Cloning, purification, and preliminary characterization by circular dichroism and NMR of a carboxyl-terminal domain of the bacteriophage p22 scaffolding protein

Protein Science

Volumn 6, Issue 7, 1997, Pages 1583-1586

  Parker, Matthew H ^a   Jablonsky, Michael ^b   Casjens, Sherwood ^c   Sampson, Laura ^c   Krishna, N Rama ^b,d   Prevelige Jr , Peter E ^a,b  

^a University of Alabama at Birmingham   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c UNIVERSITY OF UTAH HEALTH SCIENCES CENTER   (United States)

^d UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

NMR; P22; Scaffolding protein; Virus assembly

Indexed keywords

COAT PROTEIN; VIRUS PROTEIN;

ARTICLE; BACTERIOPHAGE P22; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; MOLECULAR CLONING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN SECONDARY STRUCTURE; VIRUS ASSEMBLY;

DNA VIRUSES; UNIDENTIFIED BACTERIOPHAGE;

EID: 0030752503     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060722     Document Type: Article

References (30)

1. Beaudet-Miller M, Zhang R, Durkin J, Gibson W, Kwong A, Hong Z. 1996. Virus-specific interaction between the human cytomegalovirus major capsid protein and the C terminus of the assembly protein precursor. J Virol 70:8081-8088.
2. Berger B. Shor PW, Tucker-Kellogg L, King J. 1994. Local rule-based theory of virus shell assembly. Proc Natl Acad Sci U.S.A. 91:7732-7736.
3. Berget P. 1985. Pathways in viral morphogenesis. In: Casjens S, ed. Virus structure and assembly. Boston: Jones and Bartlett. pp 149-168.
4. Casjens S. 1985. Nucleic acid packaging by viruses. In: Casjens S, ed. Virus structure and assembly. Boston: Jones and Bartlett. pp 75-147.
5. Casjens S, Adams M. 1985. Posttranscriptional modulation of bacteriophage P22 scaffolding protein gene expression. J Virol 53:185-191.
6. Casjens S, Adams M, Hall C, King J. 1985. Assembly-controlled autogenous modulation of bacteriophage P22 scaffolding protein gene expression. J Virol 53:174-181.
7. Casjens S, Hendrix R. 1988. Control mechanisms in dsDNA bacteriophage assembly. In: Calendar R, ed. The bacteriophages, Vol. 1. New York: Plenum Press, pp 1-91.
8. D'Halluin JC, Martin GR, Torpier G, Boulanger PA. 1978. Adenovirus type 2 assembly analyzed by reversible cross-linking of labile intermediates. J Virol 26:357-363.
9. Eppler K, Wyckoff E, Goates J, Parr R, Casjens S. 1991. Nucleotide sequence of the bacteriophage P22 genes required for DNA packaging. Virology 183:519-538.
10. Flinta C, Persson B, Jörnvall H, von Heijne G. 1986. Sequence determinants of cytosolic N-terminal protein processing. Eur J Biochem 154:193-196.
11. Fuller MT, King J. 1982. Assembly in vitro of bacteriophage P22 procapsids from purified coat and scaffolding subunits. J Mol Biol 156:633-665.
12. Gill S, von Hippel P. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182:319-326.
13. Grcis KD, Hayes BK, Comer FI, Kirk M, Barnes S, Lowary TL, Hart GW. 1996. Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectroscopy. Anal Biochem 234:38-49.
14. Hong Z, Beaudet-Miller M, Durkin J, Zhang R, Kwong AD. 1996. Identification of a minimal hydrophobic domain in the herpes simplex virus type 1 scaffolding protein which is required for interaction with the major capsid protein. J Virol 70:533-540.
15. King J, Casjens S. 1974. Catalytic head assembling protein in virus morphogenesis. Nature 251:112-119.
16. King J, Lenk EV, Botstein D. 1973. Mechanism of head assembly and DNA encapsulation in Salmonella phage P22. II. Morphogenetic pathway. J Mol Biol 80:697-731.
17. Lee JY, Irmiere A, Gibson W. 1988. Primate cytomegalovirus assembly: Evidence that DNA packaging occurs subsequent to B capsid assembly. Virology 167:87-96.
18. Miller JH. 1992. A short course in bacterial genetics. Plainview, New York: Cold Spring Harbor Laboratory Press.
19. Parker MH, Stafford WF III, Prevelige PE Jr. 1997. Bacteriophage P22 scaffolding protein forms oligomers in solution. J Mol Biol 268:655-665.
20. Prevelige PE Jr., King J. 1993. Assembly of bacteriophage P22: A model for ds-DNA virus assembly. Prog Med Virol 40:206-221.
21. Prevelige PE Jr., Thomas D, King J. 1988. Scaffolding protein regulates the polymerization of P22 coat subunits into icosahedral shells in vitro. J Mol Biol 202:743-757.
22. Schultze J., Silhavy T, Berman M, Fiil N. Emr S. 1982. A previously unidentified gene in the spc operon of Escherichia coli K-12 specifies a component of the protein export machinery. Cell 31:221-235.
23. Shaw G, Stonehouse J. 1996. Removing unwanted signals from HSQC spectra. J Magn Reson 110B:91-95.
24. Sherman G, Bachenheimer SL. 1988. Characterization of intranuclear capsids made by ts morphogenic mutants of HSV-1. Virology 163:471-480.
25. Studier W, Rosenberg A, Dünn J, Dubendorff J. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185:60-89.
26. Teschke C, King J, Prevelige PE Jr. 1993. Inhibition of viral capsid assembly by 1,1′-bi(4-anilinonaphthalene-5-sulfonic acid). Biochemistry 32:10658-10665.
27. Tuma R., Prevelige PE Jr, Thomas GJ Jr. 1996. Structural transitions in the scaffolding and coat proteins of P22 virus during assembly and disassembly. Biochemistry 35:4619-4627.
28. Wishart D, Sykes B, Richards F. 1991. Relationship between nuclear magnetic resonance chemical shift and protein secondary structure. J Mol Biol 222:311-333.
29. Wyckoff E, Casjens S. 1985. Autoregulation of the bacteriophage P22 scaffolding protein gene. J Virol 55:192-197.
30. Yang JT, Wu C-SC, Martinez HM. 1986. Calculation of protein conformation from circular dichroism. Methods Enzymol 150:208-269.