Actin in the parasite toxoplasma gondii is encoded by a single copy gene, act1 and exists primarily in a globular form

Cell Motility and the Cytoskeleton

Volumn 37, Issue 3, 1997, Pages 253-262

  Dobrowolski, Janice M ^a   Niesman, Ingrid R ^a   Sibley, L David ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cytoskeleton; Invasion; Microfilament; Motility; Parasite

Indexed keywords

ACTIN;

ARTICLE; CELL MOTILITY; GENE FUNCTION; NONHUMAN; PATHOGENICITY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; TOXOPLASMA GONDII;

ACTINS; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE EXPRESSION REGULATION; GENES, PROTOZOAN; GENETIC CODE; GENOME, PROTOZOAN; GLOBULINS; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TOXOPLASMA;

CRYPTOSPORIDIUM PARVUM; MAMMALIA; PLASMODIUM FALCIPARUM; PROTOZOA; TOXOPLASMA GONDII; VERTEBRATA;

EID: 0030754072     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0169(1997)37:3<253::AID-CM7>3.0.CO;2-7     Document Type: Article

References (35)

1. Burg, J.L., Perlman, D., Kasper, L.H., Ware, P.L., and Boothroyd, J.C. (1988): Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J. Immunol. 141:3584-3591.
2. Cao, L., Fishkind, D.J., and Wang, Y. (1993): Localization and dynamics of nonfilamentous actin in cultured cells. J. Cell Biol. 123:173-181.
3. Cintra, W.M., and De Souza, W. (1985): Immunocytochemical localization of cytoskeletal proteins and electron microscopy of detergent extracted tachyzoites of Toxoplasma gondii. J. Submicrosc. Cytol. 17:503-508.
4. Cleveland, D.W., Lopata, M.A., MacDonald, R.J., Cowan, N.J., Rutter, W.J., and Kirshner, M.W. (1980): Number and evolutionary conservation of alpha-and beta-tubulin and cytoplasmic beta-and gamma-actin genes using specific cloned DNA probes. Cell 20:95-105.
5. Cook, R.K., Scheff, D.R., and Rubenstein, P.A. (1991): Unusual metabolism of the yeast actin amino terminus. J. Biochem. 266:16825-16833.
6. Cooper, J.A. (1987): Effects of cytochalasin and phalloidin on actin. J. Cell Biol. 105:1473-1478.
7. Dobrowolski, J.M., and Sibley, L.D. (1996): Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite. Cell 84:933-939.
8. Endo, T., Yugita, K., Yasuda, T., and Nakamura, T. (1988): Detection and localization of actin in Toxoplasma gondii. Parasitol. Res. 75:102-106.
9. Erba, H.P., Eddy, R., Shows, Y., Kedes, L., and Gunning, P. (1988): Structure, chromosome location and expression of the human γ-actin gene: Differential evolution, location and expression of the cytoskeletal β-and γ-actin genes. Mol. Cell. Biol. 8:1775-1789.
10. Kabsch, W., Mannherz, H.G., D., S., Pai, E.F., and Holmes, K.C. (1990): Atomic structure of actin: DNAseI complex. Nature 347:37-43.
11. Kurpova, T.S., Tatchell, K., and Cooper, J.A. ( 1995): Actin filaments in yeast are unstable in the absence of capping protein or fimbrin. J. Cell Biol. 131:1483-1493.
12. Kim, K., Gooze, L., Petersen, C., Gut, J., and Nelson, R.G. (1992): Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidum parvum. Molec. Biochem. Parasitol. 50:105-114.
13. Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
14. Lessard, J.L. (1988): Two monoclonal antibodies to actin: One muscle selective and one generally reactive. Cell Motil. Cytoskel. 10:349-362.
15. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982): "Molecular Cloning, A Laboratory Manual." Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
16. Miller, L.H., Aikawa, M., Johnson, J.G. and Shiroishi, T. (1979): Interaction between cytochalasin B-treated malarial parasites and erythrocytes. J. Exp. Med. 149:172-184.
17. Morisaki, J.H., Heuser, J.E., and Sibley, L.D. (1995): Invasion of Toxoplasma gondii occurs by active penetration of the host cell. J. Cell Sci. 108:2457-2464.
18. Nakajima-Iijima, S., Hamada, H., Reddy, P., and Kakunaga, T. (1985): Molecular structure of the human cytoplasmic b-actin gene: Interspecies homology of sequences in the introns. Proc. Natl. Acad. Sci., USA 82:6133-6137.
19. Nichols, B.A., and O'Connor, R.G. (1981): Penetration of mouse peritoneal macrophages by the protozoan Toxoplasma gondii. Lab. Invest. 44:324-335.
20. Pearson, W.R., and Lipman, D.J. (1988): Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444-2448.
21. Pfefferkorn, E.R., and Pfefferkorn, L.C. (1976): Arabinosyl nucleosides inhibit Toxoplasma gondii and allow the selection of resistant mutants. J. Parasitol. 62:993-999.
22. Rubenstein, P.A. (1990): The functional importance of multiple actin isoforms. BioEssays 12:309-315.
23. Russell, D.G. (1983): Host cell invasion by Apicomplexa: An expression of the parasite's contractile system? Parasitology 87:199-209.
24. Russell, D.G., and Sinden, R.E. (1981): The role of the cytoskeleton in the motility of coccidian sporozoites. J. Cell Sci. 50:345-359.
25. Russell, D.G., and Sinden, R.E. (1982): Three-dimensional study of the intact cytoskeleton of coccidian sporozoites. Int. J. Parasitlol. 12:221-226.
26. Ryning, F.W., and Remington, J.S. (1978): Effect of cytochalasin D on Toxoplasma gondii cell entry. Infect. Immun. 20:739-743.
27. Schwartzman, J.D., and Pfefferkorn, E.R. (1983): Immunofluorescent localization of myosin at the anterior pole of the coccidian, Toxoplasma gondii. J. Protozool. 30:657-661.
28. Sibley, L.D., and Boothroyd, J.C. (1992): Construction of a molecular karyotype for Toxoplasma gondii. Molec. Biochem. Parasitol. 51:291-300.
29. Silva, S.R.L., Meirelles, S.S.L., and De Souza, W. (1982): Mechanism of entry of Toxoplasma gondii into vertebrate cells. J. Submicrosc. Cytol. 14:471-482.
30. Smith, D.B., and Johnson, K.S. (1988): Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40.
31. Werk, R. (1985): How does Toxoplasma gondii enter host cells? Rev. Infect. Dis. 7:449-457.
32. Wesseling, J.G., de Ree, J.M., Ponnudurai, T., Smits, M.A. and Schoenmakers. J.G.G. (1988a): Nucleotide sequence and deduced amino acid sequence of a Plasmodium falciparum actin gene. Molec. Biochem. Parsitol. 27:313-320.
33. Wesseling, J.G., Smits, M.A., and Schoenmakers (1988b): Extremely diverged actin proteins in Plasmodium falciparum. Molec. Biochem. Parsitol. 30:143-154.
34. Wesseling, J.G., Snijders, P.J.F., van Someren, P., Jansen, J., Smits, M.A., and Schoenmakers, J.G.G. (1989): Stage-specific expression and genomic organization of the actin genes of the malaria parasite Plasmodium falciparum. Molec. Biochem. Parisitol. 35:167-176.
35. Yasuda, T., Tagita, K., Nakamura, T. and Endo, T. (1988): Immunocytochemical localization of actin in Toxoplasma gondii. Parasitol. Res. 75:107-113.