Three-dimensional microscopy in thick biological samples: A fresh approach for adjusting focus and correcting spherical aberration

Bioimaging

Volumn 5, Issue 1, 1997, Pages 40-49

  Kam, Z ^a   Agard, D A ^b,c   Sedat, J W ^b  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

3 D microscopy; Spherical aberration; Thick specimen

Indexed keywords

ARTICLE; LENS; PRIORITY JOURNAL; THICKNESS; THREE DIMENSIONAL IMAGING;

EID: 0030754048     PISSN: 09669051     EISSN: None     Source Type: Journal    
DOI: 10.1002/1361-6374(199703)5:1<40::AID-BIO4>3.3.CO;2-N     Document Type: Article

References (4)

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2. Scalettar B A, Swedlow J R, Sedat J W and Agard D A 1996 Dispersion, aberration and deconvolution in multi-wavelength fluorescence images J. Microsc. 182 50-60
3. Shaw P J and Rawlins D J 1991 The point-spread function of a confocal microscope: its measurement and use in deconvolution of 3-D data J. Microsc. 163 151-66
4. Sheppard C Jr and Cogswell C J 1991 Effects of aberrating layers and tube length on confocal imaging properties Optik 87 34-8