Double-pulse fluorescence lifetime measurements

Journal of Microscopy

Volumn 186, Issue 3, 1997, Pages 212-220

  Buist, A H ^a   Muller M ^a   Gijsbers, E J ^a   Brakenhoff, G J ^a   Sosnowski, T S ^b   Norris, T B ^b   Squier, J ^c  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b UNIVERSITY OF MICHIGAN   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

CCD; Confocal microscopy; Fluorescence lifetime imaging; Fluorescence relaxation; Saturation

Indexed keywords

FLUORESCENCE IMAGING; FLUOROPHORES; LASER EXCITATION; OPTICAL PARAMETRIC AMPLIFIERS; PARAMETRIC AMPLIFIERS;

AMPLIFIER SYSTEMS; DOUBLE PULSE; FLUORESCENCE LIFETIME IMAGING; FLUORESCENCE LIFETIME MEASUREMENTS; FLUORESCENCE LIFETIMES; FLUORESCENCE RELAXATION; LASER SOURCES; PICOSECOND TIME SCALE; SATURATION; TIME SCALE RANGE;

CONFOCAL MICROSCOPY;

ARTICLE; CONFOCAL MICROSCOPY; FLUORESCENCE; HALF LIFE TIME; IMAGING SYSTEM; PRIORITY JOURNAL;

EID: 0030750060     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1997.2090773.x     Document Type: Article

References (22)

1. Anderson, S.R. (1991) Time-resolved fluorescence spectroscopy. J. Biol. Chem. 266, 11405-11408.
2. Brakenhoff, G.J. & Visscher, K. (1992) Confocal imaging with bilateral scanning and array detectors. J. Microsc. 165, 139-146.
3. Brakenhoff, G.J. & Visscher, K. (1993) Imaging modes for bilateral confocal scanning microscopy. J. Microsc. 171, 17-26.
4. Draxler, S., Lippitsch, M.E. & Leiner, M.J.P. (1993) Optical pH sensors using fluorescence decay time. Sens. Act. B. 11, 421-424.
5. Gadella, T.W.J. Jr, Clegg, R.M. & Jovin, T.M. (1994) Fluorescence lifetime imaging microscopy: pixel-by-pixel analysis of phase-modulation data. Bioimaging, 2, 139-159.
6. Ghauharali, R.I., Müller, M., Buist, A.H., Sosnowski, T.S., Squier, J. & Brakenhoff, G.J. (1996) Optical saturation measurements of fluorophores in solution using pulsed femtosecond excitation and two-dimensional CCD camera detection. Appl. Opt., in press.
7. Gumy, J.-C. & Vauthey, E. (1996) Picosecond polarization grating study of the effect of excess excitation energy on the rotational dynamics of rhodamine 6G in different electronic states. J. Phys. Chem. 100, 8628-8632.
8. Lakowicz, J.R. (1980) Fluorescence spectroscopic investigations of the dynamic properties of proteins, membranes and nucleic acids. J. Biochem. Biophys. Methods. 2, 90-119.
9. Lakowicz, J.R. (1983) Principles of Fluorescence Spectroscopy. Plenum Press, New York.
10. Lakowicz, J.R. & Berndt, K.W. (1991) Lifetime-selective fluorescence imaging using an rf phase-sensitive camera. Rev. Sci. Instrum. 62 (7), 1727-1734.
11. + and glucose. Sens. Act. B. 11, 133-143.
12. Lippitsch, M.E. & Draxler, S. & Leiner, M.J.P. (1992) Time-domain fluorescence methods as applied to pH sensing. Proc. SPIE, 1204, 798-807.
13. Loudon, R. (1973) The Quantum Theory of Light. 2nd edn. Clarendon Press, Oxford.
14. Müller, M., Ghauharali, R., Visscher, K. & Brakenhoff, G.J. (1995) Double-pulse fluorescence lifetime imaging in confocal microscopy. J. Microsc. 177, 171-179.
15. Sandison, D.R., Williams, R.M., Wells, K.S., Strickler, J. & Webb, W.W. (1995) Handbook of Biological Confocal Microscopy (ed. by J. B. Pawley). Plenum Press, New York.
16. Sanders, R., Gerritsen, H.C., Draaijer, A., Houpt, P.M. & Levine, Y.K. (1994) Fluorescence lifetime imaging of free calcium in single cells. Bioimaging, 2, 131-138.
17. Schubert, M. & Wilhelmi, B. (1986) Nonlinear Optics and Quantum Electronics. John Wiley & Sons, New York.
18. So, P.T.C., French, T., Yu, W.M., Berland, K.M., Dong, C.Y. & Gratton, E. (1995) Time-resolved fluorescence microscopy using two-photon excitation. Bioimaging, 3, 49-63.
19. Sosnowski, T.S., Stephens, P.B. & Norris, T.B. (1996) Production of 30-fs pulses tunable throughout the visible spectral region by a new technique in optical parametric amplification. Opt. Lett. 21, 140-142.
20. Steiner, R.F. (1991) Topics in Fluorescence Spectroscopy. Vol. 2 (ed. by J. R. Lakowicz). Plenum Press, New York.
21. Straume, M., Frasier-Cadoret, S.G. & Johnson, M.L. (1991) Topics in Fluorescence Spectroscopy, Vol. 2 (ed. by J. R. Lakowicz). Plenum Press, New York.
22. Visscher, K., Brakenhoff, G.J. & Visser, T.D. (1994) Fluorescence saturation in confocal microscopy. J. Microsc. 175, 162-165.