Control of PHAS-I phosphorylation in 3T3-L1 adipocytes: Effects of inhibiting protein phosphatases and the p70(S6K) signalling pathway

Diabetologia

Volumn 40, Issue SUPPL. 2, 1997, Pages

  Lin, T A ^a   Lawrence Jr , J C ^a,b  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Calyculin A; Insulin; mRNA translation; Okadaic acid; Rapamycin; Wortmannin

Indexed keywords

INSULIN;

CONFERENCE PAPER; DIABETES MELLITUS; HUMAN; INSULIN METABOLISM; MITOGENESIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; SIGNAL TRANSDUCTION; TRANSLATION INITIATION; TRANSLATION REGULATION;

3T3 CELLS; ANDROSTADIENES; ANIMALS; CARRIER PROTEINS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME INHIBITORS; EUKARYOTIC INITIATION FACTOR-4E; FIBROBLASTS; IMMUNE SERA; IMMUNOBLOTTING; INSULIN; MICE; OKADAIC ACID; OXAZOLES; PEPTIDE INITIATION FACTORS; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOPROTEINS; PHOSPHORYLATION; POLYENES; RABBITS; REPRESSOR PROTEINS; RIBOSOMAL PROTEIN S6 KINASES; SIROLIMUS;

EID: 0030754490     PISSN: 0012186X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001250051391     Document Type: Conference Paper

References (42)

1. Kimball SR, Vary TC, Jefferson LS (1994) Regulation of protein synthesis by insulin. Ann Rev Physiol 56: 321-348
2. O'Brien RM, Granner DK (1991) Regulation of gene expression by insulin. Biochem J 278: 609-619
3. DePhilip RM, Rudert WA, Lieberman I (1980) Preferential stimulation of ribosomal protein synthesis by insulin and in the absence of ribosomal and messenger ribonucleic acid formation. Biochemistry 19: 1662-1669
4. Hammond ML, Bowman LH (1988) Insulin stimulates the translation of ribosomal proteins and the transcription of rDNA in mouse myoblasts. J Biol Chem 263: 17785-17791
5. Manzella JM, Rychlik W, Rhoads RE, Hershey JW, Blackshear PJ (1991) Insulin induction of ornithine decarboxylase. J Biol Chem 266: 2383-2389
6. Jefferies HBJ, Reinhard C, Kozma SC, Thomas G (1994) Rapamycin selectively represses translation of the "polypyrimidine tract" mRNA family. Proc Natl Acad Sci USA 91: 4441-4445
7. Proud CG (1992) Protein phosphorylation in translational control. Curr Top Cellul Regul 32: 243-369
8. Avruch J, Alexander MC, Palmer JL et al. (1982) Role of insulin-stimulated protein phosphorylation in insulin action. Federation Proc 41: 2629-2633
9. Denton RM, Brownsey RW, Belsham GJ (1981) A partial view of the mechanism of insulin action. Diabetologia 21: 347-362
10. Hu C, Pang S, Kong X, Velleca M, Lawrence JC, Jr (1994) Molecular cloning and tissue distribution of PHAS-I, an intracellular target for insulin and growth factors. Proc Natl Acad Sci USA 91: 3730-3734
11. Graves LM, Bornfeldt KE, Argast GM, Krebs EG, Kong X, Lin T-A, Lawrence JC, Jr (1995) cAMP- and rapamycin-sensitive regulation of the association of eukaryotic initiation factor 4 E and the translational regulator. PHAS-I, in aortic smooth muscle cells. Proc Nat Acad Sci USA 92: 7222-7226
12. Lin T-A, Kong X, Saltiel AR, Blackshear PJ, Lawrence JC, Jr (1995) Control of PHAS-I by insulin in 3T3-L1 adipocytes: synthesis, degradation, and phosphorylation by a rapamycin-sensitive and MAP kinase-independent pathway. J Biol Chem 270: 18531-18538
13. Lin T-A, Kong X, Haystead TAJ, Pause A, Belsham GJ, Sonenberg N, Lawrence JC, Jr (1994) PHAS-I as a link between the MAP kinase and translation initiation. Science 266: 653-656
14. Pause A, Belsham GJ, Gingras AC, Donze O, Lin T-A, Lawrence JC, Jr., Sonenberg N (1994) Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5′ cap function. Nature 371: 762-767
15. Frederickson RM, Sonenberg N (1992) Signal transduction and regulation of translation initiation. Seminar in Cell Biol 3: 107-115
16. Hershey JWB (1991) Translational control in mammalian cells. Ann Rev Biochem 60: 717-755
17. Rhoads RE (1991) Protein synthesis, cell growth and oncogenesis. Current Opinion in Cell Biology 3: 1019-1024
18. Shatkin AJ (1987) mRNA caps-old and newer hats. Bioessays 7: 275-277
19. Lawrence JC, Jr, Lin T-A, Kong X, Haystead TAJ, Hu C (1995) PHAS-I: A new player in the regulation of protein synthesis by insulin and growth factors. In: Baba S. Kaneko T (eds) Diabetes 1994. Elsevier Science, Amsterdam pp 622-629
20. Gallic DR, Traugh JA (1994) Serum and insulin regulate cap function in 3T3-L1 cells. J Biol Chem 269: 7174-7179
21. Haystead TAJ, Haystead CMM, Hu C, Lin T-A, Lawrence JC, Jr (1994) Phosphorylation of PHAS-I by milogen-activated protein (MAP) kinase. Identification of a site phosphorylated by MAP kinase in vitro and in response to insulin in adipocytes. J Biol Chem 269: 23185-23191
22. S6k activation mediated by phosphatidylinositol-3-OH kinase. Nature 370: 71-75
23. Fingar DC, Hausdorff SF, Blenis J, Birnbaum MJ (1993) Dissociation of pp70 ribosomal protein S6 kinase from insulin-stimulated glucose transport in 3T3-L1 adipocytes. J Biol Chem 268: 3005-3008
24. Price DJ, Grove JR, Calvo V, Avruch J, Bierer BE (1992) Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase. Science 257: 973-977
25. Robinson LJ, Razzack ZF, Lawrence JC, Jr., James DE (1993) Mitogen-activated protein kinase activation is not sufficient for stimulation of glucose transport or glycogen synthase in 3T3-L1 adipocytes. J Biol Chem 268: 26422-26427
26. Cohen P (1991) Classification of protein-serine/threonine phosphatases: identification and quantitation in cell extracts. Methods Enzymol 201: 389-398
27. Smith PK, Krohn RI, Hermanson GT et al. (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85
28. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature Lond 227: 680-685
29. Cheatham B, Vlahos CJ, Cheatham L, Wang L, Blenis J, Kahn CR (1994) Phosphatidylinositol 3-kinase activation is required for insulin stimulation of pp70 S6 kinase, DNA synthesis, and glucose transporter translocation. Molec Cell Biol 14: 4902-4911
30. Schreiber SL (1991) Chemistry and biology of the immunophilins and their immunosuppressive ligands. Science 251: 283-287
31. Kunz J, Hall MN (1993) Cyclosporin A, FK506 and rapamycin: more than just immunosuppression. Trends Biochem Sci 18: 334-338
32. Clardy J (1995) The chemistry of signal transduction. Proc Natl Acad Sci USA 92: 56-61
33. Brown EJ, Albers MW, Shin TB et al. (1994) A mammalian protein targeted by G1 arresting rapamycin-receptor complex. Nature 369: 756-758
34. Sabatini DM, Erdjument-Bromage H, Lui M, Tempst P, Snyder SH (1994) RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell 78: 35-43
35. Sabers CJ, Martin MM, Brunn GJ et al.(1995) Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. J Biol Chem 270: 815-822
36. Chung J, Kuo CJ, Crabtree GR, Blenis J (1992) Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases. Cell 69: 1227-1236
37. s6k through dephosphorylation of a novel set of sites. J Biol Chem 268: 16091-16094
38. s6k is associated with phosphorylation of four clustered sites displaying Ser/Thr-Pro motifs. Proc Natl Acad Sci U S A 89: 7282-7286
39. De Benedetti A, Rhoads RE (1990) Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrant growth and morphology. Proc Natl Acad Sci USA 87: 8212-8216
40. Lazaris-Karatzas A, Montine KS, Sonenberg N (1990) Malignant transformation by a eukaryotic initiation factor subunit that binds to mRNA 5′ cap. Nature 345: 544-547
41. r-22,000 protein in 3T3-L1 adipocytes. Biochem J 214: 11-19
42. Cohen P, Holmes CF, Tsukitani Y (1990) Okadaic acid: a new probe for the study of cellular regulation. Trends Biochem Sci 15: 98-102