Direct enumeration of bacteria from macroalgae by epifluorescence microscopy as applied to the fleshy red algae Kappaphycus alvarezii and Gracilaria spp. (Rhodophyta)

Journal of Phycology

Volumn 33, Issue 3, 1997, Pages 554-557

  Largo, Danilo B ^a   Fukami, Kimio ^a   Adachi, Masao ^a   Nishijima, Toshitaka ^a  

^a KOCHI UNIVERSITY   (Japan)

Author keywords

Direct counting method; Epifluorescence microscopy; Gracilaria spp.; Kappaphycus alvarezii; Seaweed bacteria

Indexed keywords

ALGAE; BACTERIA (MICROORGANISMS); GRACILARIA; KAPPAPHYCUS ALVAREZII; RHODOPHYTA;

EID: 0030758783     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-3646.1997.00554.x     Document Type: Article

References (14)

1. Atlas, R. M. & Bartha, R. 1987. Microbial Ecology: Fundamentals and Applications. Benjamin/Cummings Publishing Company, Menlo Park, California, 533 pp.
2. Baker, J. H. 1988. Epiphytic bacteria. In Austin, B. [Ed.] Methods in Aquatic Bacteriology. John Wiley & Sons Ltd., Chichester, pp. 171-91.
3. Chan, E. C. S. & McManus, E. A. 1967. Development of a method for the total count of marine bacteria on algae. Can. J. Microbiol. 13:295-301.
4. Coleman, A. W. 1980. Enhanced detection of bacteria in natural environments by fluorochrome staining of DNA. Limnol. Oceanogr. 25:948-51.
5. Fry, J. C. 1988. Determination of biomass. In Austin, B. [Ed.] Methods in Aquatic Bacteriology. John Wiley and Sons Ltd., Chichester, pp. 27-72.
6. Goff, L. J. & Coleman, A. W. 1990. DNA: microspectrofluorometric studies. In Cole, K. M. & Sneath, R. G. [Eds.] Biology of the Red Algae. Cambridge University Press, New York, pp. 43-71.
7. Hobbie, J. E., Daley, R. J. & Jasper, S. 1977. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33:1225-8.
8. Karl, D. M. 1986. Determination of in situ microbial biomass, viability, metabolism, and growth. In Poindexter, J. S. & Leadbetter, E. R. [Eds.] Bacteria in Nature. Plenum Press, New York, pp. 85-176.
9. Kong, M. K. & Chan, K. C. 1979. A study on the bacterial flora isolated from marine algae. Bot. Mar. 22:83-97.
10. Largo, D. B., Fukami, K. & Nishijima, T. 1995a. Occasional pathogenic bacteria promoting ice-ice disease in the carrageenan-producing red algae Kappaphycus alvarezii and Eucheuma denticulatum (Solieriaceae, Gigartinales, Rhodophyta). J. Appl. Phycol. 7:545-54.
11. Largo, D. B., Fukami, K., Nishijima, T. & Ohno, M. 1995b. Notes on the thalli whitening called ice-ice in red algae, Eucheuma/Kappaphycus and Graciaria. Bull. Mar. Sci. Fish. Kochi Univ. 15:39-42.
12. Laycock, R. A. 1974. The detrital food chain based on seaweeds. I. Bacteria associated with the surface of Laminaria fronds. Mar. Biol. 25:224-31.
13. Porter, K. C. & Feig, Y. S. 1980. The use of DAPI for identifying and counting of aquatic microflora. Limnol Oceanogr. 25:943-8.
14. Velji, M. I. & Albright, L. J. 1986. Microscopic enumeration of attached marine bacteria of seawater, marine sediment, fecal matter, and kelp blade samples following pyrophosphate and ultrasound treatments. Can. J. Microbiol. 32:121-6.