into the rodent brain. Exp Neurol 122:283-294.
50. Umezawa A, Hata J (1992) Expression of gap junction protein (connexin 43) is down-regulated at the transcriptional level during adipocyte differentiation of H-1/A marrow stromal cells. Cell Struc Func 17:177-184.
51. Van Kempen MJA, Velde IT, Wessels A, Oosthoek PW, Gros D, Jongsma HJ, Moorman AFM, Lamers WH (1995): Differential connexin distribution accommodates cardiac function in different species. Microsc Res Tech 31:420-436.
52. Wertkin AM, Turner RS, Pleasure SJ, Golde TE, Younkin SG, Trojanowski JQ, Lee VMY (1993): Human neurons derived from a teratocarcinoma cell line express solely the 695-amino acid amyloid precursor protein and produce intracellular β-amyloid or A4 peptides. Proc Natl Acad Sci USA 90:9513-9517.
53. Wolburg H, Rohlmann A (1995): Structure-function relationships in gap junctions. In Jeon KW, Jarvik J (eds): "International Review of Cytology," Vol 157. New York: Academic Press, pp 315-373.
54. Yamamoto T, Shiosaka S, Whittaker ME, Hertzberg EL, Nagy JI (1989): Gap junction protein in rat hippocampus: Light microscope immunohistochemical localization. J Comp Neurol 281: 269-281.
55. Younkin DP, Tang CM, Hardy M, Reddy UR, Shi QY, Pleasure SJ, Lee VMY, Pleasure D (1993): Inducible expression of neuronal glutamate receptor channels in the NT2 human cell line. Proc Natl Acad Sci USA 90:2174-2178.
56. Yuste R, Peinado A, Katz LC (1992): Neuronal domains in developing neocortex. Science 257:665-669.
57. Zhang JT, Nicholson BJ (1989): Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA. J Cell Biol 109:3391-3401.