Characterization of mutations affecting the Escherichia coli essential GTPase Era that suppress two temperature-sensitive dnaG alleles

Journal of Bacteriology

Volumn 179, Issue 14, 1997, Pages 4575-4582

  Britton, Robert A ^a,c   Powell, Bradford S ^b   Court, Donald L ^b   Lupski, James R ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA PRIMASE; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; MUTANT PROTEIN;

ALLELE; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENE; CELL DIVISION; CONTROLLED STUDY; ENZYME REPRESSION; ESCHERICHIA COLI; GENE MUTATION; GENE REPRESSION; NONHUMAN; OPERON; PRIORITY JOURNAL; TEMPERATURE SENSITIVE MUTANT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030755506     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.14.4575-4582.1997     Document Type: Article

References (34)

1. Ahnn, J., P. E. March, H. E. Takiff, and M. Inouye. 1986. A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins. Proc. Natl. Acad. Sci. USA 83:8849-8853.
2. Altuvia, S., H. Locker-Giladi, S. Koby, O. Ben-Nun, and A. B. Oppenheim. 1987. RNaseIII stimulates the translation of the cIII gene of bacteriophage λ. Proc. Natl. Acad. Sci. USA 84:6511-6515.
3. Bardwell, J. C. A., P. Regnier, S. Chen, V. Nakamura, M. Grunberg-Manago, and D. L. Court. 1989. Autoregulation of RNase III operon by mRNA processing. EMBO J. 8:3401-3407.
4. Bourne, H. R., D. A. Sanders, and F. McCormick. 1991. The GTPase superfamily: conserved structure and molecular mechanism. Nature 349:117-127.
5. Bram, R. J., R. A. Young, and J. A. Steitz. 1980. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli. Cell 19:393-401.
6. 1 that suppress two dnaG alleles in Escherichia coli. Mol. Gen. Genet. 246:729-733.
7. Britton, R. A., and J. R. Lupski. 1997. Isolation and characterization of suppressors of Escherichia coli dnaG mutations, dnaG2903 and parB. Genetics 145:867-875.
8. Chen, S.-M., H. E. Takiff, A. M. Barber, J. C. Dubois, J. C. A. Bardwell, and D. L. Court. 1990. Expression and characterization of RNaseIII and Era proteins. J. Biol. Chem. 265:2888-2895.
9. Cooper, S., and C. E. Helmstetter. 1968. Chromosome replication and the division cycle of Escherichia coli B/R. J. Mol. Biol. 31:519-540.
10. Court, D. 1993. RNA processing and degradation by RNaseIII, p. 71-116. In J. Belasco and G. Brawerman (ed.), Control of messenger RNA stability. Academic Press, San Diego, Calif.
11. Finkel, T., C. J. Der, and G. M. Cooper. 1984. Activation of ras genes in human tumors does not affect localization, modification, or nucleotide binding properties of p21. Cell 37:151-158.
12. Gollop, N., and P. March. 1991. Localization of the membrane binding sites of Era in Escherichia coli. Res. Microbiol. 142:301-307.
13. Gollop, N., and P. E. March. 1991. A GTP-binding protein (Era) has an essential role in growth rate and cell cycle control in Escherichia coli. J. Bacteriol. 173:2265-2270.
14. Grompe, M., J. Versalovic, T. Koeuth, and J. R. Lupski. 1991. Mutations in the Escherichia coli dnaG gene suggest coupling between DNA replication and chromosome partitioning. J. Bacteriol. 173:1268-1278.
15. Hiraga, S., H. Niki, T. Ogura, C. Ichinose, H. Mori, B. Ezaki, and A. Jaffe. 1989. Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells. J. Bacteriol. 171:1496-1505.
16. Inada, T., K. Kawakami, S. Chen, H. Takiff, D. Court, and Y. Nakamura. 1989. Temperature-sensitive lethal mutant of Era, a G protein in Escherichia coli. J. Bacteriol. 171:5017-5024.
17. Katayama, T., Y. Murakami, C. Wada, H. Ohmori, T. Yura, and T. Nagata. 1989. Genetic suppression of a dnaG mutation in Escherichia coli. J. Bacteriol. 171:1485-1491.
18. Koepp, D. M., and P. A. Silver. 1996. A GTPase controlling nuclear trafficking: running the right way of walking RANdomly? Cell 87:1-4.
19. Kohara, Y., K. Akiyama, and K. Isono. 1987. The physical map of the whole Escherichia coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495-508.
20. Kolodner, R., R. A. Fishel, and M. Howard, 1985. Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli. J. Bacteriol. 163:1060-1066.
21. Krengel, U., I. Schlichting, A. Scherer, R. Schumann, M. Frech, J. John, W. Kabsch, E. Pai, and A. Wittinghofer. 1990. Three-dimensional structures of H-ras p21 mutants: molecular basis for their inability to function as signal switch molecules. Cell 62:539-548.
22. Lerner, C. G., and M. Inouye. 1991. Pleiotropic changes resulting from depletion of Era, an essential GTP-binding protein in Escherichia coli. Mol. Microbiol. 5:951-957.
23. Lin, Y. P., J. D. Sharer, and P. E. March. 1994. GTPase-dependent signaling in bacteria: characterization of a membrane-binding site for era in Escherichia coli. J. Bacteriol. 176:44-49.
24. Marshall, C. 1985. Human oncogenes, p. 487-558. In R. Weiss, N. Teich, H. Varmus, and J. Coffin (ed.), RNA tumor viruses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Powell, B. S., D. L. Court, T. Inada, Y. Nakamura, V. Michotey, X. Cui, A. Reizer, M. H. Saier, Jr., and J. Reizer. 1995. Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli J. Biol. Chem. 270:4822-4839.
27. Roa, B., C. A. Garcia, U. Suter, D. A. Kulpa, C. A. Wise, J. Mueller, A. A. Welcher, G. J. Snipes, E. M. Shooter, P. I. Patel, and J. R. Lupski. 1993. Charcot-Marie-Tooth disease type 1A: association with a spontaneous point mutation in the PMP22 gene. N. Engl. J. Med. 329:96-101.
28. Seeburg, P. H., W. W. Colby, D. J. Capon, D. V. Goeddel, and A. D. Levinson. 1984. Biological properties of human c-Ha-ras1 genes mutated at codon 12. Nature 312:71-75.
29. Sood, P., C. G. Lerner, T. Shimamoto, Q. Lu, and M. Inouye. 1994. Characterization of the autophosphorylation of Era, an essential GTPase in Escherichia coli. Mol. Microbiol. 12:201-208.
30. Takiff, H., S.-M. Chen, and D. L. Court. 1989. Genetic analysis of the mc operon of Escherichia coli. J. Bacteriol. 171:2581-2590.
31. Takiff, H. E., T. Baker, T. Copeland, S.-M. Chen, and D. L. Court. 1992. Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon. J. Bacteriol. 174:1544-1553.
32. Versalovic, J., and J. R. Lupski. 1997. Missense mutations in the 3′ end of the Escherichia coli dnaG gene confer the chromosome-segregation-defective (par) phenotype. Microbiology 143:585-594.
33. Walworth, N. C., P. Brennwald, A. K. Kabcenell, M. Garrett, and P. Novick. 1992. Hydrolysis of GTP by Sec4 protein plays an important rote in vesicular tranport and is stimulated by a GTPase-activating protein in yeast. Mol. Cell. Biol. 12:2017-2028.
34. Weinstock, G. 1987. General recombination in Escherichia coli, p. 1034-1043. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.