Novel features of the functional site and expression of the yeast deoxyhypusine synthase

NeuroSignals

Volumn 6, Issue 3, 1997, Pages 157-165

  Abid, Md Ruhul ^a   Ueda, Koji ^a   Miyazaki, Masazumi ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

Deoxyhypusine synthase; Eukaryotic initiation factor 5A; Gene expression; Hypusine; Posttranslational modification; Spermidine

Indexed keywords

AMINO ACID; CYSTEINE; DEOXYHYPUSINE; DEOXYHYPUSINE SYNTHASE; INITIATION FACTOR; INITIATION FACTOR 5A; SYNTHETASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SYNTHESIS; AMINO TERMINAL SEQUENCE; ARTICLE; BIOSYNTHESIS; CARBOXY TERMINAL SEQUENCE; CELL GROWTH; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; GENE FUNCTION; GENE OVEREXPRESSION; GENE REPRESSION; IMMUNOBLOTTING; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PROCESSING; SACCHAROMYCES CEREVISIAE; YEAST CELL;

GENE DELETION; GENES, FUNGAL; OXIDOREDUCTASES ACTING ON CH-NH GROUP DONORS; POINT MUTATION; SACCHAROMYCES CEREVISIAE; STRUCTURE-ACTIVITY RELATIONSHIP;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0030755202     PISSN: 1424862X     EISSN: 14248638     Source Type: Journal    
DOI: 10.1159/000109122     Document Type: Article

References (17)

1. Park MH, Wolff EC, Folk JE: Hypusine: Its post-translational formation of in eukaryotic initiation factor 5A and its potential role in cellular regulation. Biofactors 1993,4:95-104.
2. Park MH, Wolff EC: Cell-free synthesis of deoxyhypusine. J Biol Chem 1988;263:15264-15269.
3. Murphey RJ, Gemer EW: Hypusine formation in protein by a two-step process in cell lysates. J Biol Chem 1987;262:15033-15036.
4. Smit-McBride Z, Schnier J, Kaufman RJ, Hershey JWB: Protein synthesis initiation factor eIF-4D. J Biol Chem 1989;264:18527-18530.
5. Schnier J, Schwelberger HG, Smit-McBride Z, Kang HA, Hershey JWB: Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae. Mol Cell Biol 1991;11:3105-3114.
6. Kang HA, Hershey JWB: Effect of initiation factor elF-5A depletion on protein synthesis and proliferation of Saccharomyces cerevisiae. J Biol Chem 1994;269:3934-3940.
7. Park MH, Wolff EC, Lee YB, Folk JE: Anti-proliferative effects of inhibitors of deoxyhypusine synthase. J Biol Chem 1994:269:27827-27830.
8. Byers TL, Ganem B, Pegg AE: Cy-tostasis induced in L12I0 murine leukaemia cells by the S-adenosyl-L-methioninc decarboxylase inhibitor 5' - ([(Z) - 4 - amiro - 2 - butenyljmethyl-amino)-5'-dcoxyadenosinc may be duetohypusinedeplction. Biochem J 1992;287:717-724.
9. Sasaki K, Abid MR, Miyazaki M: Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cererisiae. FEBS Lett 1996;384:151-154.
10. Abid MR, Sasaki K. Titani K, Miyazaki M: Biochemical and immunological characterization of deoxyhypusine synthase purified from the yeast Saccharomycescarlsbergensis. J Bio-chem 1997;121:769-778.
11. Kohrer K, Domdey H:Preparationof high molecular weight RNA; in Guthrie C, Fink GR (ed): Guide to Yeast Genetics and Molecular Biology. Methods in Enzymology. San Diego, Academic Press, 1991, vol 194, pp 398-405.
12. Kingston RE: Primer extension; in Ausubcl FM (ed): Current Protocols in Molecular Biology. New York. Greene and Wiley-Intcrscience, 1987, vol 1. pp 4.8.1-4.S.3.
13. Kang KR. Wolff EC, Park MH, Folk JE, Chung SI: Identification of YHROóSw in Saccharomyces cerevi-siae chromosome Vili as a gene for deoxyhypusinc synthase: Expression and characterization of the enzyme. J Biol Chem 1995;270:18408-18412.
14. Joe YA, Wolff EC, Park MH: Cloning and expression of human deoxy-hypusine synthase cDNA. J Biol Chem 1995;270:22386-22392.
15. Tao Y, Chen KY: Molecular cloning and functional expression of Neuro-spora deoxyhypusinc synthase cDNA and identification of yeast deoxyhypusinc synthase cDNA. J Biol Chem 1995;270:23984-23987.
16. Tao Y, Chen KY: Purification of deoxyhypusine synthase from Neurospora crassa to homogeneity by substrate elution affinity chromatography. J Biol Chem 1995;270: 383-386.
17. Gordon ED, Mora R, Meredith SC, Lee C, Lindquist SL: Eukaryotic initiation factor 4D, the hypusine-con-taining protein, is conserved among eukaryotes. J Biol Chem 1987,262: 16585-16589.