Functional interaction between Chlamydomonas outer arm dynein subunits: The γ subunit suppresses the ATPase activity of the αβ dimer

Cell Motility and the Cytoskeleton

Volumn 37, Issue 4, 1997, Pages 338-345

  Nakamura, Ken Ichi ^a,b   Wilkerson, Curtis G ^a   Witman, George B ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b Hiroshima Women's University   (Japan)

Author keywords

Dynein assembly; Flagella; Hydrostatic pressure; Other arm dynein purification; Outer dynein arm docking complex; Sucrose gradients

Indexed keywords

ADENOSINE TRIPHOSPHATASE; DYNEIN ADENOSINE TRIPHOSPHATASE;

ANIMAL CELL; ARTICLE; AXONEME; CHLAMYDOMONAS; CONTROLLED STUDY; FLAGELLUM; HYDROSTATIC PRESSURE; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION;

ADENOSINE TRIPHOSPHATASES; ANIMALS; BINDING SITES; CHLAMYDOMONAS REINHARDTII; DIMERIZATION; DYNEIN ATPASE; PROTOZOAN PROTEINS;

ANIMALIA; CHLAMYDOMONAS;

EID: 0030754633     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0169(1997)37:4<338::AID-CM5>3.0.CO;2-0     Document Type: Article

References (27)

1. Bradford, M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
2. Goodenough, U., and Heuser, J. (1984): Structural comparison of purified dynein proteins with in situ dynein arms. J. Mol. Biol. 180:1083-1118.
3. Kamiya, R., Kurimoto, E., and Muto, E. (1991): Two types of Chlamydomonas flagellar mutants missing different components of inner arm dynein. J. Cell Biol. 12:441-447.
4. King, S.M., and Witman, G.B. (1990): Localization of an intermediate chain of outer arm dynein by immunoelectron microscopy. J. Biol. Chem. 265:19807-19811.
5. King, S.M., Wilkerson, C.G., and Witman, G.B. (1991): The Mr. 78,000 intermediate chain of Chlamydomonas outer arm dynein interacts with α-tubulin in situ. J. Biol. Chem. 266:8401-8407.
6. Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
7. Mitchell, D.R., and Rosenbaum, J.L. (1986): Protein-protein interactions in the 18S ATPase of Chlamydomonas outer dynein arm. Cell Motil. Cytoskeleton 6:510-520.
8. Nakamura, K.-I., Masuyama, E., Suzaki, T., and Shigenaka, Y. (1982): Flagellar adenosine triphosphatase from annelid spermatozoa. Arch. Biochem. Biophys. 214:172-179.
9. Pfister, K.K., and Witman, G.B. (1984): Subfractionation of Chlamydomonas 18S dynein into two unique subunits containing ATPase activity. J. Biol. Chem. 259:12072-12080.
10. Pfister, K.K., Fay, R.B., and Witman, G.B. (1982): Purification and polypeptide composition of dynein ATPase from Chlamydomonas reinhardtii. Cell Motil. Cytoskeleton 2:525-547.
11. Piperno, G., and Luck, D.J.L. (1979): Axonemal adenosine triphosphatases from flagella of Chlamydomonas reinhardtii. Purification of two dyneins. J. Biol. Chem. 254:3084-3090.
12. Piperno, G., Ramanis, Z., Smith, E.F., and Sale, W.S. (1990): Three distinct inner dynein arms of Chlamydomonas flagella: Molecular composition and location in the axoneme. J. Cell Biol. 110:379-389.
13. Porter, M.E., Power, J., and Dutcher, S.K. (1992): Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms. J. Cell Biol. 118:1163-1176.
14. Sakakibara, H., Takada, S., King, S.M., Witman, G.B., and Kamiya, K. (1993): A Chlamydomonas outer arm dynein nuitunl with a truncated β heavy chain. J. Cell Biol. 122:653-661.
15. Sale, W.S., and Satir, P. (1977): Direction of active sliding of microtubules in Tetrahymena cilia. Proc. Natl. Acad. Sci. U.S.A. 74:2045-2049.
16. Smith, E.F., and Sale, W.S. (1991): Microtubule binding and translocation by inner dynein arm subtype II. Cell Motil. Cytoskeleton 18:258-268.
17. Summers, K.E., and Gibbons, I.R. (1971): Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm. Proc. Natl. Acad. Sci. U.S.A. 68:3092-3096.
18. Takada, S., and Kamiya, R. (1994): Functional reconstitution of Chlamydomonas outer arm dynein arms from α-β and γ-subunits: Requirement of a third factor. J. Cell Biol. 126:737-745.
19. Takada, S., Sakakibara, H., and Kamiya, R. (1992): Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer-arm-missing axonemes. J. Biochem. 111:758-762.
20. Watanabe, T., and Flavin, M. (1973): Two types of adenosine triphosphatase from flagella of Chlamydomonas reinhardtii. Biochem. Biophys. Res. Commun. 52:195-201.
21. Weber, G., and Drickamer, H.G. (1983): The effect of high pressure upon proteins and other biomolecules. Q. Rev. Biophys. 16:89-112.
22. Witman, G.B. (1986): Isolation of Chlamydomonas flagella and flagellar axonemes. Methods Enzymol. 134:280-290.
23. Witman, G.B. (1990): Introduction to cilia and flagella. In Bloodgood, R.A. (ed.): "Ciliary and Flagellar Membranes." New York: Plenum, pp. 1-30.
24. Witman, G.B. (1992): Axonemal dyneins. Curr. Opin. Cell Biol. 4:74-79.
25. Witman, G.B., Johnson, K.A., Pfister, K.K., and Wall, J.S. (1983): Fine structure and molecular weight of the outer arm dyneins of Chlamydomonas. J. Submicrosc. Cytol. 15:193-197.
26. Witman, G.B., King, S.M., Moss, A.G., and Wilkerson, C. (1991): The intermediate chain/light chain complex - an important structural entity of outer arm dynein. In Baccetti, B. (ed.): "Comparative Spermatology 20 Years After." New York: Raven, pp. 439-443.
27. Witman, G.B., Wilkerson, C.G., and King, S.M. (1994): The biochemistry, genetics, and molecular biology of flagellar dynein. In Hyams, J.S., and Lloyd, C.W. (eds): "Microtubules." New York: Wiley-Liss, pp. 229-249.