Phosphorylation of the G protein α-subunit, Gα2, of Dictyostelium discoideum requires a functional and activated Gα2

Journal of Cellular Biochemistry

Volumn 66, Issue 2, 1997, Pages 268-276

  Gundersen, Robert E ^a  

^a UNIVERSITY OF MAINE   (United States)

Author keywords

CAMP; Dictyostelium discoideum; G 2; Phosphorylation; S113A

Indexed keywords

CHEMOATTRACTANT; CYCLIC AMP; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; PROTEIN SUBUNIT;

ALPHA CHAIN; ARTICLE; CHIMERA; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; HYDROLYSIS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

ANIMALS; DICTYOSTELIUM; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); MUTAGENESIS, SITE-DIRECTED; PHENOTYPE; PHOSPHORYLATION; RECEPTORS, CYCLIC AMP; RECOMBINANT FUSION PROTEINS;

DICTYOSTELIUM DISCOIDEUM;

EID: 0030753661     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(19970801)66:2<268::AID-JCB13>3.0.CO;2-D     Document Type: Article

References (46)

1. Ashworth JM, Watts DJ (1970): Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture. Biochem J 119:175-182.
2. sα reveal a new region important for its activation of adenylyl cyclase. FEBS Lett 340:249-254.
3. Bominaar AA, Van Haastert PJM (1994): Phospholipase C in Dictyostelium discoideum: Identification of stimulatory and inhibitory surface receptors and G-proteins. Biochem J 297:189-193.
4. Brazill DT, Gundersen RE, Gomer RH (1997): A cell-density sensing factors regulates the lifetime of a chemoattractant-induced Gα-GTP conformation. FEBS Lett 404: 100-104.
5. Brenner M, Thorns SD (1984): Caffeine blocks activation of cyclic AMP synthesis in Dictyostelium discoideum. Dev Biol 101:136-146.
6. i2 α-subunit phosphorylation in intact hepatocytes. Biochem J 268:449-457.
7. i in human platlets. J Biol Chem 264:13298-13305.
8. Chen MY, Devreotes PN, Gundersen RE (1994): Serine 113 is the site of receptor-mediated phosphorylation of the Dictyostelium G protein α-subunit Gα2. J Biol Chem 269:20925-20930.
9. Chen MY, Insall RH, Devreotes PN (1996): Signaling through chemoattractant receptors in Dictyostelium. Trends Genet 12:52-57.
10. iα1 and the mechanism of GTP hydrolysis. Science 265: 1405-1412.
11. Conklin RB, Bourne HR (1993): Structural elements of Gα subunits that interact with Gβγ, receptors, and effectors. Cell 73:631-641.
12. Devreotes P, Fontana D, Klein P, Sherring J, Theibert A (1987): Transmembrane signaling in Dictyostelium. Methods Cell Biol 28:299-331.
13. Drayer AL, Van der Kaay J, Mayr GW, Van Haastert PJM (1994): Role of phospholipase C in Dictyostelium: Formation of inositol 1,4,5-trisphosphate and normal development in cells lacking phospholipase C activity. EMBO J 13:1601-1609.
14. zα by protein kinase C blocks interaction with the βγ complex. J Biol Chem 270:23119-23125.
15. Gilman AG (1987): G proteins: transducers of receptor-generated signals. Annu Rev Biochem 85:615-649.
16. sα. J Biol Chem 264:15475-15482.
17. Gross JD (1994): Developmental decisions in Dictyostelium discoideum. Microbiol Rev 58:330-351.
18. Gundersen RE, Devreotes PN (1990): In vivo receptor-mediated phosphorylation of a G-protein in Dictyostelium. Science 248:591-593.
19. Katada T, Gilman AG, Watanabe Y, Bauer S, Jakobs KH (1985): Protein kinase C phosphorylates the inhibitory guanine-nucleotide-binding regulatory component and apparently suppresses its function in hormonal inhibition of adenlate cyclase. Eur J Biochem 151:431-437.
20. Klein P, Knox B, Borleis J, Devreotes PN (1987): Purification of the surface cAMP receptor in Dictyostelium. J Biol Chem 262:352-357.
21. Kumagai A, Pupillo M, Gundersen R, Miake-Lye R, Devreotes PN, Firtel RA (1989): Regulation and function of Gα protein subunits in Dictyostelium. Cell 57:265-275.
22. Kunkle TA, Roberts JD, Zakour RA (1987): Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154:367-383.
23. Lambright DG, Noel JP, Hamm HE, Sigler PB (1994): Structural determinants for activation of the α-subunit of a heterotrimeric G protein. Nature 369:621-628.
24. Lambright DG, Sondek J, Bohm A, Skiba NP, Hamm HE, Sigler PB (1996): The 2.0A crystal structure of a heterotrimeric G protein. Nature 379:311-319.
25. Lee E, Taussig R, Gilman AG (1992): The G226A mutant of Gs-α highlights the requirement for dissociation of G-protein subunits. J Biol Chem 267:1212-1218.
26. Lilly PJ, Devreotes PN (1994): Identification of CRAC, a cytosolic regulator required for guanine nucleotide stimulation of adenylyl cyclase in Dictyostelium. J Biol Chem 269:14123-14129.
27. Lilly P, Wu LJ, Welker DL, Devreotes PN (1993): A G-protein β-subunit is essential for Dictyostelium development. Genes Dev 7:986-995.
28. Lounsbury KM, Casey PJ, Brass LF, Manning DR (1991): Phosphorylation of Gz in human platelets. J Biol Chem 266:22051-22056.
29. Milne JLS, Caterina MJ, Devreotes PN (1997): Random mutagenesis of the chemoattractant receptor, cAR1, of Dictyostelium: Evidence for multiple states of activation. J Biol Chem 272:2069-2076.
30. iα1 induced by GTP hydrolysis. Science 270: 954-960.
31. Neer EJ (1995): Heterotrimeric G proteins: Organizers of transmembrane signals. Cell 80:249-257.
32. Okaichi K, Cubitt AB, Pitt GS, Firtel RA (1992): Amino acid substitutions in the Dictyostelium Gα-subunit Gα2 produce dominant negative phenotypes and inhibit the activation of adenylyl cyclase, guanylyl cyclase, and phospholipase-C. Mol Biol Cell 3:735-747.
33. Pyne NJ, Freissmuth M, Pyne S (1992): Phosphorylation of the recombinant spliced variants of the a subunit of the stimulatory guanine nucleotide binding regulatory protein (Gs) by the catalytic subunit of protein kinase A. Biochem Biophys Res Commun 186:1081-1086.
34. Root P, Gundersen RE (1997): Myristoylation of Gα2-subunit in Dictyostelium is required for cell aggregation. In preparation.
35. Sagi-Eisenberg R (1989): GTP-binding proteins as a possible target for protein kinase C action. Trends Biochem Sci 14:355-357.
36. Simon MI, Strathmann MP, Gautam N (1991): Diversity of G proteins in signal transduction. Science 252:802-808.
37. oα. J Biol Chem 268:21889-21894.
38. Snaar-Jagalska BE, Van-Haastert PJM (1994): G-protein assays in Dictyostelium. Methods Enzymol 237:387-408.
39. iα2 attenuates inhibitory adenylyl cyclase in neuroblastoma/ glioma hybrid (NG-108-15) cells. J Biol Chem 269:14307-14313.
40. Van Haastert PJM (1985): The modulation of cell surface cAMP receptors from Dictyostelium discoideum by ammonium sulfate. Biochim Biophys Acta 845:254-260.
41. iα1β1γ2. Cell 83:1047-1058.
42. Wedegaertner PB, Wilson PT, Bourne HR (1995): Lipid modifications of trimeric G proteins. J Biol Chem 270:503-506.
43. Wu LJ, Valkema R, van Haastert PJM, Devreotes PN (1995): The G protein β subunit is essential for multiple responses to chemoattractants in Dictyostelium. J Cell Biol 129:1667-1675.
44. Yuen IS, Taphouse C, Halfant KA, Gomer RH (1991): Regulation and processing of a secreted protein that mediates sensing of cell density in Dictyostelium. Development 113:1375-1385.
45. Yuen IS, Jain R, Bishop JD, Lindsey DF, Deery WJ, van Haastert PJM, Gomer RH (1995): A density-sensing factor regulates signal transduction in Dictyostelium. J Cell Biol 129:1251-1262.
46. Zick Y, Sagi-Eisenberg R, Pines M, Gierschik P, Spiegel AM (1986): Multisite phosphorylation of the α subunit of transducin by the insulin receptor kinase and protein kinase C. Proc Natl Acad Sci USA 83:9294-9297.